LOCUS       sequence-41548-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Ren XD, Kiosses WB, Schwartz MA
  TITLE     Regulation of the small GTP-binding protein Rho by cell adhesion and
            the cytoskeleton.
  JOURNAL   EMBO J. 1999 Feb 1. 18(3):578-85.
  PUBMED    9927417
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            1999 Feb 1. 18(3):578-85."
COMMENT     Sequence Label: GST-RBD
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(334..356)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(501..519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        587..691
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcggatctgg ttccgcgtgg atccatcctg gaggacctca atatgctcta catccggcag
       61 atggcactca gcctggagga cacagagctg cagaggaaac tagatcatga gatccggatg
      121 agggatgggg cctgcaagct gctggcagcc tgctcccagc gagagcaggc tctggaagcc
      181 accaagagcc tgctggtgtg caacagccgt attctcagct acatgggtga gctgcagcgg
      241 cgaaaggagg cccaggtgct ggagaagaca ggctagaatt catcgtgact gactgacgat
      301 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      361 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      421 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
      481 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      541 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      841 cagcggta
//
