LOCUS       sequence-415579-       15555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15555)
  AUTHORS   Vanegas KG, Rendsvig JKH, Jarczynska ZD, Cortes MVCB, van Esch AP, 
            Morera-Gomez M, Contesini FJ, Mortensen UH
  TITLE     A Mad7 System for Genetic Engineering of Filamentous Fungi.
  JOURNAL   J Fungi (Basel). 2022 Dec 22;9(1):16. doi: 10.3390/jof9010016.
  PUBMED    36675838
REFERENCE   2  (bases 1 to 15555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2022-12-22"; volume: "9(1):16. doi"; pages: " 
            10.3390/jof9010016"
COMMENT     Sequence Label: pDIV303
FEATURES             Location/Qualifiers
     source          1..15555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      1590..6840
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             11815..11835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12675..14321
                     /codon_start=1
                     /gene="amdS"
                     /product="acetamidase from Aspergillus nidulans"
                     /label=amdS
                     /note="confers ability to process acetamide for selection 
                     in fungal cells"
                     /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
                     SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
                     RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
                     AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
                     TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT
                     KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
                     TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
                     NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
                     RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
                     AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
     rep_origin      complement(14844..15432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14924..14943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15554..15555,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt tggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa atatgggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
     1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
     1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
     1441 tagtcaccaa ctagtctatc aagactcaca gggtacattg ctgagaccaa ctgaccagag
     1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
     1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
     1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
     1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
     1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
     1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
     1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
     1921 aaaacaagac agccacatat tagggagact agttagaagc tagttccagg actaggaaaa
     1981 taaaagacaa tgataccaca gtctagttga caactagata gattctagat tgaggccaaa
     2041 gtcyctgaga tccaggttag ttgcaactaa tactagttag tatctagtct cctataactc
     2101 tgaagctaga ataacttact actattatcc tcaccactgt tcagctgcgc aaacggagtg
     2161 attgcaaggt gttcagagac tagttattga ctagtcagtg actagcaata actaacaagg
     2221 tattaaccta ccatgtctgc catcaccctg cacttcctcg ggctcagcag ccttttcctc
     2281 ctcattttca tgctcatttt ccttgtttaa gactgtgact agtcaaagac tagtccagaa
     2341 ccacaaagga gaaatgtctt accactttct tcattgcttg tctcttttgc attatccatg
     2401 tctgcaacta gttagagtct agttagtgac tagtccgacg aggacttgct tgtctccgga
     2461 ttgttggagg aactctccag ggcctcaaga tccacaacag agccttctag aagactggtc
     2521 aataactagt tggtctttgt ctgagtctga cttacgaggt tgcatactcg ctccctttgc
     2581 ctcgtcaatc gatgagaaaa agcgccaaaa ctcgcaatat ggctttgaac cacacggtgc
     2641 tgagactagt tagaatctag tcccaaacta gcttkgatag cttacctttg ccctttgcgt
     2701 tgcgacaggt cttgcagggt atggttcctt tctcaccagc tgatttagct gccttgctac
     2761 cctcacggcg gatctgcata aagagtggct agaggttata aattagcact gatcctaggt
     2821 acggggctga atgtaacttg ccyttccttt ctcatcgcgc ggcaagacag gcttgctcaa
     2881 attcctacca gtcacagggg tatgcacggc gtacggacca cttgaactag tcacagatta
     2941 gttagcaact agtctgcatt gaatggctgt acttacgggc cctcgccatt gtcctgatca
     3001 tttccagctt caccctcgtt gctgcaaagt agttagtgac tagtcaagga ctagttgaaa
     3061 tgggagaaga aactcacgaa ttctcgacac ccttagtatt gtggtccttg gacttggtgc
     3121 tgctatatat tagctaatac actagttaga ctcacagaaa cttacgcagc tcgcttgcgc
     3181 ttcttggtag gagtcggggt tgggagaaca gtgccttcaa acaagccttc ataccatgct
     3241 acttgactag tcagggacta gtcaccaagt aatctagata ggacttgcct ttggcctcca
     3301 tcagttcctt catagtggga ggtccattgt gcaatgtaaa ctccatgccg tgggagttct
     3361 tgtccttcaa gtgcttgacc aatatgtttc tgttggcaga gggaacctgt caactagtta
     3421 ataactagtc agaaactagt atagcagtag actcactgta cgcttgaggc atcccttcac
     3481 tcggcagtag acttcatatg gatggatatc aggcacgcca ttgtcgtcct gtggactagt
     3541 cagtaactag gcttaaagct agtcgggtcg gcttactatc ttgaaatccg gcagcgtaag
     3601 ctccccgtcc ttaactgcct cgagatagtg acagtactct ggggactttc ggagatcgtt
     3661 atcgcgaatg ctcggcatac taatcgttga ctagtcttgg actagtcccg agcaaaaagg
     3721 attggaggag gaggaggaag gtgagagtga gacaaagagc gaaataagag cttcaaaggc
     3781 tatctctaag cagtatgaag gttaagtatc tagttcttga ctagatttaa aagagatttc
     3841 aactagttat gtacctggag tttggatata ggaatgtgtt gtggtaacga aatgtaaggg
     3901 ggaggaaaga aaaagtcggt caagaggtaa ctctaagtcg gccattcctt tttgggaggc
     3961 gctaaccata aacggcatgg tcgacttaga gttagctcag ggaatttagg gagttatctg
     4021 cgaccaccga ggaacggcgg aatgccaaag aatcccgatg gagctctagc tggcggttga
     4081 caaccccacc ttttggcgtt tctgcggcgt tgcaggcggg actggatact tcgtagaacc
     4141 agaaaggcaa ggcagaacgc gctcagcaag agtgttggaa gtgatagcat gatgtgcctt
     4201 gttaactagg tcaaaatctg cagtatgctt gatgttatcc aaagtgtgag agaggaaggt
     4261 ccaaacatac acgattggga gagggcctag gtataagagt ttttgagtag aacgcatgtg
     4321 agcccagcca tctcgaggag attaaacacg ggccggcatt tgatggctat gttagtaccc
     4381 caatggaaac ggtgagagtc cagtggtcgc agataactcc ctaaattccc tgagctaact
     4441 ctaagtcgac catgccgttt atggttagcg cctcccaaaa aggaatggcc gacttagagt
     4501 tacctcttga ccgacttttt ctttcctccc ccttacattt cgttaccaca acacattcct
     4561 atatccaaac tccaggtaca taactagtcg aaatctcttt taaatctagt caagaactag
     4621 atacttaacc ttcatactgc ttagagatag cctttgaagc tcttatttcg ctctttgtct
     4681 cactctcacc ttcctcctcc tcctccaatc ctttttgctc gggactagtc caagactagt
     4741 caacgattag tatgccgagc attcgcgata acgatctccg aaagtcccca gagtactgtc
     4801 actatctcga ggcagttaag gacggggagc ttacgctgcc ggatttcaag atagtaagcc
     4861 gacccgacta gctttaagcc tagttactga ctagtccaca ggacgacaat ggcgtgcctg
     4921 atatccatcc atatgaagtc tactgccgag tgaagggatg cctcaagcgt acagtgagtc
     4981 tactgctata ctagtttctg actagttatt aactagttga caggttccct ctgccaacag
     5041 aaacatattg gtcaagcact tgaaggacaa gaactcccac ggcatggagt ttacattgca
     5101 caatggacct cccactatga aggaactgat ggaggccaaa ggcaagtcct atctagatta
     5161 cttggtgact agtccctgac tagtcaagta gcatggtatg aaggcttgtt tgaaggcact
     5221 gttctcccaa ccccgactcc taccaagaag cgcaagcgag ctgcgtaagt ttctgtgagt
     5281 ctaactagtg tattagctaa tatatagcag caccaagtcc aaggaccaca atactaaggg
     5341 tgtcgagaat tcgtgagttt cttctcccat ttcaactagt ccttgactag tcactaacta
     5401 ctttgcagca acgagggtga agctggaaat gatcaggaca atggcgaggg cccgtaagta
     5461 cagccattca atgcagacta gttgctaact aatctgtgac tagttcaagt ggtccgtacg
     5521 ccgtgcatac ccctgtgact ggtaggaatt tgagcaagcc tgtcttgccg cgcgatgaga
     5581 aaggaarggc aagttacatt cagccccgta cctaggatca gtgctaattt ataacctcta
     5641 gccactcttt atgcagatcc gccgtgaggg tagcaaggca gctaaatcag ctggtgagaa
     5701 aggaaccata ccctgcaaga cctgtcgcaa cgcaaagggc aaaggtaagc tatcmaagct
     5761 agtttgggac tagattctaa ctagtctcag caccgtgtgg ttcaaagcca tattgcgagt
     5821 tttggcgctt tttctcatcg attgacgagg caaagggagc gagtatgcaa cctcgtaagt
     5881 cagactcaga caaagaccaa ctagttattg accagtcttc tagaaggctc tgttgtggat
     5941 cttgaggccc tggagagttc ctccaacaat ccggagacaa gcaagtcctc gtcggactag
     6001 tcactaacta gactctaact agttgcagac atggataatg caaaagagac aagcaatgaa
     6061 gaaagtggta agacatttct cctttgtggt tctggactag tctttgacta gtcacagtct
     6121 taaacaagga aaatgagcat gaaaatgagg aggaaaaggc tgctgagccc gaggaagtgc
     6181 agggtgatgg cagacatggt aggttaatac cttgttagtt attgctagtc actgactagt
     6241 caataactag tctctgaaca ccttgcaatc actccgtttg cgcagctgaa cagtggtgag
     6301 gataatagta gtaagttatt ctagcttcag agttatagga gactagatac taactagtat
     6361 tagttgcaac taacctggat ctcagrgact ttggcctcaa tctagaatct atctagttgt
     6421 caactagact gtggtatcat tgtcttttat tttcctagtc ctggaactag cttctaacta
     6481 gtctccctaa tatgtggctg tcttgttttt tttttttngt ttccctaccc ggatatctag
     6541 tccccttcta ggttctgtta acctctcggg ctctgattta gtttaacgca aacctgagat
     6601 tagtttctaa ctagtctcta ggttttctat ccacctttaa ttgtaataat aaatacaagc
     6661 aacgtttata cgtcaaaagc atttataaac ttttacccta aagtagcttg cttgtgtgtt
     6721 tagtttataa ttagtctctt attaatttga tgtaggtaag cccgccacaa atatatattt
     6781 ttaacaagat accgtggaaa aacttcgtgc tatcacaaaa cagtatacaa aaaataagct
     6841 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
     6901 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
     6961 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
     7021 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
     7081 catggtcata gctggctgag ggtttaatgc tgagggttta attaagacct cagccgagac
     7141 agcagaatca ccgcccaagt taagcctttg tgctgatcat gctctcgaac gggccaagtt
     7201 cgggaaaagc aaaggagcgt ttagtgaggg gcaatttgac tcacctccca ggcaacagat
     7261 gaggggggca aaaagaaaga aattttcgtg agtcaatatg gattccgagc atcattttct
     7321 tgcggtctat cttgctacgt atgttgatct tgacgctgtg gatcaagcaa cgccactcgc
     7381 tcgctccatc gcaggctggt cgcagacaaa ttaaaaggcg gcaaactcgt acagccgcgg
     7441 ggttgtccgc tgcaaagtac agagtgataa aagccgccat gcgaccatca acgcgttgat
     7501 gcccagcttt ttcgatccga gaatccaccg tagaggcgat agcaagtaaa gaaaagctaa
     7561 acaaaaaaaa atttctgccc ctaagccatg aaaacgagat ggggtggagc agaaccaagg
     7621 aaagagtcgc gctgggctgc cgttccggaa ggtgttgtaa aggctcgacg cccaaggtgg
     7681 gagtctagga gaagaatttg catcgggagt ggggcgggtt acccctccat atccaatgac
     7741 agatatctac cagccaaggg tttgagcccg cccgcttagt cgtcgtcctc gcttgcccct
     7801 ccataaaagg atttcccctc cccctcccac aaaattttct ttcccttcct ctccttgtcc
     7861 gcttcagtac gtatatcttc ccttccctcg cttctctcct ccatccttct ttcatccatc
     7921 tcctgctaac ttctctgctc agcacctcta cgcattacta gccgtagtat ctgagcactt
     7981 ctccctttta tattccacaa aacataacac aaccttcatc atatgaacaa cggcaccaac
     8041 aacttccaga acttcatcgg catctccagc ctccaaaaga ccctccgcaa cgctctcatc
     8101 cccaccgaga ctacccagca gttcatcgtc aagaacggca tcatcaaaga ggatgagctg
     8161 cgcggcgaga accgccagat cctcaaggat atcatggatg attactaccg cggctttatc
     8221 tccgagactc tctcctccat cgatgatatc gattggacca gcctcttcga gaagatggaa
     8281 atccagctga agaacggcga taacaaggat accctgatca aagagcagac cgagtaccgc
     8341 aaggccatcc acaagaagtt cgccaacgac gatcgcttca agaatatgtt ctccgccaag
     8401 ctcatctccg atatcctgcc tgagttcgtc atccacaaca acaactactc cgcctccgag
     8461 aaagaggaaa agacccaggt catcaagctg ttctcccgct tcgccacctc cttcaaggat
     8521 tacttcaaga accgcgccaa ctgcttctcc gccgacgata tctcctccag ctcctgccac
     8581 cgcatcgtca acgataacgc cgagatcttc ttctccaacg ctctggtcta ccgccgcatc
     8641 gtgaagtccc tctccaacga tgacatcaac aagatctccg gcgatatgaa ggattccctc
     8701 aaagagatgt ccctcgagga aatctactcc tacgagaagt acggcgagtt cattacccaa
     8761 gagggcatct ccttctacaa cgatatctgc ggcaaggtca actccttcat gaacctctac
     8821 tgccagaaga acaaagagaa caagaacctg tacaagctcc agaagctcca caagcagatc
     8881 ctctgtatcg ccgataccag ctacgaggtc ccctacaagt tcgagtccga tgaggaagtc
     8941 taccagtccg tcaacggctt cctcgataac atcagctcca agcacatcgt cgagcgcctc
     9001 cgcaagatcg gcgacaacta caacggctac aacctcgata agatctacat cgtgtccaag
     9061 ttctacgagt ctgtctcgca aaagacctac cgcgattggg aaaccatcaa caccgctctc
     9121 gagatccatt acaacaacat cttgcccggc aacggcaagt ccaaggccga taaggtcaag
     9181 aaggccgtta agaacgatct gcagaagtcc atcaccgaga tcaacgagct ggtgtccaac
     9241 tacaagctct gctccgatga caacatcaag gccgaaacct acatccacga gatctcccac
     9301 atcctgaaca acttcgaggc ccaagagctg aagtacaacc ccgagatcca cctcgtcgag
     9361 agcgagctga aggcctccga gttgaagaac gtcctcgatg tcatcatgaa cgccttccac
     9421 tggtgctccg tgttcatgac cgaggaactc gtcgataagg ataacaactt ctacgccgag
     9481 ctggaagaaa tctacgatga gatctacccc gtcatctccc tgtacaacct ggtccgcaac
     9541 tacgtcaccc agaagcctta ctccaccaag aagatcaagc tcaacttcgg catccccact
     9601 ctcgccgatg gttggtccaa gtccaaagag tactccaaca acgccatcat cctcatgcgc
     9661 gataacctgt actacctcgg catcttcaac gccaagaaca agcccgataa gaagattatc
     9721 gagggcaaca ccagcgagaa caagggcgat tacaagaaga tgatctacaa cctgctgcct
     9781 ggtcctaaca agatgatccc caaggtgttc ctgtcctcca agaccggcgt cgagacttac
     9841 aagccctccg cttacatcct cgaaggctac aagcagaaca agcacattaa gtccagcaag
     9901 gatttcgata tcaccttctg ccacgatctg atcgactatt tcaagaactg cattgccatc
     9961 catcctgagt ggaagaactt cggcttcgat ttctccgata cctccaccta cgaggatatc
    10021 tccggcttct accgcgaggt cgagctgcag ggctacaaga tcgactggac ctacatcagc
    10081 gagaaggata tcgatctcct gcaagagaag ggccagctct acctcttcca gatctataac
    10141 aaagatttct ccaagaagtc caccggcaac gacaacctcc acaccatgta cctcaagaac
    10201 ctcttcagcg aggaaaacct caaggacatc gtcctcaagc tgaacggcga ggctgaaatc
    10261 ttcttcagaa agtcctccat caagaacccc atcatccata agaagggctc catcctcgtc
    10321 aaccgcacct acgaagccga ggaaaaggat cagttcggca acatccagat cgtccgcaag
    10381 aacatccccg agaacatcta ccaagagttg tacaagtact tcaacgacaa gtccgacaaa
    10441 gagctgtccg acgaggccgc aaagctcaag aacgtcgtcg gccaccatga ggccgccacc
    10501 aacatcgtta aggattaccg ctatacctac gacaagtatt tcctgcacat gcccatcacc
    10561 atcaacttca aggccaacaa gaccggtttc atcaacgatc gcatcctcca gtatatcgcc
    10621 aaagagaagg atctccacgt catcggtatc gatcgcggcg agcgcaacct catctacgtg
    10681 tccgtcatcg atacctgcgg taacatcgtg gaacagaagt ccttcaacat cgtcaatggc
    10741 tacgattacc agatcaagtt gaagcagcaa gagggcgctc gccagatcgc tcgcaaagag
    10801 tggaaagaga tcggcaagat caaagaaatc aaagagggct acctgagcct ggtgatccat
    10861 gagatttcca agatggtgat caagtacaac gctatcattg ccatggaaga tctgtcctac
    10921 ggtttcaaga agggccgctt caaggtcgaa cgccaggtct accaaaagtt cgaaaccatg
    10981 ctgatcaaca agttgaacta cctcgtcttt aaggacatct ccattaccga gaacggcggc
    11041 ctcctcaagg gctaccagct cacctacatt cccgataagc tgaaaaacgt cggccatcag
    11101 tgcggctgca tcttctacgt ccctgccgcc tacacctcca agatcgatcc caccaccggc
    11161 ttcgtcaaca tcttcaagtt caaggatctc accgtcgatg ccaagcgcga attcatcaag
    11221 aagtttgatt ccatccgcta cgattccgag aagaatctct tctgtttcac cttcgactac
    11281 aacaatttca tcactcagaa caccgtcatg tccaagtcgt cctggtccgt ctacacctac
    11341 ggcgtccgca tcaagcgccg cttcgttaac ggccgcttct cgaacgagtc cgacaccatc
    11401 gacatcacca aggatatgga aaagacactc gagatgaccg atatcaactg gcgcgacggc
    11461 cacgacctcc gccaggacat catcgactac gagatcgtcc agcatatctt cgaaatcttc
    11521 cgcctcaccg tccagatgcg caactccctg tccgagttgg aggatcgcga ctacgatcgc
    11581 ctcatctccc ctgtcctcaa cgagaacaac attttctacg atagcgccaa ggccggcgac
    11641 gccctgccta aggatgccga cgccaacggc gcctactgta tcgctctcaa gggcctgtac
    11701 gagatcaagc aaatcaccga aaactggaaa gaggacggaa agttcagccg cgacaagctg
    11761 aagatctcca acaaggattg gttcgatttc atccaaaaca agcgctacct gcctcccaag
    11821 aagaagcgca aggtctgaag cggacattcg atttatgccg ttatgacttc cttaaaaaag
    11881 cctttacgaa tgaaagaaat ggaattagac ttgttatgta gttgattcta caatggatta
    11941 tgattcctga acttcaaatc cgctgttcat tattaatctc agctcttccc gtaaagccaa
    12001 tgttgaaact attcgtaaat gtacctcgtt ttgcgtgtac cttgcttatc acgtgatatt
    12061 acatgacctg gacagagttc tgcgcgaaag tcataacgta aatcccgggc ggtaggtgcg
    12121 tcccgggcgg aaggtagttt tctcgtccac cccaacgcgt ttatcaacct caactttcaa
    12181 caaccatcat gccaccaaaa gcgcgtaaaa caaagcgaga tttgattgag caagagggca
    12241 ggatccaatg cgcgattcaa gacattaaaa atggaaaatt tcaaaaaatt gcgcccgcag
    12301 cgcgtgcata caaaattcat cccaatacat taagacctca gcgctagtgg aggtcaacac
    12361 atcaatgcct attttggttt agtcgtccag gcggtgagca caaaatttgt gtcgtttgac
    12421 aagatggttc atttaggcaa ctggtcagat cagccccact tgtagcagta gcggcggcgc
    12481 tcgaagtgtg actcttatta gcagacagga acgaggacat tattatcatc tgctgcttgg
    12541 tgcacgataa cttggtgcgt ttgtcaagca aggtaagtgg acgacccggt cataccttct
    12601 taagttcgcc cttcctccct ttatttcaga ttcaatctga cttacctatt ctacccaagc
    12661 atccaaaaac aaccatgcct caatcctggg aagaactggc cgctgataag cgcgcccgcc
    12721 tcgcaaaaac catccctgat gaatggaaag tccagacgct gcctgcggaa gacagcgtta
    12781 ttgatttccc aaagaaatcg gggatccttt cagaggccga actgaagatc acagaggcct
    12841 ccgctgcaga ccttgtgtcc aagctggcgg ccggagagtt gacctcggtg gaagttacgc
    12901 tagcattctg taaacgggca gcaatcgccc agcagttaac aaactgcgcc cacgagttct
    12961 tccctgacgc cgctctcgcg caggcaaggg aactcgatga atactacgca aagcacaaga
    13021 gacccgttgg tccactccat ggcctcccca tctctctcaa agaccagctt cgagtcaagg
    13081 gctacgaaac atcaatgggc tacatctcat ggctaaacaa gtacgacgaa ggggactcgg
    13141 ttctgacaac catgctccgc aaagccggtg ccgtcttcta cgtcaagacc tctgtcccgc
    13201 agaccctgat ggtctgcgag acagtcaaca acatcatcgg gcgcaccgtc aacccacgca
    13261 acaagaactg gtcgtgcggc ggcagttctg gtggtgaggg tgcgatcgtt gggattcgtg
    13321 gtggcgtcat cggtgtagga acggatatcg gtggctcgat tcgagtgccg gccgcgttca
    13381 acttcctgta cggtctaagg ccgagtcatg ggcggctgcc gtatgcaaag atggcgaaca
    13441 gcatggaggg tcaggagacg gtgcacagcg ttgtcgggcc gattacgcac tctgttgagg
    13501 acctccgcct cttcaccaaa tccgtcctcg gtcaggagcc atggaaatac gattccaagg
    13561 tcatccccat gccctggcgc cagtccgagt cggacattat tgcctccaag atcaagaacg
    13621 gcgggctcaa tatcggctac tacaacttcg acggcaatgt ccttccacac cctcctatcc
    13681 tgcgcggcgt ggaaaccacc gtcgccgcac tcgccaaagc cggtcacacc gtgaccccgt
    13741 ggacgccata caagcacgat ttcggccacg atctcatctc ccatatctac gcggctgacg
    13801 gcagcgccga cgtaatgcgc gatatcagtg catccggcga gccggcgatt ccaaatatca
    13861 aagacctact gaacccgaac atcaaagctg ttaacatgaa cgagctctgg gacacgcatc
    13921 tccagaagtg gaattaccag atggagtacc ttgagaaatg gcgggaggct gaagaaaagg
    13981 ccgggaagga actggacgcc atcatcgcgc cgattacgcc taccgctgcg gtacggcatg
    14041 accagttccg gtactatggg tatgcctctg tgatcaacct gctggatttc acgagcgtgg
    14101 ttgttccggt tacctttgcg gataagaaca tcgataagaa gaatgagagt ttcaaggcgg
    14161 ttagtgagct tgatgccctc gtgcaggaag agtatgatcc ggaggcgtac catggggcac
    14221 cggttgcagt gcaggttatc ggacggagac tcagtgaaga gaggacgttg gcgattgcag
    14281 aggaagtggg gaagttgctg ggaaatgtgg tgactccata gatcagtact ccgacgccga
    14341 ccaacaccgc cggtccgacg cggcccgacg ggtccgagga gcttgagatc cacttaacgt
    14401 tactgaaatc atcaaacagc ttgacgaatc tggatataag atcgttggtg tcgatgtcag
    14461 ctccggagtt gagacaaatg gtgttcagga tctcgataag atacgttcat ttgtccaagc
    14521 agcaaagagt gccttctagt gatttaatag ctccatgtca acaagaataa aacgcgtttc
    14581 gggtttacct cttccagata cagctcatct gcaatgcatt aatgcattgg acctcgcaac
    14641 cctagtacgc ccttcaggct ccggcgaagc agaagaatag cttagcagag tctattttca
    14701 ttttcgggag acgagatcaa gcagatcaac ggtcgtcaag agacctacga gactgaggaa
    14761 tccgctcttg gctcattaag acctcagcga gcaaaaggcc agcaaaaggc caggaaccgt
    14821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    14881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    14941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    15001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    15061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    15121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    15181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    15241 acagagttct tgaagtggtg ggctaactac ggctacacta gaagaacagt atttggtatc
    15301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    15361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    15421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac
    15481 gcgcgcgtaa ctcacgttaa gggattttgg tcatgagctt gcgccgtccc gtcaagtcag
    15541 cgtaatgctc tgctt
//
