LOCUS       sequence-415466-        4751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dimerization dependent fluorescent protein B anchored to cytosolic 
            face of the plasma membrane with the N-terminal targeting sequence 
            of human GAP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Miner GE, Smith SY, Showalter WK, So CM, Ragusa JV, Powers AE, 
            Zanellati MC, Hsu CH, Marchan MF, Cohen S
  TITLE     Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for
            Visualizing Membrane Contact Site Dynamics.
  JOURNAL   Contact (Thousand Oaks). 2024 Feb 4;7:25152564241228911. doi: 
            10.1177/25152564241228911. eCollection 2024 Jan-Dec.
  PUBMED    38327561
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Contact 
            (Thousand Oaks)."; date: "2024-02-4"; volume: "7:25152564241228911. 
            doi"; pages: " 10.1177/25152564241228911. eCollection 2024 Jan-Dec"
COMMENT     Sequence Label: PM-B
FEATURES             Location/Qualifiers
     source          1..4751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     474..494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(776..794)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1044..1063
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1238..1261
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1494..1615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1531..1550)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1585..1604
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1622..2077)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1759..1780)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1971..1990
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2104..2208
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2210..2567
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2210..2401
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2233..2253)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2418..2553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2602..3396
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2602..3396
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2656..2675)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3266..3285
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3584..3603)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3628..3675
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4004..4592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4493..4512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatccgctag cgctaccggt cgccaccatg ctgtgctgta tgagaagaac caaacaggtt
      601 gaaaaaaatg atgacgacca aaagattggt ggtggtggta gtggtggtgg tggtagtgct
      661 gccaccatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
      721 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      781 ctgaaggtga ccaagggcgg ccccctgccc tttgcctggg acatcctgtc cccccagatc
      841 atgtacggct ccaaagcgta cgtgaggcac cccgccgaca tccccgatta caagaagctg
      901 cccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaagacgg cggtctggtg
      961 accgttaccc aggactcctc cctgcaggac ggcacgctga tctgcaaggt gaagattcgc
     1021 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     1081 tccaccgaga tgctgtaccc caaggacggc gtgctgaagg gccatagcta tcaggccctg
     1141 aagctgaagg acggcggcca ctacctggtg gagttcgaga ccatctacat ggccaagaag
     1201 cccgtgcaac tgcccggcga ttactgtgtg gacaccaagc tggacatcac ctcccacaac
     1261 gaggactaca ccatcgtgga acagtacggg cgctccgagg gccgccaccg tctgggcatg
     1321 gacgagcggt acaagtgact gcagtcgacg gtaccgcggg cccgggatcc accggatcta
     1381 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     1441 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1501 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1561 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     1621 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     1681 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     1741 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     1801 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     1861 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     1921 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     1981 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2041 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     2101 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2161 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     2221 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2281 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     2341 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     2401 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     2461 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     2521 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     2581 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     2641 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2701 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     2761 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     2821 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2881 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     2941 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3001 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3061 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     3121 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     3181 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3241 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3301 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3361 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     3421 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     3481 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     3541 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     3601 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     3661 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     3721 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     3781 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     3841 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4141 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4381 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4681 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     4741 gttcatagcc c
//
