LOCUS       sequence-415447-        5336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SoxS activation domain and gRNA targeting 105 region 
            within the synthetic promoter in bacterial cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Naseri G, Raasch H, Charpentier E, Erhardt M
  TITLE     A versatile regulatory toolkit of arabinose-inducible artificial 
            transcription factors for Enterobacteriaceae.
  JOURNAL   Commun Biol. 2023 Oct 3;6(1):1005. doi: 10.1038/s42003-023-05363-3.
  PUBMED    37789111
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-10-3"; volume: "6(1):1005. doi"; pages: " 
            10.1038/s42003-023-05363-3"
COMMENT     Sequence Label: pSJ105
FEATURES             Location/Qualifiers
     source          1..5336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        363..397
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        481..499
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             522..551
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             567..584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(640..657)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      810..856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(909..926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1074..1168)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1304..1322)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1509..1536)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1552..1623)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1656..2333)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             2340..2351
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(2361..2395)
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     promoter        2608..2712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2713..2781,2782..3573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2931..2950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     RBS             4614..4625
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(4655..4939)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(4713..4732)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     rep_origin      join(4965..5336,1..217)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tctcagataa aatatttcta gatttcagtg
      301 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgttacta
      361 gattgacagc tagctcagtc ctaggtataa tactagtcgg ttaccaaagg cgtccttgtt
      421 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
      481 acatgaggat cacccatgtg ctttttttga agcttgggcc cgaacaaaaa ctcatctcag
      541 aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt taaacggtct
      601 ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
      661 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
      721 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
      781 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaactggat ccttactcga gtctagactg
      901 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aactagtgct tggattctca
     1081 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1141 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     1201 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1261 acaaacggca tgatgaacct gaatcgccag ccgagacgaa agggcctcgt gatacgccta
     1321 tttttatagg ttaatgtcat gataataatg gtttcttacc tcccgtgggg aaaaatcatg
     1381 gcaattctgg aagaaatagc gctttcagcc ggcaaaccgg ctgaagccgg atctgcgatt
     1441 ctgataacaa actagcaaca ccagaacagc ccgtttgcgg gcagcaaaac ccgtacccta
     1501 ggtataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
     1561 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     1621 ttgatgcctg gagatcctta ctcgagtttg gatccttaag caccggtgga gtgacgacct
     1681 tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt tgtgggaggt gatgtccagt
     1741 ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt ttttagccat gtaggtggtt
     1801 ttaacttcag cgtcgtagtg accaccgtct ttcagtttca gacgcatttt gatttcacct
     1861 ttcagagcac cgtcttccgg gtacatacgt tcggtggaag cttcccaacc catggttttt
     1921 ttctgcataa ccggaccgtc ggacgggaag ttggtaccac gcagtttaac tttgtagatg
     1981 aactcaccgt cttgcaggga ggagtcctgg gtaacggtaa caacaccacc gtcttcgaag
     2041 ttcataacac gttcccattt gaaaccttcc gggaaggaca gtttcaggta gtccgggatg
     2101 tcagccgggt gtttaacgta agctttggaa ccgtactgga actgcgggga caggatgtcc
     2161 caagcgaacg gcagcggacc acctttggta actttcagtt tagcggtctg ggtaccttcg
     2221 tacggacgac cttcaccttc accttcgatt tcgaactcgt gaccgttaac ggaaccttcc
     2281 atacgaactt tgaaacgcat gaactctttg ataacgtctt cgctactcgc catggtacct
     2341 ttctcctctt taatgaattc gctagcacaa tccctaggac tgagctagct gtcaagtccg
     2401 gaggtatccg gtggaaccca aaggacgcct ttggtaaccg caggacaccg caggatacct
     2461 gaggtcgccc gtggtggccc atggtcacca taggtcaccc ttggcaacca aaggtgtcct
     2521 atggcggccg gaggctgcca taggtctccg gtggataccg taggcgacgt cggaattgcc
     2581 acaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2941 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3421 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3601 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3661 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3721 gtagaaaaga tcaaaggatc ttcaagcagc ataacctttt tccgtgatgg taacttcacg
     3781 gtaaccaaga tgtcgagtta accacccttt agattcataa agcgaaaata atgcggctcc
     3841 aacgtaccca cctaaatgga aacggcgttc actccaagcg aaaaaacccc gccgaagcgg
     3901 ggttttttgc gttaaagccg gtgacggtag tcgctgggag tccggtcaaa ttgacgagcg
     3961 aagacgcgag agaaggtttg ctgcgacaca tagcctaagt ccatggcaat atcgaaaatc
     4021 ggacgttctg ttgtccgcaa ttcaacggca gccaacaaca aacggcgttg acggatatag
     4081 tctcctaatg tttgatgtgt gacggtacga aacatgcgct gtaaatacca cttcgagtaa
     4141 ccacttttct tcgctacaac atcaatatta agaggctgat caatatgctc atcgatccaa
     4201 gcaataaggt cctggatgat tttttggtgg ctcattgaac cacctccgcc gtaaatacca
     4261 ctgttggccg cgatggcaga tggaatcgga tttccatctt tcagaagacc ctgcatcgct
     4321 ttcacaatca actcacagtc gctattcgta gcaaagatcg gaatagtcag ttccatatta
     4381 aggtacgaac gccatgcacc tttagggact tcaactttga tcgtgtactt acggttttgc
     4441 gcactcgatt gacgtacaga acaagtaact ttgtatgctt ggctacggct gttggaggaa
     4501 atccactcgg caatgccgtt agcgaagttg ctaggggcaa cagttacatc accagtacca
     4561 ccattatcca caagaacgaa ctgcgtaaaa ttgctcgcgg gacccataga tcctttctcc
     4621 tctttagatc ttttgaattc ccaaaaaaac gggtatggag aaacagtaga gagttgcgat
     4681 aaaaagcgtc aggtagaatc cgctaatctt atggataaaa atgctatggc atagcaaagt
     4741 gtgacgccgt gcaaataatc aatgtggact tttctgccgt gattatagac acttttgtta
     4801 cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa tgcttttaat aagcggggtt
     4861 accggtttgg ttagcgagaa gagccagtaa aagacgcagt gacggcaatg tctgatgcaa
     4921 tatggacaat tggtttcttc tctgaatggc gggagtatga aaagttgaga tccttttttt
     4981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5101 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaact
//
