LOCUS       sequence-415477-        8354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Zeng H, Yuan Q, Peng F, Ma D, Lingineni A, Chee K, Gilberd P, Osikpa
            EC, Sun Z, Gao X
  TITLE     A split and inducible adenine base editor for precise in vivo base 
            editing.
  JOURNAL   Nat Commun. 2023 Sep 11;14(1):5573. doi: 10.1038/s41467-023-41331-5.
  PUBMED    37696818
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-11"; volume: "14(1):5573. doi"; pages: " 
            10.1038/s41467-023-41331-5"
COMMENT     Sequence Label: sABE v3.22_C
FEATURES             Location/Qualifiers
     source          1..8354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      872..1460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1361..1380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1614..1631
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1790..1817)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     enhancer        1922..2301
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2302..2505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2456..2476
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2536..2556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3703..3726,3727..3729,3730..7803)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3703..7803
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7810..7830
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7881..7899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7882..7899)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7919..7936)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7925..8149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8259..8278)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(8270..8354,1..725)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(join(8337..8354,1..2))
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
       61 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
      121 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
      181 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
      241 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
      301 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtcttgctc
      361 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
      421 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
      481 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
      541 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
      601 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
      661 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
      721 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
      781 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
      841 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      901 gctgcttgcg aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      961 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1021 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1081 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1141 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1201 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1261 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1321 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1381 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1441 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1501 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1561 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1621 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1681 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     1741 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     1801 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     1861 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcccat ctcaacgcgt
     1921 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     1981 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2041 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2101 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2161 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2221 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2281 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2341 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2401 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2461 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     2521 gaacccactg ctatgccaaa gaaaaagagg aaggtgagcc ccaagtacac caagagcgtg
     2581 ctgaagaagg gtgacaagac caacttcccc aaaaagggag atgtggtgca ctgctggtac
     2641 accggcacgc tgcaagacgg caccgtgttc gacaccaaca tccagaccag cgctaaaaag
     2701 aagaaaaacg ccaaacccct gtctttcaaa gtgggcgtgg gcaaggtgat caggggctgg
     2761 gacgaagctc tgctgaccat gagcaagggc gagaaggcca ggctggagat cgagcccgag
     2821 tgggcctatg gcaagaaagg acagccagac gccaagatcc cgcccaacgc aaaactgacc
     2881 tttgaggtgg aactggttga tatcgatggt ggcggctcag gtggcgggag cggaggcggg
     2941 agctctccca agtacaccaa gagtgttctg aagaaaggcg acaagaccaa cttccccaaa
     3001 aagggagatg tggtgcactg ctggtacacc ggcacgctgc aagacggcac cgtgttcgac
     3061 accaacatcc agaccagcgc taaaaagaag aaaaacgcca aacccctgtc tttcaaagtg
     3121 ggcgtgggca aggtgatcag gggctgggac gaagctctgc tgaccatgag caagggcgag
     3181 aaggccaggc tggagatcga gcccgagtgg gcctatggca agaaaggaca gccagacgcc
     3241 aagatcccgc ccaacgcaaa actgaccttt gaggtggaac tggttgatat cgatggtgga
     3301 ggctctggag gaagcagcgg cggcagctcg ggcgacgcca ccctgtacgt gacattcgag
     3361 ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc
     3421 gtgaggaact caaaaagagg cgccgcaggc tccctgatga acgtgctgaa ctaccccggc
     3481 atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg
     3541 tgcgatttct atcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc
     3601 atcaactccg gaggatctag cggaggatcc tctggcagcg agacaccagg aacaagcgag
     3661 tcagcaacac cagagagcag tggcggcagc agcggcggca gcgacaagaa gtacagcatc
     3721 ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
     3781 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
     3841 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
     3901 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
     3961 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
     4021 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
     4081 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
     4141 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
     4201 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     4261 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
     4321 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
     4381 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
     4441 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     4501 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     4561 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     4621 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     4681 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     4741 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     4801 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     4861 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
     4921 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     4981 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     5041 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     5101 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     5161 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     5221 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     5281 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     5341 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     5401 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     5461 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     5521 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     5581 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     5641 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     5701 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     5761 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     5821 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     5881 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     5941 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     6001 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     6061 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
     6121 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     6181 ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg
     6241 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
     6301 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     6361 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     6421 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     6481 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     6541 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     6601 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     6661 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     6721 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     6781 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     6841 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     6901 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     6961 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     7021 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     7081 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
     7141 aaagtggaaa agggcaagtc caaaaaactg aagagtgtga aagagctgct ggggatcacc
     7201 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     7261 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     7321 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
     7381 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     7441 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     7501 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     7561 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     7621 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     7681 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
     7741 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt
     7801 gacagcggcc ccaagaagaa gaggaaagtc taaactacga accccgggta aatgcatgaa
     7861 ttcgatctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     7921 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7981 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     8041 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     8101 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     8161 gaaagggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     8221 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     8281 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     8341 taaatgggct cgcg
//
