LOCUS       sequence-415629-        5393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-vector for fluorescent calcium measurements in the red spectrum 
            from noradrenergic neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Wissing C, Eschholz LS, Maheu M, Sauter K, Morellini F, Wiegert JS, 
            Dieter A
  TITLE     A comparison of viral strategies and model systems to target 
            norepinephrine neurons in the locus coeruleus reveals high 
            variability in transgene expression patterns.
  JOURNAL   PLoS Biol. 2025 Jul 7;23(7):e3003228. doi: 
            10.1371/journal.pbio.3003228. eCollection 2025 Jul.
  PUBMED    40623068
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2025-07-7"; volume: "23(7):e3003228. doi"; pages: " 
            10.1371/journal.pbio.3003228. eCollection 2025 Jul"
COMMENT     Sequence Label: PRS_8-jRGECO1a
FEATURES             Location/Qualifiers
     source          1..5393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(161..749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(241..260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(920..1711,1712..1780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1543..1562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1781..1885)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1911..2366)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2048..2069)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2260..2279
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2438..2567
                     /label=AAV2 ITR
     CDS             2967..2984
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2988..3020
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(3024..3032,3033..3047)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             3075..3131
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     3827..3846
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4331..4919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4384..4404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4802..4813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4960..5167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
       61 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     1921 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     1981 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     2041 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     2101 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     2161 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     2221 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     2281 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     2341 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     2401 agggcgatcg gtgcgggcct cttcgctatt acgccagctg cgcgctcgct cgctcactga
     2461 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga
     2521 gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt agttaatgat
     2581 taacccgcca tgctacttat ctacgtagcc atgctctagg aagatcgtag gttaattaaa
     2641 aacgcgtatt agcttccgct agacaaatgt gattaccccc gctagacaaa tgtgattacc
     2701 cgcgctagac aaatgtgatt accccgctag acaaatgtga ttaccccccg ctagacaaat
     2761 gtgattaccc ccgctagaca aatgtgatta cccgcgctag acaaatgtga ttaccccgct
     2821 agacaaatgt gattaccccc gaccagggca taaatggcca ggtgggacca gagatgctac
     2881 cggactcaga tctcgccacc atgctgcaga acgagcttgc tcttaagttg gctggacttg
     2941 atattaacaa gactggagga ggttctcatc atcatcatca tcatggtatg gctagcatga
     3001 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ctcgcaacaa
     3061 tggtcgactc atcacgtcgt aagtggaata aggcaggtca cgcagtcaga gctataggtc
     3121 ggctgagctc acccgtggtt tccgagcgga tgtaccccga ggacggcgcc ctgaagagcg
     3181 agatcaagaa ggggctgagg ctgaaggacg gcggccacta cgccgccgag gtcaagacca
     3241 cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtggac atcaagttgg
     3301 acatcgtgtc ccacaacgag gactacacca tcgtggaaca gtgcgaacgc gccgagggcc
     3361 gccactccac cggcggcatg gacgagctgt acaagggagg tacaggcggg agtctggtga
     3421 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     3481 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3541 acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     3601 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacattaag cacccagccg
     3661 acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga
     3721 acttcgagga cggcggcatt attcacgtga accaggactc ctccctgcag gacggcgtat
     3781 tcatctacaa ggtgaagctg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     3841 agaagaccat gggctgggag gctacgcgtg acgacctgac tgaagagcag atcgcagaat
     3901 ttaaagaggc tttctcccta tttgacaagg acggggatgg gacgataaca accaaggagc
     3961 ttgggacggt gttccgatcg ctggggcaga accccacaga agcagagctg caggacatga
     4021 tcaatgaagt agatgccgac ggtgacggca cattcgactt ccctgagttc ctgacgatga
     4081 tggcaagaaa aatgaatgac acagacagtg aagaggaaat tcgcgaagcg ttccgcgtgt
     4141 ttgataagga cggcaatggc tacatcggcg cagcagagct tcgccacgtg atgacagacc
     4201 ttggagagaa gttaacagat gaggaggttg atgaaatgat cagggtagca gacatcgatg
     4261 gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag taggcggcca
     4321 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4741 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     4921 tcgataccgt cgacccgggc ggccgctcat aaagctcgac tgtgccttct agttgccagc
     4981 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5041 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5101 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5161 ctggggatgc ggtccggact gtacaattgc attcatttta tgtttcaggt tcagggggag
     5221 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     5281 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga
     5341 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctg
//
