LOCUS       sequence-415602-       10584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing 
            mouse Oct4 + Klf4 + super-Sox).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17
FEATURES             Location/Qualifiers
     source          1..10584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3756..3812
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5241..5808
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5258..5810
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5402..5419)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5629..5648
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    7026..7614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7079..7099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7497..7508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7762..7942
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7989..8007)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8075..8179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8180..8248,8249..9040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8398..8417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9211..9799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9953..9970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10210..10231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10246..10251,10252..10269,10270..10276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10284..10300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10289..10311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10308..10324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10308..10324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10374..10393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
     2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
     2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
     2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
     2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
     3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
     3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
     3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
     3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
     3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
     3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
     3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
     3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
     3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
     3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
     3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
     3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
     3721 gctctcccat gcattcaaac acgcgtggat ctggagccac caactttagt ctcctcaaac
     3781 aagccggtga cgttgaagaa aatccaggcc ccatggctgt cagcgacgct ctgctcccgt
     3841 ccttctccac gttcgcgtcc ggcccggcgg gaagggagaa gacactgcgt ccagcaggtg
     3901 ccccgactaa ccgttggcgt gaggaactct ctcacatgaa gcgacttccc ccacttcccg
     3961 gccgccccta cgacctggcg gcgacggtgg ccacagacct ggagagtggc ggagctggtg
     4021 cagcttgcag cagtaacaac ccggccctcc tagcccggag ggagaccgag gagttcaacg
     4081 acctcctgga cctagacttt atcctttcca actcgctaac ccaccaggaa tcggtggccg
     4141 ccaccgtgac cacctcggcg tcagcttcat cctcgtcttc cccagcgagc agcggccctg
     4201 ccagcgcgcc ctccacctgc agcttcagct atccgatccg ggccgggggt gacccgggcg
     4261 tggctgccag caacacaggt ggagggctcc tctacagccg agaatctgcg ccacctccca
     4321 cggccccctt caacctggcg gacatcaatg acgtgagccc ctcgggcggc ttcgtggctg
     4381 agctcctgcg gccggagttg gacccagtat acattccgcc acagcagcct cagccgccag
     4441 gtggcgggct gatgggcaag tttgtgctga aggcgtctct gaccacccct ggcagcgagt
     4501 acagcagccc ttcggtcatc agtgttagca aaggaagccc agacggcagc caccccgtgg
     4561 tagtggcgcc ctacagcggt ggcccgccgc gcatgtgccc caagattaag caagaggcgg
     4621 tcccgtcctg cacggtcagc cggtccctag aggcccattt gagcgctgga ccccagctca
     4681 gcaacggcca ccggcccaac acacacgact tccccctggg gcggcagctc cccaccagga
     4741 ctacccctac actgagtccc gaggaactgc tgaacagcag ggactgtcac cctggcctgc
     4801 ctcttccccc aggattccat ccccatccgg ggcccaacta ccctcctttc ctgccagacc
     4861 agatgcagtc acaagtcccc tctctccatt atcaagagct catgccaccg ggttcctgcc
     4921 tgccagagga gcccaagcca aagaggggaa gaaggtcgtg gccccggaaa agaacagcca
     4981 cccacacttg tgactatgca ggctgtggca aaacctatac caagagttct catctcaagg
     5041 cacacctgcg aactcacaca ggcgagaaac cttaccactg tgactgggac ggctgtgggt
     5101 ggaaattcgc ccgctcggat gaactgacca ggcactaccg caaacacaca gggcaccggc
     5161 cctttcagtg ccagaagtgc gacagggcct tttccaggtc ggaccacctt gccttacaca
     5221 tgaagaggca cttttaaaga tccctccccc ccccctaacg ttactggccg aagccgcttg
     5281 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5341 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5401 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5461 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5521 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5581 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5641 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5701 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     5761 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca
     5821 tatgatgtat aacatgatgg agacggagct gaagccgccg ggcccgcagc aagcttcggg
     5881 gggcggcggc ggaggaggca acgccacggc ggcggcaacc ggcggcaacc agaagaacag
     5941 cccggaccgc gtcaagaggc ccatgaacgc cttcatggta tggtcccggg ggcagcggcg
     6001 taagatggcc caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc
     6061 ggagtggaaa gcgttgacct tggcagagaa gcggccgttc atcgacgagg ccaagcggct
     6121 gcgcgtgctg catatgcagg accaccccaa ctacaagtac cggccgcggc ggcgcaagca
     6181 ggtgaagcgc atgaagcggg tggagggagg cttcctgcac gctctcgtcg agccccaggc
     6241 cggcgcgctt ggtcccgagg gcggccgcgt ggccatggat ggcctgggtc tgcctttccc
     6301 ggagccgggc tatccggccg gtcctccgct gatgtctccg cacatgggcc cccactatcg
     6361 ggactgccag ggactgggcg ctcccgcgct cgacggctac cctctgccca ctccggacac
     6421 atccccgctg gatggcgtgg agcaggaccc ggctttcttt gcagccccgc tgccagggga
     6481 ctgcccggcg gccggcacct acacttacgc tccagtctcg gactatgcag tgtccgtaga
     6541 gccgcccgct ggccccatgc gagtggggcc ggacccctcg ggccctgcga tgccggggat
     6601 cctggcgccc cccagcgctc tgcacctgta ctacggcgcg atgggctcgc ccgccgcaag
     6661 tgcggggcgc ggtttccacg cgcaacccca gcagccgctg caaccgcagg caccgccccc
     6721 gccaccgcag cagcagcacc cagcgcacgg ccccgggcaa ccttcgcccc ctcccgaggc
     6781 tctgccctgc cgggatggca cggaatccaa ccagcccact gagctcctag gggaggtgga
     6841 ccgcacggaa tttgaacagt atctgccctt tgtgtataag cccgagatgg gtcttcccta
     6901 ccagggacac gactgcggag tgaacctctc agacagccac ggagccattt cctccgtggt
     6961 gtccgacgct agctcagcgg tctactattg caactacccc gacatttgat aaatcgatag
     7021 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7081 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7141 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7201 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7261 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7321 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7381 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7441 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7501 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7561 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     7621 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7681 gggggactgg aagggctaat tcactcccaa cgaagacaag atcacctgca ggacaggcgc
     7741 gccctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7801 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7861 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7921 tcagtgtgga aaatctctag cacccgggcg attaaggaaa gggctagatc attcttgaag
     7981 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8041 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8101 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8161 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8221 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8281 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8341 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8401 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     8461 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8521 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8581 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8641 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8701 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8761 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8821 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8881 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8941 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9001 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9061 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9121 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9601 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9841 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9901 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9961 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    10021 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag
    10081 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    10141 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    10201 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    10261 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    10321 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10381 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10441 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    10501 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    10561 aatatgtact acaaacttag tagt
//
