LOCUS       sequence-415575-        8175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON 
            and SEAP reporter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: 
            pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-
            SEAP
FEATURES             Location/Qualifiers
     source          1..8175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    122..140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    158..176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    194..212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    230..248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    266..284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    302..320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    338..356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    368..459
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(400..419)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    473..521
                     /note="synthetic polyadenylation signal"
     promoter        549..1533
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1555..1573
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1555..1572
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     sig_peptide     1592..1651
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1655..1684
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1688..1834
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             2717..2743
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2753..2779
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3554..3574
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3575..4318
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="SRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTD
                     SMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    4435..4516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4527..4544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4698..5286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4778..4797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5457..6248,6249..6317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6080..6099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6318..6422)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6455..6536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6656..8158)
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     promoter        complement(join(8172..8175,1..364))
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
ORIGIN
        1 cgagggtagg aagtggtacg gaaagttggt ataagacaaa agtgttgtgg aattgaagtt
       61 tactcaaaaa atcagcactc ttttataggc gccctggttt acataagcaa agcttatacg
      121 ttctctatca ctgataggga gtaaactgga tatacgttct ctatcactga tagggagtaa
      181 actgtagata cgttctctat cactgatagg gagtaaactg gtcatacgtt ctctatcact
      241 gatagggagt aaactcctta tacgttctct atcactgata gggagtaaag tctgcatacg
      301 ttctctatca ctgataggga gtaaactctt catacgttct ctatcactga tagggagtaa
      361 actcgagaga gaaatgttct ggcacctgca cttgcactgg ggacagccta ttttgctagt
      421 ttgttttgtt tcgttttgtt ttgatggaga gcgtatgtta gtactatcga ttcacacaaa
      481 aaaccaacac acagatgtaa tgaaaataaa gatattttat tctcgaggtg ataattccac
      541 ttacgccagt cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg
      601 tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg
      661 tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg
      721 cggacctggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga
      781 ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg
      841 ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa
      901 tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag tattccatat
      961 atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat
     1021 ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg
     1081 gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg
     1141 acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc
     1201 caccattgac gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat
     1261 tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact
     1321 tggcaagtac attactattg gaagtacgcc agggtacatt ggcagtactc ccattgacgt
     1381 caatggcggt aaatggcccg cgatgtctgc caagtacatc cccattgacg tcaatgggga
     1441 ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg
     1501 gaggtctata taagcaatgc tcgtttaggg aacggggatc atcgaaaagc tttcatttag
     1561 gtgacactat agaatacaag ctacttgttc tatggagaca gacacactcc tgctatgggt
     1621 actgctgctc tgggttccag gttccactgg tgacgaacaa aaactcatct cagaagagga
     1681 tctgaatgct gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt
     1741 taaggtggtg gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat
     1801 catcctcatc atgctttggc agaagaagcc acgtggtggc tctggaggac tcgagaagtc
     1861 catgtccagc atggtgagcg atactagctg taccttccca tcatctgacg gaatcttctg
     1921 gaagcactgg attcagacta aggacggcca gtgtggcagc ccactggtga gcacacgaga
     1981 cggattcatc gtggggattc acagcgcctc caactttaca aacaccaata actatttcac
     2041 ctcagtgcca aagaacttta tggagctgct gaccaaccag gaggcccagc agtgggtgag
     2101 cgggtggcgc ctgaacgccg attccgtgct gtggggcggg cacaaggtgt ttatgaccgc
     2161 atgcggtggc ggtggctctg gaggtggtgg gtccggagga ggaggtggcg gttccggcgg
     2221 aggtgggtct ggtggaggtg gatctggagg tggtggatca ggaggaggag gttcaggcgg
     2281 ccgcgagttt ctggcaacca cactggaacg gatcgagaaa aatttcgtga ttactgatcc
     2341 gagactgcct gacaacccaa tcatttttgc gagcgattcc ttcctgcaac tgacagaata
     2401 ttctcgggaa gagatcctgg ggcgcaattg ccgttttctg caaggacccg agacagaccg
     2461 tgccactgtt cggaaaatca gagatgctat tgacaaccag actgaagtga ccgttcagct
     2521 gatcaattat accaagagcg gcaagaagtt ctggaacgtg ttccacctgc aaccgatgcg
     2581 cgattataag ggcgacgtcc agtacttcat tggcgtgcag ctggatggca ccgaacgtct
     2641 tcatggcgcc gctgagcgtg aggcggtctg cctgatcaaa aagacagcct ttcagattgc
     2701 tggatccatg cttcaacttc ctcctcttga acgtcttact ctcatgttgc agctaccacc
     2761 attagagaga ctaacactga tgggcgagag cctgttcaag ggacctaggg actacaaccc
     2821 tatcagtagc acaatttgtc acctgaccaa cgagagtgat ggccacacaa caagcctgta
     2881 cggcatcggg ttcggaccct ttatcatcac caacaagcac ctgttcaggc ggaataatgg
     2941 cactctgctg gtgcagagcc tgcacggggt gttcaaagtg aagaacacaa ccactctgca
     3001 gcagcacctg atcgatgggc gggatatgat catcattagg atgcccaagg acttcccccc
     3061 ttttcctcag aaactgaagt tccgagagcc ccagagagag gagagaatct gtctggtgac
     3121 cacaaacttt cagaccgagt ttctggcaac cacactggaa cggatcgaga aaaatttcgt
     3181 gattactgat ccgagactgc ctgacaaccc aatcattttt gcgagcgatt ccttcctgca
     3241 gctgacagaa tattctcggg aagagatcct ggggcgcaat tgccgttttc tgcagggacc
     3301 cgagacagac cgtgccactg ttcggaaaat cagagatgct attgacaacc agactgaagt
     3361 gaccgttcag ctgatcaatt ataccaagag cggcaagaag ttctggaacg tgttccacct
     3421 gcagccgatg cgcgattata agggcgacgt ccagtacttc attggcgtgc agctggatgg
     3481 caccgaacgt cttcatggcg ccgctgagcg tgaggcggtc tgcctgatca aaaagacagc
     3541 ctttcagatt gctgagaacc tgtacttcca gggctctaga ctggacaaga gcaaagtcat
     3601 aaactctgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact
     3661 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     3721 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact cctgccccct
     3781 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     3841 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     3901 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     3961 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     4021 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     4081 gaaacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     4141 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     4201 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     4261 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaaat
     4321 ctccagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4381 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4501 gcattttttt cactgccccg agcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4561 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4621 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5101 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5341 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5401 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5461 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5521 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5581 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5641 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5701 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5761 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5821 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5881 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5941 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6001 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6061 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6121 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6181 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6241 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6301 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6361 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6421 cgcacatttc cccgaaaagt gccacctgac gtcggcagtg aaaaaaatgc tttatttgtg
     6481 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     6541 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     6601 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagcc gcggcgtcgg
     6661 tggtgccggc ggggggcgcc aggtcgcagg cggtgtaggg ctccaggcag gcggcgaagg
     6721 ccatgacgtg cgctatgaag gtctgctcct gcacgccgtg aaccaggtgc gcctgcgggc
     6781 cgcgcgcgaa caccgccacg tcctcgcctg cgtgggtctc ttcgtccagg ggcactgctg
     6841 actgctgccg atactcgggg ctcccgctct cgctctcggt aacatccggc cgggcgccgt
     6901 ccttgagcac atagcctgga ccgtttccgt ataggaggac cgtgtaggcc ttcctgtccc
     6961 gggccttgcc aggggccagc ccgaagatgg agctccctcg cagggggtag cctccgaagg
     7021 agaagacgtg ggagtggtcg gcagtgacga ggctcagcgt gtcctcctcg ctggtgagct
     7081 ggcccgccct ctcaatggcg tcgtcgaaca tgatcgtctc agtcagtgcc cggtaagccc
     7141 tgctttcatg atgaccatgg tcgatgcgac caccctccac gaagaggaag aagccgcggg
     7201 ggttcctgct cagcaggcgc agggcagcct ctgtcatctc catcagggag gggtccagtg
     7261 tggagtctcg gtggatctcg tatttcatgt ctccaggctc aaagagaccc atgagatggg
     7321 tcacagacgg gtccagggaa gcctgcatga gctcagtgcg gttccacaca taccgggcac
     7381 cctggcgctt cgccagccat tcctgcacca gattcttccc gtccagcctg gtcccacctt
     7441 ggctgtagtc atctgggtac tcagggtctg gggttcccat gcgaaacatg tactttcggc
     7501 ctccacctag gatcacgtca atgtccatgt tggagatgag ctgcgtagcg atgtcctggc
     7561 acccctcctg gcgcgccgag gcaggcacgt cggcgtccga gtaccagttg cggttcaccg
     7621 tgtgggcgta ggtgccggct ggcgaggcgt gctgcactcg tgtggtggtt accactccca
     7681 ctgacttccc tgctttcttg gcccgattca tcacggagat gacctcgttg ccgcgtgtcg
     7741 tgttgcactg gttaaagcgg gcggctgcac tcaagccaat ggtctggaag ttgcccttga
     7801 ccccgcacag gtaggccgtg gctgtggctc cactgtctgg cacatgtttg tctacattgt
     7861 atgtcttgga cagagccaca tatgggaagc ggtccatggc caggggtatc tcaggcccca
     7921 gtttgtcctt cttctgccct tttaggatcc tggcagctgt caccgtagac acccccatcc
     7981 catcgcccag gaagatgatg aggttcttgg cggctgtctg tgcaggctgc agcttcttgg
     8041 cggcacccag ggcctcggct gcctcgcggt tccagaagtc cgggttctcc tcctcaactg
     8101 ggatgatgcc cagggagagc tgtagcctca ggcccagcag cagcagcagc agcagcatgg
     8161 tgggcgaatt cttta
//
