LOCUS       sequence-41554-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Soriano P.
  TITLE     Generalized lacZ expression with the ROSA26 Cre reporter strain.
  JOURNAL   Nat Genet. 1999 Jan;21(1):70-1.
  PUBMED    9916792
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "1999-01"; volume: "21(1)"; pages: "70-1"
COMMENT     Sequence Label: pROSA26-5'
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     48..64
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(258..274)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(259..275)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(304..324)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(304..322)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(343..359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(343..359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(356..378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    367..383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(391..397,398..415,416..421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    436..457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(574..591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgattct agaactagtg
       61 gatcccccgg gctgcaggaa ttcgattccc tacaacccga attcctacag ctctctggct
      121 cctcagagag cctcggctag gtaggggatc gggactctgg cgggagggcg gcttggtgcg
      181 tttgcgggga tgggcggccg cggcaggccc tccgagcgtg gtggagccgt tctgtgagac
      241 agccggatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccagcttttg
      301 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
      361 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      421 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      481 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      541 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat acc
//
