LOCUS       sequence-415670-       11986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and mutations in the P loop of Domains I, II, 
            III and IV which abolish trafficking to the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A,...
FEATURES             Location/Qualifiers
     source          1..11986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3468..3494
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3498..3524
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9004..9023)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9069..9124
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9123..9142)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9485..9501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9485..9501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9498..9520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9509..9525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9533..9539,9540..9557,9558..9563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9578..9599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9658..9853
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9704..9839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9859..9993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9896..9915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9950..9969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10061..10078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10232..10820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10312..10331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10991..11782,11783..11851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11614..11633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11852..11956)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagggtacc
     1741 gggccccccc tcgaggtcga agcttgatca tggtccgctt cggggacgag ctgggcggcc
     1801 gctatggggg cgcgggcggc gcggagcggg cccggggcgg cggggccggc ggggctggcg
     1861 gcccgggccc cggggggctg ccgccgggcc agcgggtcct ctacaaacag tcgatcgcgc
     1921 agcgcgcgcg gaccatggcg ctgtacaacc ccatcccggt caagcagaac tgcttcaccg
     1981 tcaaccgctc gctcttcgtc ttcagcgagg acaacgtcgt ccgcaaatac gccaagcgca
     2041 tcaccgagtg gcctccgttc gagtacatga tcctggccac catcatcgcc aactgcatcg
     2101 tgctggccct ggagcagcac ctccctgacg gggacaagac gcctatgtcc gagcgcctgg
     2161 atgacaccga gccctacttc attgggatct tctgcttcga ggcggggatc aagatcctgg
     2221 ccctgggctt tgttctgcac aagggctcct atctgcggaa cggctggaac gtcatggact
     2281 tcgtggtagt cctcacgggg attcttgcca cagccggaac cgacttcgac ctgcgaacgc
     2341 tgagggctgt gcgtgtgcta aggcccctga agctggtgtc tgggattccg agcttgcaag
     2401 tggtgctcaa gtccatcatg aaggccatgg tcccgctgct gcagatcggg ctgctgctct
     2461 tcttcgccat cctcatgttt gccatcatcg gcctcgagtt ctacatgggc aagttccaca
     2521 aggcctgctt ccccaacagc acagacccag atcccgtggg tgacttcccc tgtggcaagg
     2581 aggccccggc ccggctctgt gagggagaca cggagtgccg ggagtactgg gccggaccca
     2641 actttggtat caccaacttt gacaacatcc tgtttgccat cttgaccgtg ttccagtgca
     2701 tcaccatggc gggctggact gacatcctct acaatacaaa cgatgcagcc ggcaacacgt
     2761 ggaactggct ctacttcatc cctctcatca tcatcggctc cttcttcatg ctcaacctgg
     2821 tgctgggtgt gctctcagga gagtttgcta aggagcgcga gagagtggag aaccgccgcg
     2881 ccttcctgaa gctccggcgg cagcagcaga ttgagcggga gctcaacggg tacctggagt
     2941 ggatcttcaa ggctgaggaa gtcatgctgg ctgaggagga caggaacgca gaggagaagt
     3001 cccctctgga tgcagtgttg aagagagcag ctgccaagaa gagcagaagc gacctgatcc
     3061 aggccgagga gggggagggc cggctcacag gcctctgtgc tcccggttcc ccgttcgccc
     3121 gtgccagcct gaaaagcggg aagacggaga gctcgtcgta cttccggagg aaggagaaga
     3181 tgttccgctt cttcatccgg cgcatggtga aggcacagag cttctactgg acggtgctgt
     3241 gtgtggtggc actgaacaca ctgtgcgtgg ccatggtgca ctacaaccag ccgcagcggc
     3301 tcaccacggc attgtacttt gcagagtttg ttttcctggg tctcttcctc acagagatgt
     3361 ccctgaagat gtatggcctg gggcccagga gctacttccg gtcctccttc aactgttttg
     3421 actttggggt catcgtgggc agtatctttg aagtggtctg ggctgcttac ccctacgacg
     3481 tccccgacta cgccggctac ccgtacgacg tcccggacta cgccggcgtc gacgtcaagc
     3541 cagggacctc cttcgggatc agtgttctgc gggctctgcg cttactgaga atcttcaagg
     3601 tcaccaaata ctggaactcc ctacggaacc tggtggtgtc tctgctcaac tccatgaagt
     3661 ccatcatcag cctcctcttc ctgctcttcc tgttcattgt tgtctttgct ctgctgggga
     3721 tgcagctgtt tggaggccag ttcaacttta aagacgagac cccaacgacc aactttgaca
     3781 ccttccctgc tgctatcctc accgtcttcc agatcctgac cggagcggac tggaatgcgg
     3841 tcatgtacca cgggatcgag tcgcaaggtg gcgtcagcag aggaatgttc tcctccttct
     3901 acttcattgt cctcactctg tttgggaact acaccctgct gaatgtcttc ctggccattg
     3961 ctgtggacaa ccttgccaat gctcaggagc tgaccaagga tgaagaggag atggaagaag
     4021 cagccaatca gaaactcgcc ttgcagaagg ccaaggaagt ggctgaagtc agccccatgt
     4081 ctgctgcgaa catctccatt gccgccaggc agcagaactc ggccaaagcg cgctcggtgt
     4141 gggagcagcg agccagccag ctgcggctgc agaacctgag ggccagctgc gaggcgctgt
     4201 acagcgagat ggaccccgag gagaggctgc gctacgccac cgcgcgccac ctgcggcccg
     4261 acgtgaagac gcacctggac cggccgctgg tggttgagcc cggccgcgac gctccgcggg
     4321 gccccccggg ggggaagtcc cggccggatg gctcggaggc ccccgagggt gcggatcccc
     4381 cgcgcaggca tcaccggcac cgagacaagg acaaggctcc cgccacggtc ccctcggcgg
     4441 gggaacagga cagagccgaa gctctgaggg cagagggcgg ggagctgggg ccccgcgagg
     4501 agcgggggcg gccacgccgc agccgcagca aggaggcccc gggcgccccg gaggtgcgca
     4561 gcgatcgcgg ccgtggcccg tgtcctgaag gtggtcggcg ccaccaccgg cgcgggtcac
     4621 cggaggaggc agctgagagg gagccccgcc gccaccgcgc gcaccggcac gggcccgacc
     4681 cgggcaagga gggcccagcg tccgggacca ggggcgagcg gcgcgcacga caccgcacgg
     4741 gcccccgggc gtgcccccgg gaggctgaga gcagcgagga gcctgcgcgg cggcaccggg
     4801 ctcggcacaa ggcgccgccc acccaggaga ccgcggagaa ggacaaggag gctgccgaga
     4861 aggggggcga ggccacggag gccgagaagg acaaggaggc tcggaaccac cagcccaagg
     4921 agctgccctg tgacctggag gccatcggca tgctgggagt gggcgctgtg catacgctgc
     4981 ccagcacctg tctgcagaag gtggaggagc agccagagga tgcggacaac cagcggaacg
     5041 tcacccgcat gggtagtcag cccccggaca ccagcaccac tgtgcacatt cctgtgacgc
     5101 tgaccggccc ccccggggag accacggtag tgcccagtgg aaacgtggac ctggaaagtc
     5161 aagcagaggg gaagaaggag gtggaaacca gcgatgtgat gaggagcggc cctcggccca
     5221 ttgtcccgta cagttccatg ttctgtttaa gcccaaccaa cctgctgcgc cgctgctgcc
     5281 actacattgt gaccatgcga tacttcgaga tggtcatcct cgtggtcatc gccctgagca
     5341 gcatcgccct ggctgccgag gaccccgtga gaacagactc tcccaggaat aatgctctga
     5401 agtacatgga ctacatcttc acaggcgtct tcaccttcga gatggtgata aagatgatcg
     5461 atttgggact gctgctacac cctggagcct atttccggga tctgtggaac attctggact
     5521 tcattgtggt cagtggggcc ctggtggcat ttgctttctc aggatccaaa gggaaagaca
     5581 tcagcaccat caaatccctg agagtcctgc gtgtgctgcg gccactcaag accatcaagc
     5641 ggctgcccaa gctcaaggcc gtgtttgact gtgtggtgaa ctcactgaaa aacgtcctca
     5701 acatcctgat cgtctacatg ctcttcatgt tcatctttgc cgtcattgcc gtgcagctct
     5761 tcaaagggaa gttcttctac tgcacggacg aatccaagga gctggagcgt gactgcaggg
     5821 gtcagtattt ggattatgag aaggaggaag tggaagctca accgaggcag tggaagaaat
     5881 atgacttcca ctatgacaac gtgctctggg ctctgctaac gctcttcacg gtgtccacag
     5941 gggcagggtg gcccatggtg ctgaaacact ctgtggatgc cacctacgag gagcagggcc
     6001 cgagccccgg gtaccgcatg gagctctcca tcttctacgt ggtctacttt gtggtctttc
     6061 ccttcttctt tgtcaacatc ttcgtggcct tgatcatcat caccttccag gagcaggggg
     6121 acaaggtgat gtccgagtgc agcctggaga agaacgagag ggcttgcatc gactttgcca
     6181 tcagcgccag acccctgacg cggtacatgc cccagaacaa gcagtcgttc cagtataaga
     6241 cgtggacgtt cgtggtgtcc ccaccttttg agtacttcat catggctatg atcgccctca
     6301 acactgtggt gctgatgatg aagttctacg atgcgccgta cgagtatgag ctaatgctga
     6361 aatgcctgaa catcgtgttc acgtccatgt tctccatgga gtgtgtgctg aagatcatcg
     6421 ccttcggggt gctgaactat ttcagagatg cctggaatgt ctttgacttt gtcacggtgt
     6481 tgggaagtat tactgatatt ttggtaacag agattgcgaa caacttcatc aacctcagct
     6541 tcctgcgcct gttccgggcc gcgcggctca tcaagctgct gcgccagggc tacaccatcc
     6601 gcatcctgct ttggaccttc gtgcagtcct tcaaggcgct gccctacgtg tgtctcctca
     6661 tcgccatgct cttcttcatc tacgccatca ttggcatgca ggtttttggg aacatcgccc
     6721 tggacgatga caccagcatc aacaggcaca ataacttccg gacgtttctg caggccttga
     6781 tgttgctgtt caggagcgcc acgggggcgg cctggcacga gataatgctg tcgtgcctca
     6841 gcagccgggc ctgcgacgag cactccaatg ccagcgagtg cggcagcgac tttgcctact
     6901 tttacttcgt ctccttcatc ttcctctgct cctttctgat gttgaacctc tttgtggctg
     6961 tgatcatgga caattttgag taccttacac gggactcttc catcttaggg cctcaccacc
     7021 tggatgagtt catccgggtc tgggctgaat acgacccggc cgcgtgtggg cgcatcagtt
     7081 acagtgacat gtttgagatg ctgaagcaca tgtccccacc cctggggctg gggaagaagt
     7141 gccctgctcg agtcgcgtac aagcgcctgg tccgcatgaa catgcccatc tccagtgagg
     7201 acatgacggt gcacttcaca tccacgctga tggctctcat tcggacagcg ctggacatca
     7261 agctggcacc agctgggacc aagcagcacc agtgcgacgc agagctgagg aaggagattt
     7321 cctgtgtgtg ggccaatctg ccccagaaga ccctggactt actggtacca ccccataagc
     7381 ctgacgagat gacggtgggc aaagtctatg cagctctgat gatcttcgac ttctacaaac
     7441 agaacaaaac cagcagagac cagactcagc aagcccccgg aggcctgtcc cagctgggcc
     7501 ccgtgtccct gttccacccg ctgaaggcca cgctggagca gactcagccg gcgctgcgtg
     7561 gagcccgggc tttcctgcgg cagaagagtt ctgcctccct gagcaacggc ggggccgtac
     7621 aaacccagga gagcggcatc aaggaatcgg tttcttgggg cactcagagg acccaggatg
     7681 tgctttgcga ggccagggca cccctggagc ggggccactc agctgagatc ccagtcgggc
     7741 agcctgggac actggctgta gatgtccaga tgcagaacat gactctgagt ggccctgacg
     7801 ccgagccgca gccagggctg gagagccagg gccgagccgc ctccatgccc cgcctggccg
     7861 cagaaacaca gcccgcgcct gacgccagcc ccatgaagcg ctccatctcc acgctggccc
     7921 cgcgccccca cacggcccgg ctcggcagca ccgcgctgga ccgcccagcc cccagccagg
     7981 ccccgcacca tcaccaccac cgctgccacc gccgcaggga caggaagcag cggtccctgg
     8041 agaagggtcc cagcctgtct gctgacacag atggggcccc cgacagcacg gtggggccag
     8101 gactgcccac gggggagggc ccccccggct gcaggcggga gcgggagcgc aggcaggagc
     8161 ggggccggtc ccaggagcgg aggcagccct cgtcctcctc ctcggagaag catcgcttct
     8221 actcctgtga ccgcttcggg ggccgcgaac ccccacagcc caagccctcc ctcagtagtc
     8281 accccacgtc gccgacggcc gggcaggagc cgggacccca cccgcagggg agtggttcgg
     8341 ttcacgggag ccccctgctg tccacatctg gtgctagcac cccgggccgg gggcggaggc
     8401 agctccccca gacccccttg accccccgcc ccagcgtcac ctacaagacg gccaactcct
     8461 cgcctgtcca cttcgccggg gcaccgagcg gcctccccgc cttctccccc ggccggctca
     8521 gccggggcct ctcagaacac aacgccctgc tgcagaggga cccgctcagc cggcccctgg
     8581 cgcccggctc gcgcatcggc tccgacccct acctggggca gcggctggac agcgaggcgc
     8641 ccgcccgtgc cctgcccgag gacgcgcccg cctttgagga gaccgcggcc agcaactcgg
     8701 gccgctcctc ccggacctcg tacgtgtcct cactgacctc gcagcccccg ccgctgcgcc
     8761 gcgtgcccaa cggctaccac tgcactctgg ggctgggcgg gggcggccgg gcgcggcgcg
     8821 gctgccacca ccccgaccgc gaccgccggt gctagactag ttctagcaag cttcgacgaa
     8881 ttcgcatgcg gccgctagct cgaggtaccg atatcgagct caattcactc ctcaggtgca
     8941 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     9001 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     9061 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     9121 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     9181 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     9241 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     9301 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     9361 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     9421 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     9481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     9661 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9721 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9781 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9841 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9901 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9961 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
    10021 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10081 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10141 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10201 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10801 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10861 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    11101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11281 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11581 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11881 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11941 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtc
//
