LOCUS       sequence-415563-        8474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses 
            PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVse
            q-SbMVp-SbMVseq-FRB-VP16 component in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: 
            pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-S
            bMVseq-SbMVp-SbMVseq-FRB-VP16
FEATURES             Location/Qualifiers
     source          1..8474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1014..1032
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1014..1031
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     sig_peptide     1051..1110
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1114..1143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1147..1293
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             2176..2202
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2212..2238
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3013..3033
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3034..3651
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3104..3123)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             3658..3978
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4801..5079
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             5236..5469
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(5481..5500)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5482..5500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5505..5639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5529..5548)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5583..5602
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5752..5772)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5752..5770)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5791..5807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5791..5807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5804..5826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        complement(join(5839..5845,5846..5863,5864..5869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5884..5905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6022..6039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6192..6780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6272..6291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6951..7742,7743..7811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7574..7593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7812..7916)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7942..8397)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     8291..8310
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct ttcatttagg
     1021 tgacactata gaatacaagc tacttgttct atggagacag acacactcct gctatgggta
     1081 ctgctgctct gggttccagg ttccactggt gacgaacaaa aactcatctc agaagaggat
     1141 ctgaatgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt
     1201 aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc
     1261 atcctcatca tgctttggca gaagaagcca cgtggtggct ctggaggact cgagaagtcc
     1321 atgtccagca tggtgagcga tactagctgt accttcccat catctgacgg aatcttctgg
     1381 aagcactgga ttcagactaa ggacggccag tgtggcagcc cactggtgag cacacgagac
     1441 ggattcatcg tggggattca cagcgcctcc aactttacaa acaccaataa ctatttcacc
     1501 tcagtgccaa agaactttat ggagctgctg accaaccagg aggcccagca gtgggtgagc
     1561 gggtggcgcc tgaacgccga ttccgtgctg tggggcgggc acaaggtgtt tatgaccgca
     1621 tgcggtggcg gtggctctgg aggtggtggg tccggaggag gaggtggcgg ttccggcgga
     1681 ggtgggtctg gtggaggtgg atctggaggt ggtggatcag gaggaggagg ttcaggcggc
     1741 cgcgagtttc tggcaaccac actggaacgg atcgagaaaa atttcgtgat tactgatccg
     1801 agactgcctg acaacccaat catttttgcg agcgattcct tcctgcagct gacagaatat
     1861 tctcgggaag agatcctggg gcgcaattgc cgttttctgc agggacccga gacagaccgt
     1921 gccactgttc ggaaaatcag agatgctatt gacaaccaga ctgaagtgac cgttcagctg
     1981 atcaattata ccaagagcgg caagaagttc tggaacgtgt tccacctgca gccgatgcgc
     2041 gattataagg gcgacgtcca gtacttcatt ggcgtgcagc tggatggcac cgaacgtctt
     2101 catggcgccg ctgagcgtga ggcggtctgc ctgatcaaaa agacagcctt tcagattgct
     2161 ggatccatgc ttcaacttcc tcctcttgaa cgtcttactc tcatgttgca gctaccacca
     2221 ttagagagac taacactgat gggcgagagc ctgttcaagg gacctaggga ctacaaccct
     2281 atcagtagca caatttgtca cctgaccaac gagagtgatg gccacacaac aagcctgtac
     2341 ggcatcgggt tcggaccctt tatcatcacc aacaagcacc tgttcaggcg gaataatggc
     2401 actctgctgg tgcagagcct gcacggggtg ttcaaagtga agaacacaac cactctgcag
     2461 cagcacctga tcgatgggcg ggatatgatc atcattagga tgcccaagga cttcccccct
     2521 tttcctcaga aactgaagtt ccgagagccc cagagagagg agagaatctg tctggtgacc
     2581 acaaactttc agaccgagtt tctggcaacc acactggaac ggatcgagaa aaatttcgtg
     2641 attactgatc cgagactgcc tgacaaccca atcatttttg cgagcgattc cttcctgcag
     2701 ctgacagaat attctcggga agagatcctg gggcgcaatt gccgttttct gcagggaccc
     2761 gagacagacc gtgccactgt tcggaaaatc agagatgcta ttgacaacca gactgaagtg
     2821 accgttcagc tgatcaatta taccaagagc ggcaagaagt tctggaacgt gttccacctg
     2881 cagccgatgc gcgattataa gggcgacgtc cagtacttca ttggcgtgca gctggatggc
     2941 accgaacgtc ttcatggcgc cgctgagcgt gaggcggtct gcctgatcaa aaagacagcc
     3001 tttcagattg ctgagaacct gtacttccag ggctctagat tagataaaag taaagtgatt
     3061 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     3121 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     3181 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     3241 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     3301 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     3361 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     3421 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     3481 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     3541 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     3601 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc caccggtgga
     3661 gtgcaggtgg aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc
     3721 tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aatttgattc ctcccgggac
     3781 agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa
     3841 ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc
     3901 tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg
     3961 gagcttctaa aactggaagg aggttctgag tctgtttctc ttcagggagg tggatctatg
     4021 agtaagagcg tgtacaaggg cctgagggac tacagcggca tcagcaccct gatctgccag
     4081 ctgaccaaca gcagcgacgg ccacaaggag accatgttcg gcgtgggcta cggcagcttc
     4141 atcatcacca acggccacct gttcaggagg aacaacggca tgctgaccgt gaagacctgg
     4201 cacggcgagt tcgtgatcca caacaccacc cagctgaaga tccacttcat ccagggcagg
     4261 gacgtgatcc tgatcaggat gcccaaggac ttccccccct tcggcaagag gaacctgttc
     4321 aggcagccca agagggagga gagggtgtgc atggtgggca ccaacttcca ggagaagagc
     4381 ctgagggcca ccgtgagcga gagcagcatg atcctgcccg agggcaaggg cagcttctgg
     4441 atccactgga tcaccaccca ggacggcttc tgcggcctgc ccctggtgag cgtgaacgac
     4501 ggccacatcg tgggcatcca cggcctgacc agcaacgaca gcgagaagaa cttcttcgtg
     4561 cccctgaccg acggcttcga gaaggagtac ctggagaacg ccgacaacct gagctgggac
     4621 aagcactggt tctgggagcc cagcaagatc gcctggggca gcctgaacct ggtggaggag
     4681 cagcccaagg aggagttcaa gatcagcaag ctggtgagcg acctgttcgg caacaccgtg
     4741 accgtgcagg gaggttctga gagcgtgagc ctgcagggcg gtggtagtat ggcttctcga
     4801 atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg
     4861 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg
     4921 ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag
     4981 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cctccaagcc
     5041 tgggacctct attatcatgt gttccgacga atctcaaaga ctagtgcgta cagccgcgcg
     5101 cgtacgaaaa acaattacgg gtctaccatc gagggcctgc tcgatctccc ggacgacgac
     5161 gcccccgaag aggcggggct ggcggctccg cgcctgtcct ttctccccgc gggacacacg
     5221 cgcagactat cgacggcccc cccgaccgat gtcagcctgg gggacgagct ccacttagac
     5281 ggcgaggacg tggcgatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg
     5341 ggggacgggg attccccggg tccgggattt accccccacg actccgcccc ctacggcgct
     5401 ctggatatgg ccgacttcga gtttgagcag atgtttaccg atgcccttgg aattgacgag
     5461 tacggtgggt aggctagcct ccctatagtg agtcgtatta cgtagatcca gacatgataa
     5521 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     5581 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     5641 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     5701 aattcgcgtc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg
     5761 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5821 tccgctcaga attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc
     5881 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5941 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6001 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtca ttcggctgcg
     6061 ggagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7561 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7921 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7981 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     8041 aatcaaaaga atagaccgag atagggttga gtgttgttcc ggtttggaac aagagtccac
     8101 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     8161 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     8221 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     8281 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     8341 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     8401 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8461 tacgccagtc gacc
//
