LOCUS       sequence-415597-        8911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of the N-terminus, Domain I, Domain II and the II-III 
            loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin 
            AC
  TITLE     Dominant-negative synthesis suppression of voltage-gated calcium 
            channel Cav2.2 induced by truncated constructs.
  JOURNAL   J Neurosci. 2001 Nov 1;21(21):8495-504.
  PUBMED    11606638
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2001-11-1"; volume: "21(21)"; pages: "8495-504"
COMMENT     Sequence Label: Cav2.2 DomI + DomII pcDNA3
FEATURES             Location/Qualifiers
     source          1..8911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             98..892
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(152..171)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     762..781
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1066..1187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1103..1122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1157..1176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1236..1252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1236..1252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1249..1271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1260..1276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1284..1290,1291..1308,1309..1314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1329..1350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1467..1484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1638..2226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1718..1737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2397..3188,3189..3257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3020..3039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3258..3362)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3437..3456)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3628..4007
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4008..4211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4162..4182
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4256..4275
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4256..4274
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7857..7875)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7858..7875)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7895..7912)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7901..8125
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8171..8599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8258..8277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8468..8489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8608..8628)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8613..8911,1..31)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8793..8911,1..17)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8855..8874
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
       61 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      121 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      181 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      241 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      301 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      361 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      421 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      481 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      541 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      601 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
      661 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
      721 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
      781 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
      841 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
      901 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
      961 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1021 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     1081 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1141 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     1201 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1261 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1321 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1381 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1441 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2041 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2701 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3001 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3361 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     3421 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     3481 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     3541 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     3601 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     3661 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3721 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3781 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3841 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3901 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3961 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     4021 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4081 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4141 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4201 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     4261 cgactcacta tagggagacc caagcttggt accgagctcg gatccactag taacggccgc
     4321 cagtgtgctg gaattcctcg agggtaccgg gccccccctc gaggtcgaag cttgatcatg
     4381 gtccgcttcg gggacgagct gggcggccgc tatgggggcg cgggcggcgc ggagcgggcc
     4441 cggggcggcg gggccggcgg ggctggcggc ccgggccccg gggggctgcc gccgggccag
     4501 cgggtcctct acaaacagtc gatcgcgcag cgcgcgcgga ccatggcgct gtacaacccc
     4561 atcccggtca agcagaactg cttcaccgtc aaccgctcgc tcttcgtctt cagcgaggac
     4621 aacgtcgtcc gcaaatacgc caagcgcatc accgagtggc ctccgttcga gtacatgatc
     4681 ctggccacca tcatcgccaa ctgcatcgtg ctggccctgg agcagcacct ccctgacggg
     4741 gacaagacgc ctatgtccga gcgcctggat gacaccgagc cctacttcat tgggatcttc
     4801 tgcttcgagg cggggatcaa gatcctggcc ctgggctttg ttctgcacaa gggctcctat
     4861 ctgcggaacg gctggaacgt catggacttc gtggtagtcc tcacggggat tcttgccaca
     4921 gccggaaccg acttcgacct gcgaacgctg agggctgtgc gtgtgctaag gcccctgaag
     4981 ctggtgtctg ggattccgag cttgcaggtg gtgctcaagt ccatcatgaa ggccatggtc
     5041 ccgctgctgc agatcgggct gctgctcttc ttcgccatcc tcatgtttgc catcatcggc
     5101 ctcgagttct acatgggcaa gttccacaag gcctgcttcc ccaacagcac agacccagat
     5161 cccgtgggtg acttcccctg tggcaaggag gccccggccc ggctctgtga gggagacacg
     5221 gagtgccggg agtactgggc cggacccaac tttggtatca ccaactttga caacatcctg
     5281 tttgccatct tgaccgtgtt ccagtgcatc accatggagg gctggactga catcctctac
     5341 aatacaaacg atgcagccgg caacacgtgg aactggctct acttcatccc tctcatcatc
     5401 atcggctcct tcttcatgct caacctggtg ctgggtgtgc tctcaggaga gtttgctaag
     5461 gagcgcgaga gagtggagaa ccgccgcgcc ttcctgaagc tccggcggca gcagcagatt
     5521 gagcgggagc tcaacgggta cctggagtgg atcttcaagg ctgaggaagt catgctggct
     5581 gaggaggaca ggaacgcaga ggagaagtcc cctctggatg cagtgttgaa gagagcagct
     5641 gccaagaaga gcagaagcga cctgatccag gccgaggagg gggagggccg gctcacaggc
     5701 ctctgtgctc ccggttcccc gttcgcccgt gccagcctga aaagcgggaa gacggagagc
     5761 tcgtcgtact tccggaggaa ggagaagatg ttccgcttct tcatccggcg catggtgaag
     5821 gcacagagct tctactggac ggtgctgtgt gtggtggcac tgaacacact gtgcgtggcc
     5881 atggtgcact acaaccagcc gcagcggctc accacggcat tgtactttgc agagtttgtt
     5941 ttcctgggtc tcttcctcac agagatgtcc ctgaagatgt atggcctggg gcccaggagc
     6001 tacttccggt cctccttcaa ctgttttgac tttggggtca tcgtgggcag tatctttgaa
     6061 gtggtctggg ctgctgtcaa gccagggacc tccttcggga tcagtgttct gcgggctctg
     6121 cgcttactga gaatcttcaa ggtcaccaaa tactggaact ccctacggaa cctggtggtg
     6181 tctctgctca actccatgaa gtccatcatc agcctcctct tcctgctctt cctgttcatt
     6241 gttgtctttg ctctgctggg gatgcagctg tttggaggcc agttcaactt taaagacgag
     6301 accccaacga ccaactttga caccttccct gctgctatcc tcaccgtctt ccagatcctg
     6361 accggagagg actggaatgc ggtcatgtac cacgggatcg agtcgcaagg tggcgtcagc
     6421 agaggaatgt tctcctcctt ctacttcatt gtcctcactc tgtttgggaa ctacaccctg
     6481 ctgaatgtct tcctggccat tgctgtggac aaccttgcca atgctcagga gctgaccaag
     6541 gatgaagagg agatggaaga agcagccaat cagaaactcg ccttgcagaa ggccaaggaa
     6601 gtggctgaag tcagccccat gtctgctgcg aacatctcca ttgccgccag gcagcagaac
     6661 tcggccaaag cgcgctcggt gtgggagcag cgagccagcc agctgcggct gcagaacctg
     6721 agggccagct gcgaggcgct gtacagcgag atggaccccg aggagaggct gcgctacgcc
     6781 accgcgcgcc acctgcggcc cgacgtgaag acgcacctgg accggccgct ggtggttgag
     6841 cccggccgcg acgctccgcg gggccccccg ggggggaagt cccggccgga tggctcggag
     6901 gcccccgagg gtgcggatcc cccgcgcagg catcaccggc accgagacaa ggacaaggct
     6961 cccgccacgg tcccctcggc gggggaacag gacagagccg aagctctgag ggcagagggc
     7021 ggggagctgg ggccccgcga ggagcggggg cggccacgcc gcagccgcag caaggaggcc
     7081 ccgggcgccc cggaggtgcg cagcgatcgc ggccgtggcc cgtgtcctga aggtggtcgg
     7141 cgccaccacc ggcgcgggtc accggaggag gcagctgaga gggagccccg ccgccaccgc
     7201 gcgcaccggc acgggcccga cccgggcaag gagggcccag cgtccgggac caggggcgag
     7261 cggcgcgcac gacaccgcac gggcccccgg gcgtgccccc gggaggctga gagcagcgag
     7321 gagcctgcgc ggcggcaccg ggctcggcac aaggcgccgc ccacccagga gaccgcggag
     7381 aaggacaagg aggctgccga gaaggggggc gaggccacgg aggccgagaa ggacaaggag
     7441 gctcggaacc accagcccaa ggagctgccc tgtgacctgg aggccatcgg catgctggga
     7501 gtgggcgctg tgcatacgct gcccagcacc tgtctgcaga aggtggagga gcagccagag
     7561 gatgcggaca accagcggaa cgtcacccgc atgggtagtc agcccccgga caccagcacc
     7621 actgtgcaca ttcctgtgac gctgaccggc ccccccgggg agaccacggt agtgcccagt
     7681 ggaaacgtgg acctggaaag tcaagcagag gggaagaagg aggtggaaac cagcgatgtg
     7741 atgaggagcg gccctcggcc cattgtcccg tacagttcca tgttctgttt aagcccaacc
     7801 aacctgctgc gccgctgctg ccactacatt gtgaccatgt aactagaggg ccctattcta
     7861 tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     7921 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7981 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8041 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     8101 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     8161 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8221 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8281 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8341 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8401 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8461 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8521 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8581 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     8641 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     8701 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8761 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8821 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8881 ctgcctctga gctattccag aagtagtgag g
//
