LOCUS       sequence-415673-       13302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal GFP 
            tag and an exofacial double HA tag in domain II and a D122A mutation
            that disrupts the interaction with ?2?-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 13302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: GFP Cav2.2 HA D122A pcDNA3
FEATURES             Location/Qualifiers
     source          1..13302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     158..177
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    462..583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(499..518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     553..572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(632..648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(632..648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(645..667)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    656..672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(680..686,687..704,705..710))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    725..746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(863..880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1034..1622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1114..1133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1793..2584,2585..2653))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2416..2435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2654..2758)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2833..2852)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3024..3403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3404..3607
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3558..3578
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3652..3671
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3652..3670
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3762..4475
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3762..4475
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3792..3820)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4415..4435
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             6174..6200
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6204..6230
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(11644..11662)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11645..11662)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11682..11699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11688..11912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11958..12386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12045..12064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12255..12276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12395..12415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12400..12729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12580..12715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12642..12661
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(12796..13302,1..288)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12850..12869)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
       61 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
      121 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
      181 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
      241 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
      301 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
      361 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
      421 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
      481 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      541 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
      601 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2761 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     2821 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     2881 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     2941 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     3001 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     3061 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     3121 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     3181 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     3241 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     3301 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     3361 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     3421 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     3481 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     3541 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     3601 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
     3661 tcactatagg gagacccaag cttggtaccg agctcggatc cactagtaac ggccgccagt
     3721 gtgctggaat tcctcgaggg taccgagctc ggatctaaaa aatgagtaaa ggagaagaac
     3781 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     3841 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     3901 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
     3961 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     4021 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     4081 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     4141 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     4201 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     4261 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     4321 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     4381 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     4441 ctgggattac acatggcatg gatgaactat acaaaatggt ccgcttcggg gacgagctgg
     4501 gcggccgcta tgggggcgcg ggcggcgcgg agcgggcccg gggcggcggg gccggcgggg
     4561 ctggcggccc gggccccggg gggctgccgc cgggccagcg ggtcctctac aaacagtcga
     4621 tcgcgcagcg cgcgcggacc atggcgctgt acaaccccat cccggtcaag cagaactgct
     4681 tcaccgtcaa ccgctcgctc ttcgtcttca gcgaggacaa cgtcgtccgc aaatacgcca
     4741 agcgcatcac cgagtggcct ccgttcgagt acatgatcct ggccaccatc atcgccaact
     4801 gcatcgtgct ggccctggag cagcacctcc ctgacggggc caagacgcct atgtccgagc
     4861 gcctggatga caccgagccc tacttcattg ggatcttctg cttcgaggcg gggatcaaga
     4921 tcctggccct gggctttgtt ctgcacaagg gctcctatct gcggaacggc tggaacgtca
     4981 tggacttcgt ggtagtcctc acggggattc ttgccacagc cggaaccgac ttcgacctgc
     5041 gaacgctgag ggctgtgcgt gtgctaaggc ccctgaagct ggtgtctggg attccgagct
     5101 tgcaagtggt gctcaagtcc atcatgaagg ccatggtccc gctgctgcag atcgggctgc
     5161 tgctcttctt cgccatcctc atgtttgcca tcatcggcct cgagttctac atgggcaagt
     5221 tccacaaggc ctgcttcccc aacagcacag acccagatcc cgtgggtgac ttcccctgtg
     5281 gcaaggaggc cccggcccgg ctctgtgagg gagacacgga gtgccgggag tactgggccg
     5341 gacccaactt tggtatcacc aactttgaca acatcctgtt tgccatcttg accgtgttcc
     5401 agtgcatcac catggagggc tggactgaca tcctctacaa tacaaacgat gcagccggca
     5461 acacgtggaa ctggctctac ttcatccctc tcatcatcat cggctccttc ttcatgctca
     5521 acctggtgct gggtgtgctc tcaggagagt ttgctaagga gcgcgagaga gtggagaacc
     5581 gccgcgcctt cctgaagctc cggcggcagc agcagattga gcgggagctc aacgggtacc
     5641 tggagtggat cttcaaggct gaggaagtca tgctggctga ggaggacagg aacgcagagg
     5701 agaagtcccc tctggatgca gtgttgaaga gagcagctgc caagaagagc agaagcgacc
     5761 tgatccaggc cgaggagggg gagggccggc tcacaggcct ctgtgctccc ggttccccgt
     5821 tcgcccgtgc cagcctgaaa agcgggaaga cggagagctc gtcgtacttc cggaggaagg
     5881 agaagatgtt ccgcttcttc atccggcgca tggtgaaggc acagagcttc tactggacgg
     5941 tgctgtgtgt ggtggcactg aacacactgt gcgtggccat ggtgcactac aaccagccgc
     6001 agcggctcac cacggcattg tactttgcag agtttgtttt cctgggtctc ttcctcacag
     6061 agatgtccct gaagatgtat ggcctggggc ccaggagcta cttccggtcc tccttcaact
     6121 gttttgactt tggggtcatc gtgggcagta tctttgaagt ggtctgggct gcttacccct
     6181 acgacgtccc cgactacgcc ggctacccgt acgacgtccc ggactacgcc ggcgtcgacg
     6241 tcaagccagg gacctccttc gggatcagtg ttctgcgggc tctgcgctta ctgagaatct
     6301 tcaaggtcac caaatactgg aactccctac ggaacctggt ggtgtctctg ctcaactcca
     6361 tgaagtccat catcagcctc ctcttcctgc tcttcctgtt cattgttgtc tttgctctgc
     6421 tggggatgca gctgtttgga ggccagttca actttaaaga cgagacccca acgaccaact
     6481 ttgacacctt ccctgctgct atcctcaccg tcttccagat cctgaccgga gaggactgga
     6541 atgcggtcat gtaccacggg atcgagtcgc aaggtggcgt cagcagagga atgttctcct
     6601 ccttctactt cattgtcctc actctgtttg ggaactacac cctgctgaat gtcttcctgg
     6661 ccattgctgt ggacaacctt gccaatgctc aggagctgac caaggatgaa gaggagatgg
     6721 aagaagcagc caatcagaaa ctcgccttgc agaaggccaa ggaagtggct gaagtcagcc
     6781 ccatgtctgc tgcgaacatc tccattgccg ccaggcagca gaactcggcc aaagcgcgct
     6841 cggtgtggga gcagcgagcc agccagctgc ggctgcagaa cctgagggcc agctgcgagg
     6901 cgctgtacag cgagatggac cccgaggaga ggctgcgcta cgccaccgcg cgccacctgc
     6961 ggcccgacgt gaagacgcac ctggaccggc cgctggtggt tgagcccggc cgcgacgctc
     7021 cgcggggccc cccggggggg aagtcccggc cggatggctc ggaggccccc gagggtgcgg
     7081 atcccccgcg caggcatcac cggcaccgag acaaggacaa ggctcccgcc acggtcccct
     7141 cggcggggga acaggacaga gccgaagctc tgagggcaga gggcggggag ctggggcccc
     7201 gcgaggagcg ggggcggcca cgccgcagcc gcagcaagga ggccccgggc gccccggagg
     7261 tgcgcagcga tcgcggccgt ggcccgtgtc ctgaaggtgg tcggcgccac caccggcgcg
     7321 ggtcaccgga ggaggcagct gagagggagc cccgccgcca ccgcgcgcac cggcacgggc
     7381 ccgacccggg caaggagggc ccagcgtccg ggaccagggg cgagcggcgc gcacgacacc
     7441 gcacgggccc ccgggcgtgc ccccgggagg ctgagagcag cgaggagcct gcgcggcggc
     7501 accgggctcg gcacaaggcg ccgcccaccc aggagaccgc ggagaaggac aaggaggctg
     7561 ccgagaaggg gggcgaggcc acggaggccg agaaggacaa ggaggctcgg aaccaccagc
     7621 ccaaggagct gccctgtgac ctggaggcca tcggcatgct gggagtgggc gctgtgcata
     7681 cgctgcccag cacctgtctg cagaaggtgg aggagcagcc agaggatgcg gacaaccagc
     7741 ggaacgtcac ccgcatgggt agtcagcccc cggacaccag caccactgtg cacattcctg
     7801 tgacgctgac cggccccccc ggggagacca cggtagtgcc cagtggaaac gtggacctgg
     7861 aaagtcaagc agaggggaag aaggaggtgg aaaccagcga tgtgatgagg agcggccctc
     7921 ggcccattgt cccgtacagt tccatgttct gtttaagccc aaccaacctg ctgcgccgct
     7981 gctgccacta cattgtgacc atgcgatact tcgagatggt catcctcgtg gtcatcgccc
     8041 tgagcagcat cgccctggct gccgaggacc ccgtgagaac agactctccc aggaataatg
     8101 ctctgaagta catggactac atcttcacag gcgtcttcac cttcgagatg gtgataaaga
     8161 tgatcgattt gggactgctg ctacaccctg gagcctattt ccgggatctg tggaacattc
     8221 tggacttcat tgtggtcagt ggggccctgg tggcatttgc tttctcagga tccaaaggga
     8281 aagacatcag caccatcaaa tccctgagag tcctgcgtgt gctgcggcca ctcaagacca
     8341 tcaagcggct gcccaagctc aaggccgtgt ttgactgtgt ggtgaactca ctgaaaaacg
     8401 tcctcaacat cctgatcgtc tacatgctct tcatgttcat ctttgccgtc attgccgtgc
     8461 agctcttcaa agggaagttc ttctactgca cggacgaatc caaggagctg gagcgtgact
     8521 gcaggggtca gtatttggat tatgagaagg aggaagtgga agctcaaccg aggcagtgga
     8581 agaaatatga cttccactat gacaacgtgc tctgggctct gctaacgctc ttcacggtgt
     8641 ccacagggga agggtggccc atggtgctga aacactctgt ggatgccacc tacgaggagc
     8701 agggcccgag ccccgggtac cgcatggagc tctccatctt ctacgtggtc tactttgtgg
     8761 tctttccctt cttctttgtc aacatcttcg tggccttgat catcatcacc ttccaggagc
     8821 agggggacaa ggtgatgtcc gagtgcagcc tggagaagaa cgagagggct tgcatcgact
     8881 ttgccatcag cgccagaccc ctgacgcggt acatgcccca gaacaagcag tcgttccagt
     8941 ataagacgtg gacgttcgtg gtgtccccac cttttgagta cttcatcatg gctatgatcg
     9001 ccctcaacac tgtggtgctg atgatgaagt tctacgatgc gccgtacgag tatgagctaa
     9061 tgctgaaatg cctgaacatc gtgttcacgt ccatgttctc catggagtgt gtgctgaaga
     9121 tcatcgcctt cggggtgctg aactatttca gagatgcctg gaatgtcttt gactttgtca
     9181 cggtgttggg aagtattact gatattttgg taacagagat tgcgaacaac ttcatcaacc
     9241 tcagcttcct gcgcctgttc cgggccgcgc ggctcatcaa gctgctgcgc cagggctaca
     9301 ccatccgcat cctgctttgg accttcgtgc agtccttcaa ggcgctgccc tacgtgtgtc
     9361 tcctcatcgc catgctcttc ttcatctacg ccatcattgg catgcaggtt tttgggaaca
     9421 tcgccctgga cgatgacacc agcatcaaca ggcacaataa cttccggacg tttctgcagg
     9481 ccttgatgtt gctgttcagg agcgccacgg gggaggcctg gcacgagata atgctgtcgt
     9541 gcctcagcag ccgggcctgc gacgagcact ccaatgccag cgagtgcggc agcgactttg
     9601 cctactttta cttcgtctcc ttcatcttcc tctgctcctt tctgatgttg aacctctttg
     9661 tggctgtgat catggacaat tttgagtacc ttacacggga ctcttccatc ttagggcctc
     9721 accacctgga tgagttcatc cgggtctggg ctgaatacga cccggccgcg tgtgggcgca
     9781 tcagttacag tgacatgttt gagatgctga agcacatgtc cccacccctg gggctgggga
     9841 agaagtgccc tgctcgagtc gcgtacaagc gcctggtccg catgaacatg cccatctcca
     9901 gtgaggacat gacggtgcac ttcacatcca cgctgatggc tctcattcgg acagcgctgg
     9961 acatcaagct ggcaccagct gggaccaagc agcaccagtg cgacgcagag ctgaggaagg
    10021 agatttcctg tgtgtgggcc aatctgcccc agaagaccct ggacttactg gtaccacccc
    10081 ataagcctga cgagatgacg gtgggcaaag tctatgcagc tctgatgatc ttcgacttct
    10141 acaaacagaa caaaaccagc agagaccaga ctcagcaagc ccccggaggc ctgtcccagc
    10201 tgggccccgt gtccctgttc cacccgctga aggccacgct ggagcagact cagccggcgc
    10261 tgcgtggagc ccgggctttc ctgcggcaga agagttctgc ctccctgagc aacggcgggg
    10321 ccgtacaaac ccaggagagc ggcatcaagg aatcggtttc ttggggcact cagaggaccc
    10381 aggatgtgct ttgcgaggcc agggcacccc tggagcgggg ccactcagct gagatcccag
    10441 tcgggcagcc tgggacactg gctgtagatg tccagatgca gaacatgact ctgagtggcc
    10501 ctgacgccga gccgcagcca gggctggaga gccagggccg agccgcctcc atgccccgcc
    10561 tggccgcaga aacacagccc gcgcctgacg ccagccccat gaagcgctcc atctccacgc
    10621 tggccccgcg cccccacacg gcccggctcg gcagcaccgc gctggaccgc ccagccccca
    10681 gccaggcccc gcaccatcac caccaccgct gccaccgccg cagggacagg aagcagcggt
    10741 ccctggagaa gggtcccagc ctgtctgctg acacagatgg ggcccccgac agcacggtgg
    10801 ggccaggact gcccacgggg gagggccccc ccggctgcag gcgggagcgg gagcgcaggc
    10861 aggagcgggg ccggtcccag gagcggaggc agccctcgtc ctcctcctcg gagaagcatc
    10921 gcttctactc ctgtgaccgc ttcgggggcc gcgaaccccc acagcccaag ccctccctca
    10981 gtagtcaccc cacgtcgccg acggccgggc aggagccggg accccacccg caggggagtg
    11041 gttcggttca cgggagcccc ctgctgtcca catctggtgc tagcaccccg ggccgggggc
    11101 ggaggcagct cccccagacc cccttgaccc cccgccccag cgtcacctac aagacggcca
    11161 actcctcgcc tgtccacttc gccggggcac cgagcggcct ccccgccttc tcccccggcc
    11221 ggctcagccg gggcctctca gaacacaacg ccctgctgca gagggacccg ctcagccggc
    11281 ccctggcgcc cggctcgcgc atcggctccg acccctacct ggggcagcgg ctggacagcg
    11341 aggcgcccgc ccgtgccctg cccgaggacg cgcccgcctt tgaggagacc gcggccagca
    11401 actcgggccg ctcctcccgg acctcgtacg tgtcctcact gacctcgcag cccccgccgc
    11461 tgcgccgcgt gcccaacggc taccactgca ctctggggct gggcgggggc ggccgggcgc
    11521 ggcgcggctg ccaccacccc gaccgcgacc gccggtgcta gactagttct agcaagcttc
    11581 gacgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc
    11641 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
    11701 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
    11761 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
    11821 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
    11881 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
    11941 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    12001 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    12061 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    12121 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    12181 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    12241 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    12301 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    12361 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
    12421 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    12481 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    12541 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    12601 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    12661 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    12721 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
    12781 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
    12841 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
    12901 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
    12961 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
    13021 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
    13081 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
    13141 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
    13201 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
    13261 gatgatctgg acgaagagca tcaggggctc gcgccagccg aa
//
