LOCUS       sequence-415571-        7112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses LAUNCHERsbmv component in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: 
            pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA
FEATURES             Location/Qualifiers
     source          1..7112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1014..1032
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1014..1031
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     sig_peptide     1051..1110
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1114..1143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1147..1293
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             2251..2277
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2287..2313
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(3106..3723,3724..4110)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIE
                     GLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHAD
                     ALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3176..3195)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4119..4138)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4120..4138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4143..4277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4167..4186)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4221..4240
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4390..4410)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4390..4408)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4429..4445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4429..4445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4442..4464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4453..4469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4477..4483,4484..4501,4502..4507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4522..4543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4660..4677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4830..5418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4910..4929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5589..6380,6381..6449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6212..6231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6450..6554)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6580..7035)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6717..6738)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6929..6948
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct ttcatttagg
     1021 tgacactata gaatacaagc tacttgttct atggagacag acacactcct gctatgggta
     1081 ctgctgctct gggttccagg ttccactggt gacgaacaaa aactcatctc agaagaggat
     1141 ctgaatgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt
     1201 aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc
     1261 atcctcatca tgctttggca gaagaagcca cgtggtggct ctggaggact cgagaagagc
     1321 ctgagggcca ccgtgagcga gagcagcatg atcctgcccg agggcaaggg cagcttctgg
     1381 atccactgga tcaccaccca ggacggcttc tgcggcctgc ccctggtgag cgtgaacgac
     1441 ggccacatcg tgggcatcca cggcctgacc agcaacgaca gcgagaagaa cttcttcgtg
     1501 cccctgaccg acggcttcga gaaggagtac ctggagaacg ccgacaacct gagctgggac
     1561 aagcactggt tctgggagcc cagcaagatc gcctggggca gcctgaacct ggtggaggag
     1621 cagcccaagg aggagttcaa gatcagcaag ctggtgagcg acctgttcgg caacaccgtg
     1681 accgtgcaga ccgcatgcgg tggcggtggc tctggaggtg gtgggtccgg aggaggaggt
     1741 ggcggttccg gcggaggtgg gtctggtgga ggtggatctg gaggtggtgg atcaggagga
     1801 ggaggttcag gcggccgcga gtttctggca accacactgg aacggatcga gaaaaatttc
     1861 gtgattactg atccgagact gcctgacaac ccaatcattt ttgcgagcga ttccttcctg
     1921 caactgacag aatattctcg ggaagagatc ctggggcgca attgccgttt tctgcaagga
     1981 cccgagacag accgtgccac tgttcggaaa atcagagatg ctattgacaa ccagactgaa
     2041 gtgaccgttc agctgatcaa ttataccaag agcggcaaga agttctggaa cgtgttccac
     2101 ctgcaaccga tgcgcgatta taagggcgac gtccagtact tcattggcgt gcagctggat
     2161 ggcaccgaac gtcttcatgg cgccgctgag cgtgaggcgg tctgcctgat caaaaagaca
     2221 gcctttcaga ttgctggatc catgcttcaa cttcctcctc ttgaacgtct tactctcatg
     2281 ttgcagctac caccattaga gagactaaca ctgagtaaga gcgtgtacaa gggcctgagg
     2341 gactacagcg gcatcagcac cctgatctgc cagctgacca acagcagcga cggccacaag
     2401 gagaccatgt tcggcgtggg ctacggcagc ttcatcatca ccaacggcca cctgttcagg
     2461 aggaacaacg gcatgctgac cgtgaagacc tggcacggcg agttcgtgat ccacaacacc
     2521 acccagctga agatccactt catccagggc agggacgtga tcctgatcag gatgcccaag
     2581 gacttccccc ccttcggcaa gaggaacctg ttcaggcagc ccaagaggga ggagagggtg
     2641 tgcatggtgg gcaccaactt ccaggaggaa ttcctggcaa ccacactgga acggatcgag
     2701 aaaaatttcg tgattactga tccgagactg cctgacaacc caatcatttt tgcgagcgat
     2761 tccttcctgc agctgacaga atattctcgg gaagagatcc tggggcgcaa ttgccgtttt
     2821 ctgcagggac ccgagacaga ccgtgccact gttcggaaaa tcagagatgc tattgacaac
     2881 cagactgaag tgaccgttca gctgatcaat tataccaaga gcggcaagaa gttctggaac
     2941 gtgttccacc tgcagccgat gcgcgattat aagggcgacg tccagtactt cattggcgtg
     3001 cagctggatg gcaccgaacg tcttcatggc gccgctgagc gtgaggcggt ctgcctgatc
     3061 aaaaagacag cctttcagat tgctgagagc gtgagcctgc aaggttctag attagataaa
     3121 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
     3181 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     3241 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
     3301 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     3361 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     3421 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     3481 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
     3541 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     3601 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     3661 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     3721 tccgcgtaca gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga gggcctgctc
     3781 gatctcccgg acgacgacgc ccccgaagag gcggggctgg cggctccgcg cctgtccttt
     3841 ctccccgcgg gacacacgcg cagactgtcg acggcccccc cgaccgatgt cagcctgggg
     3901 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     3961 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     4021 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     4081 gcccttggaa ttgacgagta cggtgggtag gctagcctcc ctatagtgag tcgtattacg
     4141 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4201 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4261 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4321 aggtgtggga ggttttttaa ttcgcgtccg cggcgccaat gcattgggcc cggtacccag
     4381 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     4441 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa
     4501 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4561 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4621 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4681 ctcggtcgtt cggctgcggg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4741 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4801 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4861 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4921 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4981 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5041 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5101 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5161 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5221 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5281 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5341 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5401 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5461 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5521 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5581 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5641 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5701 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5761 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5821 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5881 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5941 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6001 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6061 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6121 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6181 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6241 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6301 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6361 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6421 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6481 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6541 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     6601 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6661 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6721 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6781 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6841 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6901 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6961 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     7021 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     7081 tgcgggcctc ttcgctatta cgccagtcga cc
//
