LOCUS       sequence-415603-       15462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15462)
  AUTHORS   Vanegas KG, Rendsvig JKH, Jarczynska ZD, Cortes MVCB, van Esch AP, 
            Morera-Gomez M, Contesini FJ, Mortensen UH
  TITLE     A Mad7 System for Genetic Engineering of Filamentous Fungi.
  JOURNAL   J Fungi (Basel). 2022 Dec 22;9(1):16. doi: 10.3390/jof9010016.
  PUBMED    36675838
REFERENCE   2  (bases 1 to 15462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2022-12-22"; volume: "9(1):16. doi"; pages: " 
            10.3390/jof9010016"
COMMENT     Sequence Label: pDIV298
FEATURES             Location/Qualifiers
     source          1..15462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      1590..6840
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             11820..11840
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(13962..13995)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(14465..14481)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14580..14597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14751..15339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14831..14850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15461..15462,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
     1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
     1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
     1441 tagtcaccaa ctagtctatc aagactcaca gggtacattg ctgagaccaa ctgaccagag
     1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
     1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
     1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
     1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
     1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
     1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
     1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
     1921 aaaacaagac agccacatat tagggagact agttagaagc tagttccagg actaggaaaa
     1981 taaaagacaa tgataccaca gtctagttga caactagata gattctagat tgaggccaaa
     2041 gtctctgaga tccaggttag ttgcaactaa tactagttag tatctagtct cctataactc
     2101 tgaagctaga ataacttact actattatcc tcaccactgt tcagctgcgc aaacggagtg
     2161 attgcaaggt gttcagagac tagttattga ctagtcagtg actagcaata actaacaagg
     2221 tattaaccta ccatgtctgc catcaccctg cacttcctcg ggctcagcag ccttttcctc
     2281 ctcattttca tgctcatttt ccttgtttaa gactgtgact agtcaaagac tagtccagaa
     2341 ccacaaagga gaaatgtctt accactttct tcattgcttg tctcttttgc attatccatg
     2401 tctgcaacta gttagagtct agttagtgac tagtccgacg aggacttgct tgtctccgga
     2461 ttgttggagg aactctccag ggcctcaaga tccacaacag agccttctag aagactggtc
     2521 aataactagt tggtctttgt ctgagtctga cttacgaggt tgcatactcg ctccctttgc
     2581 ctcgtcaatc gatgagaaaa agcgccaaaa ctcgcaatat ggctttgaac cacacggtgc
     2641 tgagactagt tagaatctag tcccaaacta gcttggatag cttacctttg ccctttgcgt
     2701 tgcgacaggt cttgcagggt atggttcctt tctcaccagc tgatttagct gccttgctac
     2761 mctcacggcg gatctgcata aagagtggct agaggttata aattagcact gatcctaggt
     2821 acggggctga atgtaacttg ccyttccttt ctcatcgcgc ggcaagacag gcttgctcaa
     2881 attcctacca gtcacagggg tatgcacggc gtacggacca cttgaactag tcacagatta
     2941 gttagcaact agtctgcatt gaatggctgt acttacgggc cctcgccatt gtcctgatca
     3001 tttccagctt caccctcgtt gctgcaaagt agttagtgac tagtcaagga ctagttgaaa
     3061 tgggagaaga aactcacgaa ttctcgacac ccttagtatt gtggtccttg gacttggtgc
     3121 tgctatatat tagctaatac actagttaga ctcacagaaa cttacgcagc tcgcttgcgc
     3181 ttcttggtag gagtcggggt tgggagaaca gtgccttcaa acaagccttc ataccatgct
     3241 acttgactag tcagggacta gtcaccaagt aatctagata ggacttkcct ttggcctcca
     3301 tcagttcctt catagtggga ggtccattgt gcaatgtaaa ctccatgccg tgggagttct
     3361 tgtccttcaa gtgcttgacc aatatgtttc tgttggcaga gggaacctgt caactagtta
     3421 ataactagtc agaaactagt atagcagtag actcactgta cgcttgaggc atcccttcac
     3481 tcggcagtag acttcatatt gatggatatc aggcacgcca ttgtcgtcct gtggactagt
     3541 cagtaactag gcttaaagct agtcgggtcg gcttactatc ttgaaatccg gcagcgtaag
     3601 ctccccgtcc ttaactgcct cgagatagtg acagtactct ggggactttc ggagatcgtt
     3661 atcgcgaatg ctcggcatac taatcgttga ctagtcttgg actagtcccg agcaaaaagg
     3721 attggaggag gaggaggaag gtgagagtga gacaaagagc gaaataagag cttcaaaggc
     3781 tatctctaag cagtatgaag gttaagtatc tagttcttga ctagatttaa aagagatttc
     3841 gactagttat gtacctggag tttggatata ggaatgtgtt gtggtaacga aatgtaaggg
     3901 ggaggaaaga aaaagtcggt caagaggtaa ctctaagtcg gccattcctt tttgggaggc
     3961 gctaaccata aacggcatgg tcgacttaga gttagctcag ggaatttagg gagttatctg
     4021 cgaccaccga ggaacggctg aatgccaaag aatcccgatg gagctctagc tggcggttga
     4081 caaccccacc ttttggcgtt tctgcggcgt tgcaggcggg actggatact tcgtagaacc
     4141 agaaaggcaa ggcagaacgc gctcagcaag agtgttggaa gtgatagcat gatgtgcctt
     4201 gttaactagg tcaaaatctg cagtatgctt gatgttatcc aaagtgtgag agaggaaggt
     4261 ccaaacatac acgattggga gagggcctag gtataagagt ttttgagtag aacgcatgtg
     4321 agcccagcca tctcgaggag attaaacacg ggccggcatt tgatggctat gttagtaccc
     4381 caatggaaac ggtgagagtc cagtggtcgc agataactcc ctaaattccc tgagctaact
     4441 ctaagtcgac catgccgttt atggttagcg cctcccaaaa aggaatggcc gacttagagt
     4501 tacctcttga ccgacttttt ctttcctccc ccttacattt cgttaccaca acacattcct
     4561 atatccaaac tccaggtaca taactagtcg aaatctcttt taaatctagt caagaactag
     4621 atacttaacc ttcatactgc ttagagatag cctttgaagc tcttatttcg ctctttgtct
     4681 cactctcacc ttcctcctcc tcctccaatc ctttttgctc gggactagtc caagactagt
     4741 caacgattag tatgccgagc attcgcgata acgatctccg aaagtcccca gagtactgtc
     4801 actatctcga ggcagttaag gacggggagc ttacgctgcc ggatttcaag atagtaagcc
     4861 gacccgacta gctttaagcc tagttactga ctagtccaca ggacgacaat ggcgtgcctg
     4921 atatccatcc atatgaagtc tactgccgag tgaagggatg cctcaagcgt acagtgagtc
     4981 tactgctata ctagtttctg actagttatt aactagttga caggttccct ctgccaacag
     5041 aaacatattg gtcaagcact tgaaggacaa gaactcccac ggcatggagt ttacattgca
     5101 caatggacct cccactatga aggaactgat ggaggccaaa ggcaagtcct atctagatta
     5161 cttggtgact agtccctgac tagtcaagta gcatggtatg aaggcttgtt tgaaggcact
     5221 gttctcccaa ccccgactcc taccaagaag cgcaagcgag ctgcgtaagt ttctgtgagt
     5281 ctaactagtg tattagctaa tatatagcag caccaagtcc aaggaccaca atactaaggg
     5341 tgtcgagaat tcgtgagttt cttctcccat ttcaactagt ccttgactag tcactaacta
     5401 ctttgcagca acgagggtga agctggaaat gatcaggaca atggcgaggg cccgtaagta
     5461 cagccattca atgcagacta gttgctaact aatctgtgac tagttcaagt ggtccgtacg
     5521 ccgtgcatac ccctgtgact ggtaggaatt tgagcaagcc tgtcttgccg cgcgatgaga
     5581 aaggaarggc aagttacatt cagccccgta cctaggatca gtgctaattt ataacctcta
     5641 gccactcttt atgcagatcc gccgtgagkg tagcaaggca gctaaatcag ctggtgagaa
     5701 aggaaccata ccctgcaaga cctgtcgcaa cgcaaagggc aaaggtaagc tatccaagct
     5761 agtttgggac tagattctaa ctagtctcag caccgtgtgg ttcaaagcca tattgcgagt
     5821 tttggcgctt tttctcatcg attgacgagg caaagggagc gagtatgcaa cctcgtaagt
     5881 cagactcaga caaagaccaa ctagttattg accagtcttc tagaaggctc tgttgtggat
     5941 cttgaggccc tggagagttc ctccaacaat ccggagacaa gcaagtcctc gtcggactag
     6001 tcactaacta gactctaact agttgcagac atggataatg caaaagagac aagcaatgaa
     6061 gaaagtggta agacatttct cctttgtggt tctggactag tctttgacta gtcacagtct
     6121 taaacaagga aaatgagcat gaaaatgagg aggaaaaggc tgctgagccc gaggaagtgc
     6181 agggtgatgg cagacatggt aggttaatac cttgttagtt attgctagtc actgactagt
     6241 caataactag tctctgaaca ccttgcaatc actccgtttg cgcagctgaa cagtggtgag
     6301 gataatagta gtaagttatt ctagcttcag agttatagga gactagatac taactagtat
     6361 tagttgcaac taacctggat ctcagagact ttggcctcaa tctagaatct atctagttgt
     6421 caactagact gtggtatcat tgtcttttat tttcctagtc ctggaactag cttctaacta
     6481 gtctccctaa tatgtggctg tcttgttttt tttttttngt ttccctaccc ggatatctag
     6541 tccccttcta ggttctgtta acctctcggg ctctgattta gtttaacgca aacctgagat
     6601 tagtttctaa ctagtctcta ggttttctat ccacctttaa ttgtaataat aaatacaagc
     6661 aacgtttata cgtcaaaagc atttataaac ttttacccta aagtagcttg cttgtgtgtt
     6721 tagtttataa ttagtctctt attaatttga tgtaggtaag cccgccacaa atatatattt
     6781 ttaacaagat accgtggaaa aacttcgtgc tatcacaaaa cagtatacaa aaaataagct
     6841 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
     6901 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
     6961 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
     7021 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
     7081 catggtcata gctgtttccg ctgagggttt aatgctgagg gtttaattaa gacctcagcc
     7141 gagacagcag aatcaccgcc caagttaagc ctttgtgctg atcatgctct cgaacgggcc
     7201 aagttcggga aaagcaaagg agcgtttagt gaggggcaat ttgactcacc tcccaggcaa
     7261 cagatgaggg gggcaaaaag aaagaaattt tcgtgagtca atatggattc cgagcatcat
     7321 tttcttgcgg tctatcttgc tacgtatgtt gatcttgacg ctgtggatca agcaacgcca
     7381 ctcgctcgct ccatcgcagg ctggtcgcag acaaattaaa aggcggcaaa ctcgtacagc
     7441 cgcggggttg tccgctgcaa agtacagagt gataaaagcc gccatgcgac catcaacgcg
     7501 ttgatgccca gctttttcga tccgagaatc caccgtagag gcgatagcaa gtaaagaaaa
     7561 gctaaacaaa aaaaaatttc tgcccctaag ccatgaaaac gagatggggt ggagcagaac
     7621 caaggaaaga gtcgcgctgg gctgccgttc cggaaggtgt tgtaaaggct cgacgcccaa
     7681 ggtgggagtc taggagaaga atttgcatcg ggagtggggc gggttacccc tccatatcca
     7741 atgacagata tctaccagcc aagggtttga gcccgcccgc ttagtcgtcg tcctcgcttg
     7801 cccctccata aaaggatttc ccctccccct cccacaaaat tttctttccc ttcctctcct
     7861 tgtccgcttc agtacgtata tcttcccttc cctcgcttct ctcctccatc cttctttcat
     7921 ccatctcctg ctaacttctc tgctcagcac ctctacgcat tactagccgt agtatctgag
     7981 cacttctccc ttttatattc cacaaaacat aacacaacct tcaccatatg aacaacggca
     8041 ccaacaactt ccagaacttc atcggcatct ccagcctcca aaagaccctc cgcaacgctc
     8101 tcatccccac cgagactacc cagcagttca tcgtcaagaa cggcatcatc aaagaggatg
     8161 agctgcgcgg cgagaaccgc cagatcctca aggatatcat ggatgattac taccgcggct
     8221 ttatctccga gactctctcc tccatcgatg atatcgattg gaccagcctc ttcgagaaga
     8281 tggaaatcca gctgaagaac ggcgataaca aggataccct gatcaaagag cagaccgagt
     8341 accgcaaggc catccacaag aagttcgcca acgacgatcg cttcaagaat atgttctccg
     8401 ccaagctcat ctccgatatc ctgcctgagt tcgtcatcca caacaacaac tactccgcct
     8461 ccgagaaaga ggaaaagacc caggtcatca agctgttctc ccgcttcgcc acctccttca
     8521 aggattactt caagaaccgc gccaactgct tctccgccga cgatatctcc tccagctcct
     8581 gccaccgcat cgtcaacgat aacgccgaga tcttcttctc caacgctctg gtctaccgcc
     8641 gcatcgtgaa gtccctctcc aacgatgaca tcaacaagat ctccggcgat atgaaggatt
     8701 ccctcaaaga gatgtccctc gaggaaatct actcctacga gaagtacggc gagttcatta
     8761 cccaagaggg catctccttc tacaacgata tctgcggcaa ggtcaactcc ttcatgaacc
     8821 tctactgcca gaagaacaaa gagaacaaga acctgtacaa gctccagaag ctccacaagc
     8881 agatcctctg tatcgccgat accagctacg aggtccccta caagttcgag tccgatgagg
     8941 aagtctacca gtccgtcaac ggcttcctcg ataacatcag ctccaagcac atcgtcgagc
     9001 gcctccgcaa gatcggcgac aactacaacg gctacaacct cgataagatc tacatcgtgt
     9061 ccaagttcta cgagtctgtc tcgcaaaaga cctaccgcga ttgggaaacc atcaacaccg
     9121 ctctcgagat ccattacaac aacatcttgc ccggcaacgg caagtccaag gccgataagg
     9181 tcaagaaggc cgttaagaac gatctgcaga agtccatcac cgagatcaac gagctggtgt
     9241 ccaactacaa gctctgctcc gatgacaaca tcaaggccga aacctacatc cacgagatct
     9301 cccacatcct gaacaacttc gaggcccaag agctgaagta caaccccgag atccacctcg
     9361 tcgagagcga gctgaaggcc tccgagttga agaacgtcct cgatgtcatc atgaacgcct
     9421 tccactggtg ctccgtgttc atgaccgagg aactcgtcga taaggataac aacttctacg
     9481 ccgagctgga agaaatctac gatgagatct accccgtcat ctccctgtac aacctggtcc
     9541 gcaactacgt cacccagaag ccttactcca ccaagaagat caagctcaac ttcggcatcc
     9601 ccactctcgc cgatggttgg tccaagtcca aagagtactc caacaacgcc atcatcctca
     9661 tgcgcgataa cctgtactac ctcggcatct tcaacgccaa gaacaagccc gataagaaga
     9721 ttatcgaggg caacaccagc gagaacaagg gcgattacaa gaagatgatc tacaacctgc
     9781 tgcctggtcc taacaagatg atccccaagg tgttcctgtc ctccaagacc ggcgtcgaga
     9841 cttacaagcc ctccgcttac atcctcgaag gctacaagca gaacaagcac attaagtcca
     9901 gcaaggattt cgatatcacc ttctgccacg atctgatcga ctatttcaag aactgcattg
     9961 ccatccatcc tgagtggaag aacttcggct tcgatttctc cgatacctcc acctacgagg
    10021 atatctccgg cttctaccgc gaggtcgagc tgcagggcta caagatcgac tggacctaca
    10081 tcagcgagaa ggatatcgat ctcctgcaag agaagggcca gctctacctc ttccagatct
    10141 ataacaaaga tttctccaag aagtccaccg gcaacgacaa cctccacacc atgtacctca
    10201 agaacctctt cagcgaggaa aacctcaagg acatcgtcct caagctgaac ggcgaggctg
    10261 aaatcttctt cagaaagtcc tccatcaaga accccatcat ccataagaag ggctccatcc
    10321 tcgtcaaccg cacctacgaa gccgaggaaa aggatcagtt cggcaacatc cagatcgtcc
    10381 gcaagaacat ccccgagaac atctaccaag agttgtacaa gtacttcaac gacaagtccg
    10441 acaaagagct gtccgacgag gccgcaaagc tcaagaacgt cgtcggccac catgaggccg
    10501 ccaccaacat cgttaaggat taccgctata cctacgacaa gtatttcctg cacatgccca
    10561 tcaccatcaa cttcaaggcc aacaagaccg gtttcatcaa cgatcgcatc ctccagtata
    10621 tcgccaaaga gaaggatctc cacgtcatcg gtatcgatcg cggcgagcgc aacctcatct
    10681 acgtgtccgt catcgatacc tgcggtaaca tcgtggaaca gaagtccttc aacatcgtca
    10741 atggctacga ttaccagatc aagttgaagc agcaagaggg cgctcgccag atcgctcgca
    10801 aagagtggaa agagatcggc aagatcaaag aaatcaaaga gggctacctg agcctggtga
    10861 tccatgagat ttccaagatg gtgatcaagt acaacgctat cattgccatg gaagatctgt
    10921 cctacggttt caagaagggc cgcttcaagg tcgaacgcca ggtctaccaa aagttcgaaa
    10981 ccatgctgat caacaagttg aactacctcg tctttaagga catctccatt accgagaacg
    11041 gcggcctcct caagggctac cagctcacct acattcccga taagctgaaa aacgtcggcc
    11101 atcagtgcgg ctgcatcttc tacgtccctg ccgcctacac ctccaagatc gatcccacca
    11161 ccggcttcgt caacatcttc aagttcaagg atctcaccgt cgatgccaag cgcgaattca
    11221 tcaagaagtt tgattccatc cgctacgatt ccgagaagaa tctcttctgt ttcaccttcg
    11281 actacaacaa tttcatcact cagaacaccg tcatgtccaa gtcgtcctgg tccgtctaca
    11341 cctacggcgt ccgcatcaag cgccgcttcg ttaacggccg cttctcgaac gagtccgaca
    11401 ccatcgacat caccaaggat atggaaaaga cactcgagat gaccgatatc aactggcgcg
    11461 acggccacga cctccgccag gacatcatcg actacgagat cgtccagcat atcttcgaaa
    11521 tcttccgcct caccgtccag atgcgcaact ccctgtccga gttggaggat cgcgactacg
    11581 atcgcctcat ctcccctgtc ctcaacgaga acaacatttt ctacgatagc gccaaggccg
    11641 gcgacgccct gcctaaggat gccgacgcca acggcgccta ctgtatcgct ctcaagggcc
    11701 tgtacgagat caagcaaatc accgaaaact ggaaagagga cggaaagttc agccgcgaca
    11761 agctgaagat ctccaacaag gattggttcg atttcatcca aaacaagcgc tacctgcctc
    11821 ccaagaagaa gcgcaaggtc tgaagcggac attcgattta tgccgttatg acttccttaa
    11881 aaaagccttt acgaatgaaa gaaatggaat tagacttgtt atgtagttga ttctacaatg
    11941 gattatgatt cctgaacttc aaatccgctg ttcattatta atctcagctc ttcccgtaaa
    12001 gccaatgttg aaactattcg taaatgtacc tcgttttgcg tgtaccttgc ttatcacgtg
    12061 atattacatg acctggacag agttctgcgc gaaagtcata acgtaaatcc cgggcggtag
    12121 gtgcgtcccg ggcggaaggt agttttctcg tccaccccaa cgcgtttatc aacctcaact
    12181 ttcaacaacc atcatgccac caaaagcgcg taaaacaaag cgagatttga ttgagcaaga
    12241 gggcaggatc caatgcgcga ttcaagacat taaaaatgga aaatttcaaa aaattgcgcc
    12301 cgcagcgcgt gcatacaaaa ttcatcccaa tacattaaga cctcagctcc tcgtgtactg
    12361 tgtaagcgcc cactaggtaa tatgacatga ttacgaattc gagctcggta cccggccggg
    12421 gatcctctag acgtggagtt accagtgatt gaccaatgtt ttatcttcta cagttctgcc
    12481 tgtctacccc attctagctg tacctgacta cagagtagtt taattgtggt tgaccccaca
    12541 gtcggaggcg gaggaataca gcaccgatgt ggcctgtctc catccagatt ggcacgcaat
    12601 ttttacacgc ggaaaagatc gagatagagt acgactttaa atttagtccc cggcggcttc
    12661 tattttagaa tatttgagat ttgattctca agcaattgat ttggttgggt caccctcaat
    12721 tggataatat acctcattgc tcggctactt caactcatca atcaccgtca taccccgcat
    12781 ataaccctcc attcccacga tgtcgtccaa gtcgcaattg acttacggtg ctcgagccag
    12841 caagcacccc aatcctctgg caaagagact ttttgagatt gccgaagcaa agaagacaaa
    12901 cgttaccgtc tctgctgatg tgacgacaac ccgagaactc ctggacctcg ctgaccgtac
    12961 ggaagctgtt ggatccaata catatgccgt ctagcaatgg actaatcaac ttttgatgat
    13021 acaggtctcg gtccctacat cgccgtcatc aagacacaca tcgacatcct caccgatttc
    13081 agcgtcgaca ctatcaatgg cctgaatgtg ctggctcaaa agtacaactt tttgatcttc
    13141 gaggaccgca aattcatcga catcggcaat accgtccaga agcaatacca cggcggtgct
    13201 ctgaggatct ccgaatgggc ccacattatc aactgcagcg ttctccctgg cgagggcatc
    13261 gtcgaggctc tggcccagac cgcatctgcg caagacttcc cctatggtcc tgagagagga
    13321 ctgttggtcc tggcagagat gacccccaaa ggatcgctgg ctacgggcga gtataccaag
    13381 gcatcggttg actacgctcg caaatacaag aacttcgtta tgggtttcgt gtcgacgcgg
    13441 gccctgacgg aagtgcagtc ggatgtgtct tcagcctcgg aggatgaaga tttcgtggtc
    13501 ttcacgacgg gtgtgaacct ctcttccaaa ggagataagc ttggacagca ataccagact
    13561 cctgcatcgg ctattggacg cggtgccgac tttatcatcg ccggtcgagg catctacgct
    13621 gctcccgacc cggttgaagc tgcacagcgg taccagaaag aaggctggga agcttatatg
    13681 gccagagtat gcggcaagtc atgatttcct cttggagcaa aagtgtagtg ccagtacgag
    13741 tgttgtggag gaaggctgca tacattgtgc ctgtcattaa acgatgagct cgtccgtatt
    13801 ggcccctgta atgccatgtt ttccgccccc aatcgtcaag gttttccctt tgttagattc
    13861 ctaccagtca tctagcaagg cggccgcagc tagcacaatt gaggcatccc cactaccgca
    13921 ttaagacctc agcgcggccg caaatttaaa taaaatgaag tgaagttcct atactttcta
    13981 gagaatagga acttctatag tgagtcgaat aagggcgaca caaaatttat tctaaatgca
    14041 taataaatac tgataacatc ttatagtttg tattatattt tgtattatcg ttgacatgta
    14101 taattttgat atcaaaaact gattttccct ttattatttt cgagatttat tttcttaatt
    14161 ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag atgaatagtt
    14221 taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt
    14281 ttctcattta taaggttaaa taattctcat atatcaagca aagtgacagg cgcccttaaa
    14341 tattctgaca aatgctcttt ccctaaactc cccccataaa aaaacccgcc gaagcgggtt
    14401 tttacgttat ttgcggatta acgattactc gttatcagaa ccgcccaggg ggcccgagct
    14461 taagactggc cgtcgtttta caacacagaa agagtttgta gaaacgcaaa aaggccatcc
    14521 gtcaggggcc ttctgcttag tttgatgcct ggcagttccc tactctcgcc ttccgcttcc
    14581 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    14641 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    14701 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    14761 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    14821 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    14881 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    14941 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    15001 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    15061 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    15121 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtgggc taactacggc
    15181 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    15241 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    15301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    15361 acggggtctg acgctcagtg gaacgacgcg cgcgtaactc acgttaaggg attttggtca
    15421 tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc tt
//
