LOCUS       sequence-415522-        4008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Zeng H, Yuan Q, Peng F, Ma D, Lingineni A, Chee K, Gilberd P, Osikpa
            EC, Sun Z, Gao X
  TITLE     A split and inducible adenine base editor for precise in vivo base 
            editing.
  JOURNAL   Nat Commun. 2023 Sep 11;14(1):5573. doi: 10.1038/s41467-023-41331-5.
  PUBMED    37696818
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-11"; volume: "14(1):5573. doi"; pages: " 
            10.1038/s41467-023-41331-5"
COMMENT     Sequence Label: sABE v3.22_N
FEATURES             Location/Qualifiers
     source          1..4008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      639..1227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1128..1147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1381..1398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1557..1584)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     enhancer        1689..2068
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2069..2272
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2223..2243
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2314..2333
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2314..2332
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2382..2402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(3302..3320)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3303..3320)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3340..3357)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3346..3570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3680..3699)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(3691..4008,1..492)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3758..3777)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
       61 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
      121 cttgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
      181 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
      241 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
      301 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
      361 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
      421 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
      481 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
      541 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
      601 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      661 gtaatctgct gcttgcgaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      721 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      781 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      841 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      901 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      961 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1021 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1081 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1141 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1201 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1261 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1321 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1381 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     1441 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     1501 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     1561 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     1621 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcccatctc
     1681 aacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     1741 tcatagccca tatattgagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1801 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1861 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1921 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1981 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2041 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     2101 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     2161 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     2221 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     2281 gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg
     2341 ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag
     2401 tctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga
     2461 gggcacggga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga
     2521 tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta
     2581 tggccctgag acagggcggc ctggtcatgc agaactacag actgattgga ggctctggag
     2641 gaagcagcgg cggcagctcg ggcggtggcg gctcgagcat cctgtggcat gagatgtggc
     2701 acgagggcct ggaggaggcc agcaggctgt atttcggcga gaggaatgtg aagggcatgt
     2761 tcgaggtgct ggagcccctg cacgccatga tggaaagggg cccacagacc ctgaaggaaa
     2821 ctagcttcaa ccaggcctac ggcagggacc tgatggaggc ccaggagtgg tgcaggaagt
     2881 acatgaagag cggcaacgtg aaggatctga cccaggcttg ggacttgtac taccacgtgt
     2941 tcaggaggat cagctcgggc ggtggcggct cgagcatcct gtggcatgag atgtggcacg
     3001 agggcctgga ggaggccagc aggctgtatt tcggcgagag gaatgtgaag ggcatgttcg
     3061 aggtgctgga gcccctgcac gccatgatgg aaaggggccc acagaccctg aaggaaacta
     3121 gcttcaacca ggcctacggc agggacctga tggaggccca ggagtggtgc aggaagtaca
     3181 tgaagagcgg caacgtgaag gatctgaccc aggcttggga cttgtactac cacgtgttca
     3241 ggaggatcag ctaaactacg aaccccgggt aaatgcatga attcgatcta gagggcccta
     3301 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
     3361 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3421 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3481 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3541 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagggcc cgtgtctcaa
     3601 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     3661 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     3721 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     3781 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     3841 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3901 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3961 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccag
//
