LOCUS       sequence-415674-       14422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BCR-ABL WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Sokirniy I, Inam H, Tomaszkiewicz M, Reynolds J, McCandlish D, 
            Pritchard J
  TITLE     A side-by-side comparison of variant function measurements using 
            deep mutational scanning and base editing.
  JOURNAL   Nucleic Acids Res. 2025 Jul 19;53(14):gkaf738. doi: 
            10.1093/nar/gkaf738.
  PUBMED    40744495
REFERENCE   2  (bases 1 to 14422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-07-19"; volume: "53(14):gkaf738. doi"; 
            pages: " 10.1093/nar/gkaf738"
COMMENT     Sequence Label: pUltra BCR-ABL WT
FEATURES             Location/Qualifiers
     source          1..14422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2022..3233
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3070..3089
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3266..3275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3272..3274,3275..3277,3278..3988)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3272..3274,3275..3277,3278..3988)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3317..3338)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3578..3597)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3925..3946
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3998..4054
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10172..10225
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10269..10857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10322..10342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10740..10751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10948..11181
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11253..11374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11290..11309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11344..11363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11414..11549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11569..11588)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11570..11588)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11598..11615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11598..11614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11607..11629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11756..12211
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11843..11862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12053..12074
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12237..12341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12342..12410,12411..13202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12560..12579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13373..13961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13862..13881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14115..14132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14249..14270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14285..14290,14291..14308,14309..14315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14323..14339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14328..14350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14347..14363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14347..14363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14382..14402
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14384..14402
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     1801 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1981 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2041 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2101 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2161 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2221 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2281 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2341 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     2401 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     2461 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     2521 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     2581 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     2641 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     2701 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     2761 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     2821 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     2881 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     2941 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3001 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3061 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3121 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3181 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3241 gggctgcagg tccgatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     3301 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3361 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3421 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3481 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3541 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3601 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3661 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3721 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3781 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3841 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3901 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3961 cactctcggc atggacgagc tgtacaaggg atctggagca acaaacttct cactactcaa
     4021 acaagcaggt gacgtggagg agaatcccgg gcctatggtg gacccggtgg gcttcgcgga
     4081 ggcgtggaag gcgcagttcc cggactcaga gcccccgcgc atggagctgc gctcagtggg
     4141 cgacatcgag caggagctgg agcgctgcaa ggcctccatt cggcgcctgg agcaggaggt
     4201 gaaccaggag cgcttccgca tgatctacct gcagacgttg ctggccaagg aaaagaagag
     4261 ctatgaccgg cagcgatggg gcttccggcg cgcggcgcag gcccccgacg gcgcctccga
     4321 gccccgagcg tccgcgtcgc gcccgcagcc agcgcccgcc gacggagccg acccgccgcc
     4381 cgccgaggag cccgaggccc ggcccgacgg cgagggttct ccgggtaagg ccaggcccgg
     4441 gaccgcccgc aggcccgggg cagccgcgtc gggggaacgg gacgaccggg gaccccccgc
     4501 cagcgtggcg gcgctcaggt ccaacttcga gcggatccgc aagggccatg gccagcccgg
     4561 ggcggacgcc gagaagccct tctacgtgaa cgtcgagttt caccacgagc gcggcctggt
     4621 gaaggtcaac gacaaagagg tgtcggaccg catcagctcc ctgggcagcc aggccatgca
     4681 gatggagcgc aaaaagtccc agcacggcgc gggctcgagc gtgggggatg catccaggcc
     4741 cccttaccgg ggacgctcct cggagagcag ctgcggcgtc gacggcgact acgaggacgc
     4801 cgagttgaac ccccgcttcc tgaaggacaa cctgatcgac gccaatggcg gtagcaggcc
     4861 cccttggccg cccctggagt accagcccta ccagagcatc tacgtcgggg gcatgatgga
     4921 aggggagggc aagggcccgc tcctgcgcag ccagagcacc tctgagcagg agaagcgcct
     4981 tacctggccc cgcaggtcct actccccccg gagttttgag gattgcggag gcggctatac
     5041 cccggactgc agctccaatg agaacctcac ctccagcgag gaggacttct cctctggcca
     5101 gtccagccgc gtgtccccaa gccccaccac ctaccgcatg ttccgggaca aaagccgctc
     5161 tccctcgcag aactcgcaac agtccttcga cagcagcagt ccccccacgc cgcagtgcca
     5221 taagcggcac cggcactgcc cggttgtcgt gtccgaggcc accatcgtgg gcgtccgcaa
     5281 gaccgggcag atctggccca acgatggcga gggcgccttc catggagacg cagatggctc
     5341 gttcggaaca ccacctggat acggctgcgc tgcagaccgg gcagaggagc agcgccggca
     5401 ccaagatggg ctgccctaca ttgatgactc gccctcctca tcgccccacc tcagcagcaa
     5461 gggcaggggc agccgggatg cgctggtctc gggagccctg gagtccacta aagcgagtga
     5521 gctggacttg gaaaagggct tggagatgag aaaatgggtc ctgtcgggaa tcctggctag
     5581 cgaggagact tacctgagcc acctggaggc actgctgctg cccatgaagc ctttgaaagc
     5641 cgctgccacc acctctcagc cggtgctgac gagtcagcag atcgagacca tcttcttcaa
     5701 agtgcctgag ctctacgaga tccacaagga gttctatgat gggctcttcc cccgcgtgca
     5761 gcagtggagc caccagcagc gggtgggcga cctcttccag aagctggcca gccagctggg
     5821 tgtgtaccgg gccttcgtgg acaactacgg agttgccatg gaaatggctg agaagtgctg
     5881 tcaggccaat gctcagtttg cagaaatctc cgagaacctg agagccagaa gcaacaaaga
     5941 tgccaaggat ccaacgacca agaactctct ggaaactctg ctctacaagc ctgtggaccg
     6001 tgtgacgagg agcacgctgg tcctccatga cttgctgaag cacactcctg ccagccaccc
     6061 tgaccacccc ttgctgcagg acgccctccg catctcacag aacttcctgt ccagcatcaa
     6121 tgaggagatc acaccccgac ggcagtccat gacggtgaag aagggagagc accggcagct
     6181 gctgaaggac agcttcatgg tggagctggt ggagggggcc cgcaagctgc gccacgtctt
     6241 cctgttcacc gacctgcttc tctgcaccaa gctcaagaag cagagcggag gcaaaacgca
     6301 gcagtatgac tgcaaatggt acattccgct cacggatctc agcttccaga tggtggatga
     6361 actggaggca gtgcccaaca tccccctggt gcccgatgag gagctggacg ctttgaagat
     6421 caagatctcc cagatcaaga atgacatcca gagagagaag agggcgaaca agggcagcaa
     6481 ggctacggag aggctgaaga agaagctgtc ggagcaggag tcactgctgc tgcttatgtc
     6541 tcccagcatg gccttcaggg tgcacagccg caacggcaag agttacacgt tcctgatctc
     6601 ctctgactat gagcgtgcag agtggaggga gaacatccgg gagcagcaga agaagtgttt
     6661 cagaagcttc tccctgacat ccgtggagct gcagatgctg accaactcgt gtgtgaaact
     6721 ccagactgtc cacagcattc cgctgaccat caataaggaa gatgatgagt ctccggggct
     6781 ctatgggttt ctgaatgtca tcgtccactc agccactgga tttaagcaga gttcaaaagc
     6841 ccttcagcgg ccagtagcat ctgactttga gcctcagggt ctgagtgaag ccgctcgttg
     6901 gaactccaag gaaaaccttc tcgctggacc cagtgaaaat gaccccaacc ttttcgttgc
     6961 actgtatgat tttgtggcca gtggagataa cactctaagc ataactaaag gtgaaaagct
     7021 ccgggtctta ggctataatc acaatgggga atggtgtgaa gcccaaacca aaaatggcca
     7081 aggctgggtc ccaagcaact acatcacgcc agtcaacagt ctggagaaac actcctggta
     7141 ccatgggcct gtgtcccgca atgccgctga gtatctgctg agcagcggga tcaatggcag
     7201 cttcttggtg cgtgagagtg agagcagtcc tggccagagg tccatctcgc tgagatacga
     7261 agggagggtg taccattaca ggatcaacac tgcttctgat ggcaagctct acgtctcctc
     7321 cgagagccgc ttcaacaccc tggccgagtt ggttcatcat cattcaacgg tggccgacgg
     7381 gctcatcacc acgctccatt atccagcccc aaagcgcaac aagcccactg tctatggtgt
     7441 gtcccccaac tacgacaagt gggagatgga acgcacggac atcaccatga agcacaagct
     7501 gggcgggggc cagtacgggg aggtgtacga gggcgtgtgg aagaaataca gcctgacggt
     7561 ggccgtgaag accttgaagg aggacaccat ggaggtggaa gagttcttga aagaagctgc
     7621 agtcatgaaa gagatcaaac accctaacct ggtgcagctc cttggggtct gcacccggga
     7681 gcccccgttc tatatcatca ctgagttcat gacctacggg aacctcctgg actacctgag
     7741 ggagtgcaac cggcaggagg tgaacgccgt ggtgctgctg tacatggcca ctcagatctc
     7801 gtcagccatg gagtacctgg agaagaaaaa cttcatccac agagatcttg ctgcccgaaa
     7861 ctgcctggta ggggagaacc acttggtgaa ggtagctgat tttggcctga gcaggttgat
     7921 gacaggggac acctacacag cccatgctgg agccaagttc cccatcaaat ggactgcacc
     7981 cgagagcctg gcctacaaca agttctccat caagtccgac gtctgggcat ttggagtatt
     8041 gctttgggaa attgctacct atggcatgtc cccttacccg ggaattgacc tgtcccaggt
     8101 gtatgagctg ctagagaagg actaccgcat ggagcgccca gaaggctgcc cagagaaggt
     8161 ctatgaactc atgcgagcat gttggcagtg gaatccctct gaccggccct cctttgctga
     8221 aatccaccaa gcctttgaaa caatgttcca ggaatccagt atctcagacg aagtggaaaa
     8281 ggagctgggg aaacaaggcg tccgtggggc tgtgagtacc ttgctgcagg ccccagagct
     8341 gcccaccaag acgaggacct ccaggagagc tgcagagcac agagacacca ctgacgtgcc
     8401 tgagatgcct cactccaagg gccagggaga gagcgatcct ctggaccatg agcctgccgt
     8461 gtctccattg ctccctcgaa aagagcgagg tcccccggag ggcggcctga atgaagatga
     8521 gcgccttctc cccaaagaca aaaagaccaa cttgttcagc gccttgatca agaagaagaa
     8581 gaagacagcc ccaacccctc ccaaacgcag cagctccttc cgggagatgg acggccagcc
     8641 ggagcgcaga ggggccggcg aggaagaggg ccgagacatc agcaacgggg cactggcttt
     8701 cacccccttg gacacagctg acccagccaa gtccccaaag cccagcaatg gggctggggt
     8761 ccccaatgga gccctccggg agtccggggg ctcaggcttc cggtctcccc acctgtggaa
     8821 gaagtccagc acgctgacca gcagccgcct agccaccggc gaggaggagg gcggtggcag
     8881 ctccagcaag cgcttcctgc gctcttgctc cgcctcctgc gttccccatg gggccaagga
     8941 cacggagtgg aggtcagtca cgctgcctcg ggacttgcag tccacgggaa gacagtttga
     9001 ctcgtccaca tttggagggc acaaaagtga gaagccggct ctgcctcgga agagggcagg
     9061 ggagaacagg tctgaccagg tgacccgagg cacagtaacg cctcccccca ggctggtgaa
     9121 aaagaatgag gaagctgctg atgaggtctt caaagacatc atggagtcca gcccgggctc
     9181 cagcccgccc aacctgactc caaaacccct ccggcggcag gtcaccgtgg cccctgcctc
     9241 gggcctcccc cacaaggaag aagctggaaa gggcagtgcc ttagggaccc ctgctgcagc
     9301 tgagccagtg acccccacca gcaaagcagg ctcaggtgca ccagggggca ccagcaaggg
     9361 ccccgccgag gagtccagag tgaggaggca caagcactcc tctgagtcgc cagggaggga
     9421 caaggggaaa ttgtccaggc tcaaacctgc cccgccgccc ccaccagcag cctctgcagg
     9481 gaaggctgga ggaaagccct cgcagagccc gagccaggag gcggccgggg aggcagtcct
     9541 gggcgcaaag acaaaagcca cgagtctggt tgatgctgtg aacagtgacg ctgccaagcc
     9601 cagccagccg ggagagggcc tcaaaaagcc cgtgctcccg gccactccaa agccacagtc
     9661 cgccaagccg tcggggaccc ccatcagccc agcccccgtt ccctccacgt tgccatcagc
     9721 atcctcggcc ctggcagggg accagccgtc ttccaccgcc ttcatccctc tcatatcaac
     9781 ccgagtgtct cttcggaaaa cccgccagcc tccagagcgg atcgccagcg gcgccatcac
     9841 caagggcgtg gtcctggaca gcaccgaggc gctgtgcctc gccatctcta ggaactccga
     9901 gcagatggcc agccacagcg cagtgctgga ggccggcaaa aacctctaca cgttctgcgt
     9961 gagctatgtg gattccatcc agcaaatgag gaacaagttt gccttccgag aggccatcaa
    10021 caaactggag aataatctcc gggagcttca gatctgcccg gcgacagcag gcagtggtcc
    10081 agcggccact caggacttca gcaagctcct cagttcggtg aaggaaatca gtgacatagt
    10141 gcagaggtag tctagaggat ccggctccgg cgagggcagg ggaagtctac taacatgcgg
    10201 ggacgtggag gaaaatcccg gcccagctag cgtgtgatca gaattcgttc cggagtcgtc
    10261 gactcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
    10321 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
    10381 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
    10441 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
    10501 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
    10561 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
    10621 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
    10681 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
    10741 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
    10801 cgcatcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
    10861 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
    10921 gccacttttt aaaagaaaag gggggactgg aagggctacg taactcccaa cgaagacaag
    10981 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11041 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    11101 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    11161 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
    11221 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
    11281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    11341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
    11401 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    11461 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    11521 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
    11581 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    11641 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    11701 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    11761 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11821 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    11881 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    11941 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    12001 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    12061 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    12121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    12181 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    12241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    12301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    12361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    12421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    12481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    12541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    12601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    12661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    12721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    12781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    12841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    12901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    12961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    13021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    13081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    13141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    13201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    13261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    13321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    13381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    13441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    13501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    13561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    13621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    13681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    13741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    13801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    13861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctca cttgagcgtc
    13921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    13981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    14041 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    14101 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    14161 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    14221 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    14281 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    14341 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    14401 gggaacaaaa gctggagctg ca
//
