LOCUS       sequence-415598-       16161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) 
            resistance marker and sgRNA targeting albA locus in A. niger.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16161)
  AUTHORS   Vanegas KG, Rendsvig JKH, Jarczynska ZD, Cortes MVCB, van Esch AP, 
            Morera-Gomez M, Contesini FJ, Mortensen UH
  TITLE     A Mad7 System for Genetic Engineering of Filamentous Fungi.
  JOURNAL   J Fungi (Basel). 2022 Dec 22;9(1):16. doi: 10.3390/jof9010016.
  PUBMED    36675838
REFERENCE   2  (bases 1 to 16161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2022-12-22"; volume: "9(1):16. doi"; pages: " 
            10.3390/jof9010016"
COMMENT     Sequence Label: pDIV313
FEATURES             Location/Qualifiers
     source          1..16161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      1590..6838
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             12712..12732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13564..14589
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(14661..14694)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(15164..15180)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15279..15296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15450..16038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15530..15549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16160..16161,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt tggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa atatgggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
     1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
     1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
     1441 tagtcaccaa ctagtctatc aagactcaca gggtacattg ctgagaccaa ctgaccagag
     1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
     1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
     1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
     1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
     1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
     1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
     1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
     1921 aaacaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat
     1981 aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag
     2041 tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct
     2101 gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga
     2161 ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt
     2221 attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc
     2281 tcattttcat gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac
     2341 cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt
     2401 ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat
     2461 tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca
     2521 ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc
     2581 tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct
     2641 gagactagtt agaatctagt cccaaactag cttkgatagc ttacctttgc cctttgcgtt
     2701 gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc
     2761 ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta
     2821 cggggctgaa tgtaacttgc cyttcctttc tcatcgcgcg gcaagacagg cttgctcaaa
     2881 ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag
     2941 ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat
     3001 ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat
     3061 gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct
     3121 gctatatatt agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct
     3181 tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta
     3241 cttgactagt cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat
     3301 cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt
     3361 gtccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa
     3421 taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact
     3481 cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc
     3541 agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc
     3601 tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta
     3661 tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga
     3721 ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct
     3781 atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttca
     3841 actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg
     3901 gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg
     3961 ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc
     4021 gaccaccgag gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac
     4081 aaccccacct tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca
     4141 gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg
     4201 ttaactaggt caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc
     4261 caaacataca cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga
     4321 gcccagccat ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc
     4381 aatggaaacg gtgagagtcc agtggtcgca gataactccc taaattccct gagctaactc
     4441 taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt
     4501 acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta
     4561 tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga
     4621 tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc
     4681 actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc
     4741 aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca
     4801 ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg
     4861 acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga
     4921 tatccatcca tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct
     4981 actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga
     5041 aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac
     5101 aatggacctc ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac
     5161 ttggtgacta gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg
     5221 ttctcccaac cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc
     5281 taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt
     5341 gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac
     5401 tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac
     5461 agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc
     5521 cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa
     5581 aggaarggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag
     5641 ccactcttta tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa
     5701 ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atcmaagcta
     5761 gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt
     5821 ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc
     5881 agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc
     5941 ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt
     6001 cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag
     6061 aaagtggtaa gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt
     6121 aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca
     6181 gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc
     6241 aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg
     6301 ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt
     6361 agttgcaact aacctggatc tcagrgactt tggcctcaat ctagaatcta tctagttgtc
     6421 aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag
     6481 tctccctaat atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc
     6541 cccttctagg ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta
     6601 gtttctaact agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa
     6661 cgtttatacg tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta
     6721 gtttataatt agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt
     6781 aacaagatac cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagctta
     6841 acaatctatt ctccgcttgg tgatgctaaa gggctttcaa tagaccttgt aagtgaagga
     6901 gatggagccg tcaatccgct accctgcctc tggtcagttg gtctcagcaa tgtaccctgt
     6961 gagtcttgat agactagttg gtgactagtc tctgtagatg gaacaaatgg tgcttgagag
     7021 ggcacgtatg gcagagctcc agtcatctgc ggaacatata ctgggcccgg gaagatctca
     7081 tggtcatagc tgtttccgct gagggtttaa tgctgagggt ttaatgatca catagatgct
     7141 cggttgacag gaccacctat agcaagtact ttgtaggagg ttctcctaac gcttggcttc
     7201 aaatccaatc aggatatcag tgagttactg tttgtcatcg catcctcatg aagtcgctca
     7261 acagccactg agaagcaaat atttgggaga ccatccctga tgttgaaatt ttacgctgga
     7321 gcccattcac cggtgagctc ggggacagtc tggccagtgg agcggaaaat cttcaactaa
     7381 ctctgattgg taccacaggt agccccacag aacaaaccga gcaaacatga aaattttcgc
     7441 ttgaggttag cgcactcgct agcgcctgcc cgcaaatata aaaggccccg aaatcccatg
     7501 cattttggaa atcaagcgac tctacgtatg tcatccccct ccctaccttt gggaaaacct
     7561 ctcaactgca agtcagaaca ttttgctaac agcgcatcat tggtctagtg gtagaattca
     7621 tcgttgccat cgatgaggcc cgtgttcgat tcacggatga tgcagtcaaa agaccttttt
     7681 aatttctact cttgtagatc agcaatgctt ccatgcaatt gcatcattgg tctagtggta
     7741 gaattcatcg ttgccatcga tgaggcccgt gttcgattca cggatgatgc atgtcaattt
     7801 ttacacttga tgttggtgta atcaggcgat cagagtttgc tcaaattctc tcttgtttta
     7861 gattctgaag aatataattt ttacatgact agttcatttt acttccaagt agactatttg
     7921 tagattgaga ttgccgtcaa ctttattcat agaatcacat taaattgctc attgacctgc
     7981 atgtctatgc acacattcac atctatcttc tcagggtatt aagacctcag ccgagacagc
     8041 agaatcaccg cccaagttaa gcctttgtgc tgatcatgct ctcgaacggg ccaagttcgg
     8101 gaaaagcaaa ggagcgttta gtgaggggca atttgactca cctcccaggc aacagatgag
     8161 gggggcaaaa agaaagaaat tttcgtgagt caatatggat tccgagcatc attttcttgc
     8221 ggtctatctt gctacgtatg ttgatcttga cgctgtggat caagcaacgc cactcgctcg
     8281 ctccatcgca ggctggtcgc agacaaatta aaaggcggca aactcgtaca gccgcggggt
     8341 tgtccgctgc aaagtacaga gtgataaaag ccgccatgcg accatcaacg cgttgatgcc
     8401 cagctttttc gatccgagaa tccaccgtag aggcgatagc aagtaaagaa aagctaaaca
     8461 aaaaaaaatt tctgccccta agccatgaaa acgagatggg gtggagcaga accaaggaaa
     8521 gagtcgcgct gggctgccgt tccggaaggt gttgtaaagg ctcgacgccc aaggtgggag
     8581 tctaggagaa gaatttgcat cgggagtggg gcgggttacc cctccatatc caatgacaga
     8641 tatctaccag ccaagggttt gagcccgccc gcttagtcgt cgtcctcgct tgcccctcca
     8701 taaaaggatt tcccctcccc ctcccacaaa attttctttc ccttcctctc cttgtccgct
     8761 tcagtacgta tatcttccct tccctcgctt ctctcctcca tccttctttc atccatctcc
     8821 tgctaacttc tctgctcagc acctctacgc attactagcc gtagtatctg agcacttctc
     8881 ccttttatat tccacaaaac ataacacaac cttcaccata tgaacaacgg caccaacaac
     8941 ttccagaact tcatcggcat ctccagcctc caaaagaccc tccgcaacgc tctcatcccc
     9001 accgagacta cccagcagtt catcgtcaag aacggcatca tcaaagagga tgagctgcgc
     9061 ggcgagaacc gccagatcct caaggatatc atggatgatt actaccgcgg ctttatctcc
     9121 gagactctct cctccatcga tgatatcgat tggaccagcc tcttcgagaa gatggaaatc
     9181 cagctgaaga acggcgataa caaggatacc ctgatcaaag agcagaccga gtaccgcaag
     9241 gccatccaca agaagttcgc caacgacgat cgcttcaaga atatgttctc cgccaagctc
     9301 atctccgata tcctgcctga gttcgtcatc cacaacaaca actactccgc ctccgagaaa
     9361 gaggaaaaga cccaggtcat caagctgttc tcccgcttcg ccacctcctt caaggattac
     9421 ttcaagaacc gcgccaactg cttctccgcc gacgatatct cctccagctc ctgccaccgc
     9481 atcgtcaacg ataacgccga gatcttcttc tccaacgctc tggtctaccg ccgcatcgtg
     9541 aagtccctct ccaacgatga catcaacaag atctccggcg atatgaagga ttccctcaaa
     9601 gagatgtccc tcgaggaaat ctactcctac gagaagtacg gcgagttcat tacccaagag
     9661 ggcatctcct tctacaacga tatctgcggc aaggtcaact ccttcatgaa cctctactgc
     9721 cagaagaaca aagagaacaa gaacctgtac aagctccaga agctccacaa gcagatcctc
     9781 tgtatcgccg ataccagcta cgaggtcccc tacaagttcg agtccgatga ggaagtctac
     9841 cagtccgtca acggcttcct cgataacatc agctccaagc acatcgtcga gcgcctccgc
     9901 aagatcggcg acaactacaa cggctacaac ctcgataaga tctacatcgt gtccaagttc
     9961 tacgagtctg tctcgcaaaa gacctaccgc gattgggaaa ccatcaacac cgctctcgag
    10021 atccattaca acaacatctt gcccggcaac ggcaagtcca aggccgataa ggtcaagaag
    10081 gccgttaaga acgatctgca gaagtccatc accgagatca acgagctggt gtccaactac
    10141 aagctctgct ccgatgacaa catcaaggcc gaaacctaca tccacgagat ctcccacatc
    10201 ctgaacaact tcgaggccca agagctgaag tacaaccccg agatccacct cgtcgagagc
    10261 gagctgaagg cctccgagtt gaagaacgtc ctcgatgtca tcatgaacgc cttccactgg
    10321 tgctccgtgt tcatgaccga ggaactcgtc gataaggata acaacttcta cgccgagctg
    10381 gaagaaatct acgatgagat ctaccccgtc atctccctgt acaacctggt ccgcaactac
    10441 gtcacccaga agccttactc caccaagaag atcaagctca acttcggcat ccccactctc
    10501 gccgatggtt ggtccaagtc caaagagtac tccaacaacg ccatcatcct catgcgcgat
    10561 aacctgtact acctcggcat cttcaacgcc aagaacaagc ccgataagaa gattatcgag
    10621 ggcaacacca gcgagaacaa gggcgattac aagaagatga tctacaacct gctgcctggt
    10681 cctaacaaga tgatccccaa ggtgttcctg tcctccaaga ccggcgtcga gacttacaag
    10741 ccctccgctt acatcctcga aggctacaag cagaacaagc acattaagtc cagcaaggat
    10801 ttcgatatca ccttctgcca cgatctgatc gactatttca agaactgcat tgccatccat
    10861 cctgagtgga agaacttcgg cttcgatttc tccgatacct ccacctacga ggatatctcc
    10921 ggcttctacc gcgaggtcga gctgcagggc tacaagatcg actggaccta catcagcgag
    10981 aaggatatcg atctcctgca agagaagggc cagctctacc tcttccagat ctataacaaa
    11041 gatttctcca agaagtccac cggcaacgac aacctccaca ccatgtacct caagaacctc
    11101 ttcagcgagg aaaacctcaa ggacatcgtc ctcaagctga acggcgaggc tgaaatcttc
    11161 ttcagaaagt cctccatcaa gaaccccatc atccataaga agggctccat cctcgtcaac
    11221 cgcacctacg aagccgagga aaaggatcag ttcggcaaca tccagatcgt ccgcaagaac
    11281 atccccgaga acatctacca agagttgtac aagtacttca acgacaagtc cgacaaagag
    11341 ctgtccgacg aggccgcaaa gctcaagaac gtcgtcggcc accatgaggc cgccaccaac
    11401 atcgttaagg attaccgcta tacctacgac aagtatttcc tgcacatgcc catcaccatc
    11461 aacttcaagg ccaacaagac cggtttcatc aacgatcgca tcctccagta tatcgccaaa
    11521 gagaaggatc tccacgtcat cggtatcgat cgcggcgagc gcaacctcat ctacgtgtcc
    11581 gtcatcgata cctgcggtaa catcgtggaa cagaagtcct tcaacatcgt caatggctac
    11641 gattaccaga tcaagttgaa gcagcaagag ggcgctcgcc agatcgctcg caaagagtgg
    11701 aaagagatcg gcaagatcaa agaaatcaaa gagggctacc tgagcctggt gatccatgag
    11761 atttccaaga tggtgatcaa gtacaacgct atcattgcca tggaagatct gtcctacggt
    11821 ttcaagaagg gccgcttcaa ggtcgaacgc caggtctacc aaaagttcga aaccatgctg
    11881 atcaacaagt tgaactacct cgtctttaag gacatctcca ttaccgagaa cggcggcctc
    11941 ctcaagggct accagctcac ctacattccc gataagctga aaaacgtcgg ccatcagtgc
    12001 ggctgcatct tctacgtccc tgccgcctac acctccaaga tcgatcccac caccggcttc
    12061 gtcaacatct tcaagttcaa ggatctcacc gtcgatgcca agcgcgaatt catcaagaag
    12121 tttgattcca tccgctacga ttccgagaag aatctcttct gtttcacctt cgactacaac
    12181 aatttcatca ctcagaacac cgtcatgtcc aagtcgtcct ggtccgtcta cacctacggc
    12241 gtccgcatca agcgccgctt cgttaacggc cgcttctcga acgagtccga caccatcgac
    12301 atcaccaagg atatggaaaa gacactcgag atgaccgata tcaactggcg cgacggccac
    12361 gacctccgcc aggacatcat cgactacgag atcgtccagc atatcttcga aatcttccgc
    12421 ctcaccgtcc agatgcgcaa ctccctgtcc gagttggagg atcgcgacta cgatcgcctc
    12481 atctcccctg tcctcaacga gaacaacatt ttctacgata gcgccaaggc cggcgacgcc
    12541 ctgcctaagg atgccgacgc caacggcgcc tactgtatcg ctctcaaggg cctgtacgag
    12601 atcaagcaaa tcaccgaaaa ctggaaagag gacggaaagt tcagccgcga caagctgaag
    12661 atctccaaca aggattggtt cgatttcatc caaaacaagc gctacctgcc tcccaagaag
    12721 aagcgcaagg tctgaagcgg acattcgatt tatgccgtta tgacttcctt aaaaaagcct
    12781 ttacgaatga aagaaatgga attagacttg ttatgtagtt gattctacaa tggattatga
    12841 ttcctgaact tcaaatccgc tgttcattat taatctcagc tcttcccgta aagccaatgt
    12901 tgaaactatt cgtaaatgta cctcgttttg cgtgtacctt gcttatcacg tgatattaca
    12961 tgacctggac agagttctgc gcgaaagtca taacgtaaat cccgggcggt aggtgcgtcc
    13021 cgggcggaag gtagttttct cgtccacccc aacgcgttta tcaacctcaa ctttcaacaa
    13081 ccatcatgcc accaaaagcg cgtaaaacaa agcgagattt gattgagcaa gagggcagga
    13141 tccaatgcgc gattcaagac attaaaaatg gaaaatttca aaaaattgcg cccgcagcgc
    13201 gtgcatacaa aattcatccc aatacattaa gacctcagcg ctagtggagg tcaacacatc
    13261 aatgcctatt ttggtttagt cgtccaggcg gtgagcacaa aatttgtgtc gtttgacaag
    13321 atggttcatt taggcaactg gtcagatcag ccccacttgt agcagtagcg gcggcgctcg
    13381 aagtgtgact cttattagca gacaggaacg aggacattat tatcatctgc tgcttggtgc
    13441 acgataactt ggtgcgtttg tcaagcaagg taagtggacg acccggtcat accttcttaa
    13501 gttcgccctt cctcccttta tttcagattc aatctgactt acctattcta cccaagcatc
    13561 caaatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
    13621 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
    13681 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
    13741 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
    13801 attggggagt tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
    13861 ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc agccggtcgc ggaggccatg
    13921 gatgcgattg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
    13981 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
    14041 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
    14101 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca tgcggatttc
    14161 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
    14221 gcgatgttcg gggattccca atacgaggtc gccaacatcc tcttctggag gccgtggttg
    14281 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    14341 ccgcgcctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    14401 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    14461 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    14521 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
    14581 aaggaataga gtagatgccg accgatcccc actaccgcat taagacctca gcgcggccgc
    14641 aaatttaaat aaaatgaagt gaagttccta tactttctag agaataggaa cttctatagt
    14701 gagtcgaata agggcgacac aaaatttatt ctaaatgcat aataaatact gataacatct
    14761 tatagtttgt attatatttt gtattatcgt tgacatgtat aattttgata tcaaaaactg
    14821 attttccctt tattattttc gagatttatt ttcttaattc tctttaacaa actagaaata
    14881 ttgtatatac aaaaaatcat aaataataga tgaatagttt aattataggt gttcatcaat
    14941 cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat aaggttaaat
    15001 aattctcata tatcaagcaa agtgacaggc gcccttaaat attctgacaa atgctctttc
    15061 cctaaactcc ccccataaaa aaacccgccg aagcgggttt ttacgttatt tgcggattaa
    15121 cgattactcg ttatcagaac cgcccagggg gcccgagctt aagactggcc gtcgttttac
    15181 aacacagaaa gagtttgtag aaacgcaaaa aggccatccg tcaggggcct tctgcttagt
    15241 ttgatgcctg gcagttccct actctcgcct tccgcttcct cgctcactga ctcgctgcgc
    15301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    15361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    15421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    15481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    15541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    15601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    15661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    15721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    15781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    15841 ggtgctacag agttcttgaa gtggtgggct aactacggct acactagaag aacagtattt
    15901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    15961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    16021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    16081 aacgacgcgc gcgtaactca cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca
    16141 agtcagcgta atgctctgct t
//
