LOCUS       sequence-415595-       12345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal mEos 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12345)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos Cav2.2 pRK5
FEATURES             Location/Qualifiers
     source          1..12345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        44..423
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        424..627
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     578..598
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     624..648
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        858..876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     858..875
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     889..908
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             961..1638
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     regulatory      8675..8684
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8688..8822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8725..8744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8779..8798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        8891..9248
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8891..9082
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8914..8934)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9099..9234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9268..9285)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9268..9284)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9277..9299)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9497..9952
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9584..9603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9794..9815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9969..9988)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10088..10110
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10148..10166)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10234..10338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10339..10407,10408..11199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10557..10576)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11370..11958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11859..11878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12112..12129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12246..12267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12282..12287,12288..12305,12306..12312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12320..12336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(12325..12345,1..2)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(12344..12345,1..15)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(12344..12345,1..15)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggaaacagct atgacatgat tacgaattaa ttcgagctcg cccgacattg attattgact
       61 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      121 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      181 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      241 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      301 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      361 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      421 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      481 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      541 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      601 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      661 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
      721 gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga
      781 gtctataggc ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta
      841 tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt gcctttctct
      901 ccacaggtgt ccactcccag gtccaactgc acctcggttc tatcgattga attcggatcc
      961 atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg
     1021 caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg
     1081 gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca
     1141 ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag
     1201 cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt
     1261 tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt
     1321 tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag
     1381 ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat tcatatggct
     1441 ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag
     1501 gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc
     1561 catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg
     1621 cctgacaatg ccagacgagt ccgcttcggg gacgagctgg gcggccgcta tgggggcgcg
     1681 ggcggcgcgg agcgggcccg gggcggcggg gccggcgggg ctggcggccc gggccccggg
     1741 gggctgccgc cgggccagcg ggtcctctac aaacagtcga tcgcgcagcg cgcgcggacc
     1801 atggcgctgt acaaccccat cccggtcaag cagaactgct tcaccgtcaa ccgctcgctc
     1861 ttcgtcttca gcgaggacaa cgtcgtccgc aaatacgcca agcgcatcac cgagtggcct
     1921 ccgttcgagt acatgatcct ggccaccatc atcgccaact gcatcgtgct ggccctggag
     1981 cagcacctcc ctgacgggga caagacgcct atgtccgagc gcctggatga caccgagccc
     2041 tacttcattg ggatcttctg cttcgaggcg gggatcaaga tcctggccct gggctttgtt
     2101 ctgcacaagg gctcctatct gcggaacggc tggaacgtca tggacttcgt ggtagtcctc
     2161 acggggattc ttgccacagc cggaaccgac ttcgacctgc gaacgctgag ggctgtgcgt
     2221 gtgctaaggc ccctgaagct ggtgtctggg attccgagct tgcaggtggt gctcaagtcc
     2281 atcatgaagg ccatggtccc gctgctgcag atcgggctgc tgctcttctt cgccatcctc
     2341 atgtttgcca tcatcggcct cgagttctac atgggcaagt tccacaaggc ctgcttcccc
     2401 aacagcacag acccagatcc cgtgggtgac ttcccctgtg gcaaggaggc cccggcccgg
     2461 ctctgtgagg gagacacgga gtgccgggag tactgggccg gacccaactt tggtatcacc
     2521 aactttgaca acatcctgtt tgccatcttg accgtgttcc agtgcatcac catggagggc
     2581 tggactgaca tcctctacaa tacaaacgat gcagccggca acacgtggaa ctggctctac
     2641 ttcatccctc tcatcatcat cggctccttc ttcatgctca acctggtgct gggtgtgctc
     2701 tcaggagagt ttgctaagga gcgcgagaga gtggagaacc gccgcgcctt cctgaagctc
     2761 cggcggcagc agcagattga gcgggagctc aacgggtacc tggagtggat cttcaaggct
     2821 gaggaagtca tgctggctga ggaggacagg aacgcagagg agaagtcccc tctggatgca
     2881 gtgttgaaga gagcagctgc caagaagagc agaagcgacc tgatccaggc cgaggagggg
     2941 gagggccggc tcacaggcct ctgtgctccc ggttccccgt tcgcccgtgc cagcctgaaa
     3001 agcgggaaga cggagagctc gtcgtacttc cggaggaagg agaagatgtt ccgcttcttc
     3061 atccggcgca tggtgaaggc acagagcttc tactggacgg tgctgtgtgt ggtggcactg
     3121 aacacactgt gcgtggccat ggtgcactac aaccagccgc agcggctcac cacggcattg
     3181 tactttgcag agtttgtttt cctgggtctc ttcctcacag agatgtccct gaagatgtat
     3241 ggcctggggc ccaggagcta cttccggtcc tccttcaact gttttgactt tggggtcatc
     3301 gtgggcagta tctttgaagt ggtctgggct gctgtcaagc cagggacctc cttcgggatc
     3361 agtgttctgc gggctctgcg cttactgaga atcttcaagg tcaccaaata ctggaactcc
     3421 ctacggaacc tggtggtgtc tctgctcaac tccatgaagt ccatcatcag cctcctcttc
     3481 ctgctcttcc tgttcattgt tgtctttgct ctgctgggga tgcagctgtt tggaggccag
     3541 ttcaacttta aagacgagac cccaacgacc aactttgaca ccttccctgc tgctatcctc
     3601 accgtcttcc agatcctgac cggagaggac tggaatgcgg tcatgtacca cgggatcgag
     3661 tcgcaaggtg gcgtcagcag aggaatgttc tcctccttct acttcattgt cctcactctg
     3721 tttgggaact acaccctgct gaatgtcttc ctggccattg ctgtggacaa ccttgccaat
     3781 gctcaggagc tgaccaagga tgaagaggag atggaagaag cagccaatca gaaactcgcc
     3841 ttgcagaagg ccaaggaagt ggctgaagtc agccccatgt ctgctgcgaa catctccatt
     3901 gccgccaggc agcagaactc ggccaaagcg cgctcggtgt gggagcagcg agccagccag
     3961 ctgcggctgc agaacctgag ggccagctgc gaggcgctgt acagcgagat ggaccccgag
     4021 gagaggctgc gctacgccac cgcgcgccac ctgcggcccg acgtgaagac gcacctggac
     4081 cggccgctgg tggttgagcc cggccgcgac gctccgcggg gccccccggg ggggaagtcc
     4141 cggccggatg gctcggaggc ccccgagggt gcggatcccc cgcgcaggca tcaccggcac
     4201 cgagacaagg acaaggctcc cgccacggtc ccctcggcgg gggaacagga cagagccgaa
     4261 gctctgaggg cagagggcgg ggagctgggg ccccgcgagg agcgggggcg gccacgccgc
     4321 agccgcagca aggaggcccc gggcgccccg gaggtgcgca gcgatcgcgg ccgtggcccg
     4381 tgtcctgaag gtggtcggcg ccaccaccgg cgcgggtcac cggaggaggc agctgagagg
     4441 gagccccgcc gccaccgcgc gcaccggcac gggcccgacc cgggcaagga gggcccagcg
     4501 tccgggacca ggggcgagcg gcgcgcacga caccgcacgg gcccccgggc gtgcccccgg
     4561 gaggctgaga gcagcgagga gcctgcgcgg cggcaccggg ctcggcacaa ggcgccgccc
     4621 acccaggaga ccgcggagaa ggacaaggag gctgccgaga aggggggcga ggccacggag
     4681 gccgagaagg acaaggaggc tcggaaccac cagcccaagg agctgccctg tgacctggag
     4741 gccatcggca tgctgggagt gggcgctgtg catacgctgc ccagcacctg tctgcagaag
     4801 gtggaggagc agccagagga tgcggacaac cagcggaacg tcacccgcat gggtagtcag
     4861 cccccggaca ccagcaccac tgtgcacatt cctgtgacgc tgaccggccc ccccggggag
     4921 accacggtag tgcccagtgg aaacgtggac ctggaaagtc aagcagaggg gaagaaggag
     4981 gtggaaacca gcgatgtgat gaggagcggc cctcggccca ttgtcccgta cagttccatg
     5041 ttctgtttaa gcccaaccaa cctgctgcgc cgctgctgcc actacattgt gaccatgcga
     5101 tacttcgaga tggtcatcct cgtggtcatc gccctgagca gcatcgccct ggctgccgag
     5161 gaccccgtga gaacagactc tcccaggaat aatgctctga agtacatgga ctacatcttc
     5221 acaggcgtct tcaccttcga gatggtgata aagatgatcg atttgggact gctgctacac
     5281 cctggagcct atttccggga tctgtggaac attctggact tcattgtggt cagtggggcc
     5341 ctggtggcat ttgctttctc aggatccaaa gggaaagaca tcagcaccat caaatccctg
     5401 agagtcctgc gtgtgctgcg gccactcaag accatcaagc ggctgcccaa gctcaaggcc
     5461 gtgtttgact gtgtggtgaa ctcactgaaa aacgtcctca acatcctgat cgtctacatg
     5521 ctcttcatgt tcatctttgc cgtcattgcc gtgcagctct tcaaagggaa gttcttctac
     5581 tgcacggacg aatccaagga gctggagcgt gactgcaggg gtcagtattt ggattatgag
     5641 aaggaggaag tggaagctca accgaggcag tggaagaaat atgacttcca ctatgacaac
     5701 gtgctctggg ctctgctaac gctcttcacg gtgtccacag gggaagggtg gcccatggtg
     5761 ctgaaacact ctgtggatgc cacctacgag gagcagggcc cgagccccgg gtaccgcatg
     5821 gagctctcca tcttctacgt ggtctacttt gtggtctttc ccttcttctt tgtcaacatc
     5881 ttcgtggcct tgatcatcat caccttccag gagcaggggg acaaggtgat gtccgagtgc
     5941 agcctggaga agaacgagag ggcttgcatc gactttgcca tcagcgccag acccctgacg
     6001 cggtacatgc cccagaacaa gcagtcgttc cagtataaga cgtggacgtt cgtggtgtcc
     6061 ccaccttttg agtacttcat catggctatg atcgccctca acactgtggt gctgatgatg
     6121 aagttctacg atgcgccgta cgagtatgag ctaatgctga aatgcctgaa catcgtgttc
     6181 acgtccatgt tctccatgga gtgtgtgctg aagatcatcg ccttcggggt gctgaactat
     6241 ttcagagatg cctggaatgt ctttgacttt gtcacggtgt tgggaagtat tactgatatt
     6301 ttggtaacag agattgcgaa caacttcatc aacctcagct tcctgcgcct gttccgggcc
     6361 gcgcggctca tcaagctgct gcgccagggc tacaccatcc gcatcctgct ttggaccttc
     6421 gtgcagtcct tcaaggcgct gccctacgtg tgtctcctca tcgccatgct cttcttcatc
     6481 tacgccatca ttggcatgca ggtttttggg aacatcgccc tggacgatga caccagcatc
     6541 aacaggcaca ataacttccg gacgtttctg caggccttga tgttgctgtt caggagcgcc
     6601 acgggggagg cctggcacga gataatgctg tcgtgcctca gcagccgggc ctgcgacgag
     6661 cactccaatg ccagcgagtg cggcagcgac tttgcctact tttacttcgt ctccttcatc
     6721 ttcctctgct cctttctgat gttgaacctc tttgtggctg tgatcatgga caattttgag
     6781 taccttacac gggactcttc catcttaggg cctcaccacc tggatgagtt catccgggtc
     6841 tgggctgaat acgacccggc cgcgtgtggg cgcatcagtt acagtgacat gtttgagatg
     6901 ctgaagcaca tgtccccacc cctggggctg gggaagaagt gccctgctcg agtcgcgtac
     6961 aagcgcctgg tccgcatgaa catgcccatc tccagtgagg acatgacggt gcacttcaca
     7021 tccacgctga tggctctcat tcggacagcg ctggacatca agctggcacc agctgggacc
     7081 aagcagcacc agtgcgacgc agagctgagg aaggagattt cctgtgtgtg ggccaatctg
     7141 ccccagaaga ccctggactt actggtacca ccccataagc ctgacgagat gacggtgggc
     7201 aaagtctatg cagctctgat gatcttcgac ttctacaaac agaacaaaac cagcagagac
     7261 cagactcagc aagcccccgg aggcctgtcc cagctgggcc ccgtgtccct gttccacccg
     7321 ctgaaggcca cgctggagca gactcagccg gcgctgcgtg gagcccgggc tttcctgcgg
     7381 cagaagagtt ctgcctccct gagcaacggc ggggccgtac aaacccagga gagcggcatc
     7441 aaggaatcgg tttcttgggg cactcagagg acccaggatg tgctttgcga ggccagggca
     7501 cccctggagc ggggccactc agctgagatc ccagtcgggc agcctgggac actggctgta
     7561 gatgtccaga tgcagaacat gactctgagt ggccctgacg ccgagccgca gccagggctg
     7621 gagagccagg gccgagccgc ctccatgccc cgcctggccg cagaaacaca gcccgcgcct
     7681 gacgccagcc ccatgaagcg ctccatctcc acgctggccc cgcgccccca cacggcccgg
     7741 ctcggcagca ccgcgctgga ccgcccagcc cccagccagg ccccgcacca tcaccaccac
     7801 cgctgccacc gccgcaggga caggaagcag cggtccctgg agaagggtcc cagcctgtct
     7861 gctgacacag atggggcccc cgacagcacg gtggggccag gactgcccac gggggagggc
     7921 ccccccggct gcaggcggga gcgggagcgc aggcaggagc ggggccggtc ccaggagcgg
     7981 aggcagccct cgtcctcctc ctcggagaag catcgcttct actcctgtga ccgcttcggg
     8041 ggccgcgaac ccccacagcc caagccctcc ctcagtagtc accccacgtc gccgacggcc
     8101 gggcaggagc cgggacccca cccgcagggg agtggttcgg ttcacgggag ccccctgctg
     8161 tccacatctg gtgctagcac cccgggccgg gggcggaggc agctccccca gacccccttg
     8221 accccccgcc ccagcgtcac ctacaagacg gccaactcct cgcctgtcca cttcgccggg
     8281 gcaccgagcg gcctccccgc cttctccccc ggccggctca gccggggcct ctcagaacac
     8341 aacgccctgc tgcagaggga cccgctcagc cggcccctgg cgcccggctc gcgcatcggc
     8401 tccgacccct acctggggca gcggctggac agcgaggcgc ccgcccgtgc cctgcccgag
     8461 gacgcgcccg cctttgagga gaccgcggcc agcaactcgg gccgctcctc ccggacctcg
     8521 tacgtgtcct cactgacctc gcagcccccg ccgctgcgcc gcgtgcccaa cggctaccac
     8581 tgcactctgg ggctgggcgg gggcggccgg gcgcggcgcg gctgccacca ccccgaccgc
     8641 gaccgccggt gctagactag ttctagcaag cttggccgcc atggcccaac ttgtttattg
     8701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     8821 tcgggaatta attcggcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     8881 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     8941 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9001 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9061 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9121 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9181 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9241 tttgcaaaaa gctgttaaca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     9301 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     9361 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9421 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     9481 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     9721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     9841 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9901 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     9961 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
    10021 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    10081 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
    10141 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
    10201 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    10261 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    10321 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    10381 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    10441 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    10501 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    10561 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
    10621 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    10681 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    10741 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    10801 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10861 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10921 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10981 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    11041 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    11101 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    11161 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    11221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    11281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    11341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    11401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    11461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    11521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    11581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    11641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    11701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    11761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    11821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    11881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    11941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    12001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    12061 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    12121 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    12181 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    12241 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    12301 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acaca
//
