LOCUS       sequence-415462-       12939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal mEos 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12939)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 12939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos Cav2.2 pMT2
FEATURES             Location/Qualifiers
     source          1..12939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             190..753
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    940..1074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(977..996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1031..1050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1747..1764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1918..2506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1998..2017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2677..3468,3469..3537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3300..3319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3538..3642)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3710..3728
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3766..3788)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3888..3907
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4101..4123
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4115..4132
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4116..4132
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4142..4471)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4156..4291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4456..4476
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          4940..5169
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5152..5171
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5216..5893
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
       61 gggggggggg gggggggggg gggggggggg gggggggggg gggggggaca gctcagggct
      121 gcgatttcgc gccaaacttg acggcaatcc tagcgtgaag gctggtagga ttttatcccc
      181 gctgccatca tggttcgacc attgaactgc atcgtcgccg tgtcccaaaa tatggggatt
      241 ggcaagaacg gagacctacc ctggcctccg ctcaggaacg agttcaagta cttccaaaga
      301 atgaccacaa cctcttcagt ggaaggtaaa cagaatctgg tgattatggg taggaaaacc
      361 tggttctcca ttcctgagaa gaatcgacct ttaaaggaca gaattaatat agttctcagt
      421 agagaactca aagaaccacc acgaggagct cattttcttg ccaaaagttt ggatgatgcc
      481 ttaagactta ttgaacaacc ggaattggca agtaaagtag acatggtttg gatagtcgga
      541 ggcagttctg tttaccagga agccatgaat caaccaggcc acctcagact ctttgtgaca
      601 aggatcatgc aggaatttga aagtgacacg tttttcccag aaattgattt ggggaaatat
      661 aaacttctcc cagaataccc aggcgtcctc tctgaggtcc aggaggaaaa aggcatcaag
      721 tataagtttg aagtctacga gaagaaagac taacaggaag atgctttcaa gttctctgct
      781 cccctcctaa agctatgcat ttttataaga ccatgggact tttgctggct ttagatcata
      841 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      901 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      961 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1021 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccggc
     1081 caacggtctg gtgacccggc tgcgagagct cggtgtacct gagacgcgag taagcccttg
     1141 agtcaaagac gtagtcgttg caagtccgca ccaggtactg atatcccacc aaaaagtgcg
     1201 gcggcggctg gcggtagagg ggccagcgta gggtggccgg ggctccgggg gcgaggtctt
     1261 ccaacataag gcgatgatat ccgtagatgt acctggacat ccaggtgatg ccggcggcgg
     1321 tggtggaggc gcgcggaaag tcgcggacgc ggttccagat gttgcgcagc ggcaaaaagt
     1381 gctccatggt cgggacgctc tggccggtga ggcgtgcgca gtcgttgacg ctctagaccg
     1441 tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc tggtggataa attcgcaagg
     1501 gtatcatggc ggacgaccgg ggttcgaacc ccggatccgg ccgtccgccg tgatccatgc
     1561 ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag acaacggggg agcgctcctt
     1621 ttggcttcct tccaggcgcg gcggctgctg cgctagcttt tttggcgagc tcgaattaat
     1681 tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1741 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1801 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1861 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1921 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1981 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2041 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2101 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2161 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2221 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2281 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2341 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2401 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2461 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2521 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2581 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2641 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2701 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2761 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2821 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2881 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2941 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3001 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3061 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3121 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3181 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3241 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3301 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3361 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3421 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3481 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3541 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3601 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3661 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3721 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     3781 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     3841 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     3901 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3961 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4021 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     4081 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     4141 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     4201 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     4261 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     4321 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     4381 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     4441 ctggggactt tccacaccct aactgacaca cattccacag gatccggtcg cgcgaatttc
     4501 gagcggtgtt ccgcggtcct cctcgtatag aaactcggac cactctgaga cgaaggctcg
     4561 cgtccaggcc agcacgaagg aggctaagtg ggaggggtag cggtcgttgt ccactagggg
     4621 gtccactcgc tccagggtgt gaagacacat gtcgccctct tcggcatcaa ggaaggtgat
     4681 tggtttatag gtgtaggcca cgtgaccggg tgttcctgaa ggggggctat aaaagggggt
     4741 gggggcgcgt tcgtcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc
     4801 tcgcggttga ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg
     4861 gcctccgaac ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga
     4921 aaacctctcg actgttgggg tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta
     4981 agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc ggtgatgcct
     5041 ttgagggtgg ccgcgtccat ctggtcagaa aagacaatct ttttgttgtc aagcttgagg
     5101 tgtggcaggc ttgagatctg gccatacact tgagtgacaa tgacatccac tttgcctttc
     5161 tctccacagg tgtccactcc caggtccaac tgcaggcgag cctgaattcg gatccatgag
     5221 tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa acgggcacca
     5281 ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct
     5341 tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca
     5401 ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt ttaagcagtc
     5461 gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcat
     5521 tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc gattttatgg
     5581 taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc
     5641 cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattcata tggctttgtt
     5701 gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa
     5761 gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt taagccatga
     5821 caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga
     5881 caatgccaga cgagtccgct tcggggacga gctgggcggc cgctatgggg gcgcgggcgg
     5941 cgcggagcgg gcccggggcg gcggggccgg cggggctggc ggcccgggcc ccggggggct
     6001 gccgccgggc cagcgggtcc tctacaaaca gtcgatcgcg cagcgcgcgc ggaccatggc
     6061 gctgtacaac cccatcccgg tcaagcagaa ctgcttcacc gtcaaccgct cgctcttcgt
     6121 cttcagcgag gacaacgtcg tccgcaaata cgccaagcgc atcaccgagt ggcctccgtt
     6181 cgagtacatg atcctggcca ccatcatcgc caactgcatc gtgctggccc tggagcagca
     6241 cctccctgac ggggacaaga cgcctatgtc cgagcgcctg gatgacaccg agccctactt
     6301 cattgggatc ttctgcttcg aggcggggat caagatcctg gccctgggct ttgttctgca
     6361 caagggctcc tatctgcgga acggctggaa cgtcatggac ttcgtggtag tcctcacggg
     6421 gattcttgcc acagccggaa ccgacttcga cctgcgaacg ctgagggctg tgcgtgtgct
     6481 aaggcccctg aagctggtgt ctgggattcc gagcttgcag gtggtgctca agtccatcat
     6541 gaaggccatg gtcccgctgc tgcagatcgg gctgctgctc ttcttcgcca tcctcatgtt
     6601 tgccatcatc ggcctcgagt tctacatggg caagttccac aaggcctgct tccccaacag
     6661 cacagaccca gatcccgtgg gtgacttccc ctgtggcaag gaggccccgg cccggctctg
     6721 tgagggagac acggagtgcc gggagtactg ggccggaccc aactttggta tcaccaactt
     6781 tgacaacatc ctgtttgcca tcttgaccgt gttccagtgc atcaccatgg agggctggac
     6841 tgacatcctc tacaatacaa acgatgcagc cggcaacacg tggaactggc tctacttcat
     6901 ccctctcatc atcatcggct ccttcttcat gctcaacctg gtgctgggtg tgctctcagg
     6961 agagtttgct aaggagcgcg agagagtgga gaaccgccgc gccttcctga agctccggcg
     7021 gcagcagcag attgagcggg agctcaacgg gtacctggag tggatcttca aggctgagga
     7081 agtcatgctg gctgaggagg acaggaacgc agaggagaag tcccctctgg atgcagtgtt
     7141 gaagagagca gctgccaaga agagcagaag cgacctgatc caggccgagg agggggaggg
     7201 ccggctcaca ggcctctgtg ctcccggttc cccgttcgcc cgtgccagcc tgaaaagcgg
     7261 gaagacggag agctcgtcgt acttccggag gaaggagaag atgttccgct tcttcatccg
     7321 gcgcatggtg aaggcacaga gcttctactg gacggtgctg tgtgtggtgg cactgaacac
     7381 actgtgcgtg gccatggtgc actacaacca gccgcagcgg ctcaccacgg cattgtactt
     7441 tgcagagttt gttttcctgg gtctcttcct cacagagatg tccctgaaga tgtatggcct
     7501 ggggcccagg agctacttcc ggtcctcctt caactgtttt gactttgggg tcatcgtggg
     7561 cagtatcttt gaagtggtct gggctgctgt caagccaggg acctccttcg ggatcagtgt
     7621 tctgcgggct ctgcgcttac tgagaatctt caaggtcacc aaatactgga actccctacg
     7681 gaacctggtg gtgtctctgc tcaactccat gaagtccatc atcagcctcc tcttcctgct
     7741 cttcctgttc attgttgtct ttgctctgct ggggatgcag ctgtttggag gccagttcaa
     7801 ctttaaagac gagaccccaa cgaccaactt tgacaccttc cctgctgcta tcctcaccgt
     7861 cttccagatc ctgaccggag aggactggaa tgcggtcatg taccacggga tcgagtcgca
     7921 aggtggcgtc agcagaggaa tgttctcctc cttctacttc attgtcctca ctctgtttgg
     7981 gaactacacc ctgctgaatg tcttcctggc cattgctgtg gacaaccttg ccaatgctca
     8041 ggagctgacc aaggatgaag aggagatgga agaagcagcc aatcagaaac tcgccttgca
     8101 gaaggccaag gaagtggctg aagtcagccc catgtctgct gcgaacatct ccattgccgc
     8161 caggcagcag aactcggcca aagcgcgctc ggtgtgggag cagcgagcca gccagctgcg
     8221 gctgcagaac ctgagggcca gctgcgaggc gctgtacagc gagatggacc ccgaggagag
     8281 gctgcgctac gccaccgcgc gccacctgcg gcccgacgtg aagacgcacc tggaccggcc
     8341 gctggtggtt gagcccggcc gcgacgctcc gcggggcccc ccggggggga agtcccggcc
     8401 ggatggctcg gaggcccccg agggtgcgga tcccccgcgc aggcatcacc ggcaccgaga
     8461 caaggacaag gctcccgcca cggtcccctc ggcgggggaa caggacagag ccgaagctct
     8521 gagggcagag ggcggggagc tggggccccg cgaggagcgg gggcggccac gccgcagccg
     8581 cagcaaggag gccccgggcg ccccggaggt gcgcagcgat cgcggccgtg gcccgtgtcc
     8641 tgaaggtggt cggcgccacc accggcgcgg gtcaccggag gaggcagctg agagggagcc
     8701 ccgccgccac cgcgcgcacc ggcacgggcc cgacccgggc aaggagggcc cagcgtccgg
     8761 gaccaggggc gagcggcgcg cacgacaccg cacgggcccc cgggcgtgcc cccgggaggc
     8821 tgagagcagc gaggagcctg cgcggcggca ccgggctcgg cacaaggcgc cgcccaccca
     8881 ggagaccgcg gagaaggaca aggaggctgc cgagaagggg ggcgaggcca cggaggccga
     8941 gaaggacaag gaggctcgga accaccagcc caaggagctg ccctgtgacc tggaggccat
     9001 cggcatgctg ggagtgggcg ctgtgcatac gctgcccagc acctgtctgc agaaggtgga
     9061 ggagcagcca gaggatgcgg acaaccagcg gaacgtcacc cgcatgggta gtcagccccc
     9121 ggacaccagc accactgtgc acattcctgt gacgctgacc ggcccccccg gggagaccac
     9181 ggtagtgccc agtggaaacg tggacctgga aagtcaagca gaggggaaga aggaggtgga
     9241 aaccagcgat gtgatgagga gcggccctcg gcccattgtc ccgtacagtt ccatgttctg
     9301 tttaagccca accaacctgc tgcgccgctg ctgccactac attgtgacca tgcgatactt
     9361 cgagatggtc atcctcgtgg tcatcgccct gagcagcatc gccctggctg ccgaggaccc
     9421 cgtgagaaca gactctccca ggaataatgc tctgaagtac atggactaca tcttcacagg
     9481 cgtcttcacc ttcgagatgg tgataaagat gatcgatttg ggactgctgc tacaccctgg
     9541 agcctatttc cgggatctgt ggaacattct ggacttcatt gtggtcagtg gggccctggt
     9601 ggcatttgct ttctcaggat ccaaagggaa agacatcagc accatcaaat ccctgagagt
     9661 cctgcgtgtg ctgcggccac tcaagaccat caagcggctg cccaagctca aggccgtgtt
     9721 tgactgtgtg gtgaactcac tgaaaaacgt cctcaacatc ctgatcgtct acatgctctt
     9781 catgttcatc tttgccgtca ttgccgtgca gctcttcaaa gggaagttct tctactgcac
     9841 ggacgaatcc aaggagctgg agcgtgactg caggggtcag tatttggatt atgagaagga
     9901 ggaagtggaa gctcaaccga ggcagtggaa gaaatatgac ttccactatg acaacgtgct
     9961 ctgggctctg ctaacgctct tcacggtgtc cacaggggaa gggtggccca tggtgctgaa
    10021 acactctgtg gatgccacct acgaggagca gggcccgagc cccgggtacc gcatggagct
    10081 ctccatcttc tacgtggtct actttgtggt ctttcccttc ttctttgtca acatcttcgt
    10141 ggccttgatc atcatcacct tccaggagca gggggacaag gtgatgtccg agtgcagcct
    10201 ggagaagaac gagagggctt gcatcgactt tgccatcagc gccagacccc tgacgcggta
    10261 catgccccag aacaagcagt cgttccagta taagacgtgg acgttcgtgg tgtccccacc
    10321 ttttgagtac ttcatcatgg ctatgatcgc cctcaacact gtggtgctga tgatgaagtt
    10381 ctacgatgcg ccgtacgagt atgagctaat gctgaaatgc ctgaacatcg tgttcacgtc
    10441 catgttctcc atggagtgtg tgctgaagat catcgccttc ggggtgctga actatttcag
    10501 agatgcctgg aatgtctttg actttgtcac ggtgttggga agtattactg atattttggt
    10561 aacagagatt gcgaacaact tcatcaacct cagcttcctg cgcctgttcc gggccgcgcg
    10621 gctcatcaag ctgctgcgcc agggctacac catccgcatc ctgctttgga ccttcgtgca
    10681 gtccttcaag gcgctgccct acgtgtgtct cctcatcgcc atgctcttct tcatctacgc
    10741 catcattggc atgcaggttt ttgggaacat cgccctggac gatgacacca gcatcaacag
    10801 gcacaataac ttccggacgt ttctgcaggc cttgatgttg ctgttcagga gcgccacggg
    10861 ggaggcctgg cacgagataa tgctgtcgtg cctcagcagc cgggcctgcg acgagcactc
    10921 caatgccagc gagtgcggca gcgactttgc ctacttttac ttcgtctcct tcatcttcct
    10981 ctgctccttt ctgatgttga acctctttgt ggctgtgatc atggacaatt ttgagtacct
    11041 tacacgggac tcttccatct tagggcctca ccacctggat gagttcatcc gggtctgggc
    11101 tgaatacgac ccggccgcgt gtgggcgcat cagttacagt gacatgtttg agatgctgaa
    11161 gcacatgtcc ccacccctgg ggctggggaa gaagtgccct gctcgagtcg cgtacaagcg
    11221 cctggtccgc atgaacatgc ccatctccag tgaggacatg acggtgcact tcacatccac
    11281 gctgatggct ctcattcgga cagcgctgga catcaagctg gcaccagctg ggaccaagca
    11341 gcaccagtgc gacgcagagc tgaggaagga gatttcctgt gtgtgggcca atctgcccca
    11401 gaagaccctg gacttactgg taccacccca taagcctgac gagatgacgg tgggcaaagt
    11461 ctatgcagct ctgatgatct tcgacttcta caaacagaac aaaaccagca gagaccagac
    11521 tcagcaagcc cccggaggcc tgtcccagct gggccccgtg tccctgttcc acccgctgaa
    11581 ggccacgctg gagcagactc agccggcgct gcgtggagcc cgggctttcc tgcggcagaa
    11641 gagttctgcc tccctgagca acggcggggc cgtacaaacc caggagagcg gcatcaagga
    11701 atcggtttct tggggcactc agaggaccca ggatgtgctt tgcgaggcca gggcacccct
    11761 ggagcggggc cactcagctg agatcccagt cgggcagcct gggacactgg ctgtagatgt
    11821 ccagatgcag aacatgactc tgagtggccc tgacgccgag ccgcagccag ggctggagag
    11881 ccagggccga gccgcctcca tgccccgcct ggccgcagaa acacagcccg cgcctgacgc
    11941 cagccccatg aagcgctcca tctccacgct ggccccgcgc ccccacacgg cccggctcgg
    12001 cagcaccgcg ctggaccgcc cagcccccag ccaggccccg caccatcacc accaccgctg
    12061 ccaccgccgc agggacagga agcagcggtc cctggagaag ggtcccagcc tgtctgctga
    12121 cacagatggg gcccccgaca gcacggtggg gccaggactg cccacggggg agggcccccc
    12181 cggctgcagg cgggagcggg agcgcaggca ggagcggggc cggtcccagg agcggaggca
    12241 gccctcgtcc tcctcctcgg agaagcatcg cttctactcc tgtgaccgct tcgggggccg
    12301 cgaaccccca cagcccaagc cctccctcag tagtcacccc acgtcgccga cggccgggca
    12361 ggagccggga ccccacccgc aggggagtgg ttcggttcac gggagccccc tgctgtccac
    12421 atctggtgct agcaccccgg gccgggggcg gaggcagctc ccccagaccc ccttgacccc
    12481 ccgccccagc gtcacctaca agacggccaa ctcctcgcct gtccacttcg ccggggcacc
    12541 gagcggcctc cccgccttct cccccggccg gctcagccgg ggcctctcag aacacaacgc
    12601 cctgctgcag agggacccgc tcagccggcc cctggcgccc ggctcgcgca tcggctccga
    12661 cccctacctg gggcagcggc tggacagcga ggcgcccgcc cgtgccctgc ccgaggacgc
    12721 gcccgccttt gaggagaccg cggccagcaa ctcgggccgc tcctcccgga cctcgtacgt
    12781 gtcctcactg acctcgcagc ccccgccgct gcgccgcgtg cccaacggct accactgcac
    12841 tctggggctg ggcgggggcg gccgggcgcg gcgcggctgc caccaccccg accgcgaccg
    12901 ccggtgctag actagttcta gcaagcttcg acgaattnc
//
