LOCUS       sequence-415676-        9909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A control CE1 construct with catalytically attenuated EcKlenow DNA 
            polymerase, expressed from CMV or T7 promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: Control click editor -
            pCMV-T7-PCV2-nCas9-dead...
FEATURES             Location/Qualifiers
     source          1..9909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    101..122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(137..142,143..160,161..167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    175..191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     180..202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     199..215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     199..215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    286..510
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     499..516
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(539..556)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(565..585)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2470..2490)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2548..4131,4132..4134,4135..6648))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2548..6648)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7084..7103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7085..7103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7145..7348)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7174..7194)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7349..7728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7900..7919
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7998..8102
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8103..8171,8172..8963)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8321..8340)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9134..9722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9623..9642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9876..9893
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ctaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag
      241 aggtcgacgg tatcgagccc cagctggttc tttccgcctc agaagccata gagcccaccg
      301 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
      361 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
      421 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
      481 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaactcaat
      541 ggtgatggtg atgatgaccg gtcagacttt cctcttcttc ttgggagact cgaattcgct
      601 gccgtcggcg gttctttttg agccgccaga gtgggcctgg tcccaattct ctccgcttcc
      661 aacctcgacc agcagtggca cgtccagtct ggtgcaattt tccatcagct ggtgaatctg
      721 cttggccaca gcatccacgt cgtccttgtg cacctcaaac accagctccg cgtgcacctg
      781 catgatcatc cgcactctag gctgctcggc ctgcagccag gcgtccacgg cgatcatggc
      841 ccgcttgatg atgtcggcgg cggtgccctg cataggggcg ttgatagcag ctctctcagc
      901 ggcggctctt ctggcgccgt tgctgctctt gatgtcaggc agatacagtc tccggccatc
      961 cagggtttcc acgtatccct gctctttagc ctgggctctg gtccgttcca tgtactccag
     1021 cacgccgggg taccgctcga agtacaggtc catgtatttc tgggcctcct ttctagggat
     1081 gttgagctgt ctggccaggc caaaggcgct catgccgtag atcaggccga agttaatggc
     1141 cttggcggac cttctttgct cgctggtcac tgtttccaga ggcaggccga acacttcggc
     1201 agcggtggct ctgtggatgt ccttgccttc tgcaaaagcg gtcagcaggc ccttgtcccg
     1261 agacagatgg gccatgattc tcagttctat ctggctgtac gcagcagaca cgatgacgta
     1321 gtcctcaggg gcaatgaaag cctgtctgat tcttctgccc tcttcgttcc gcactgggat
     1381 gttttgcagg ttaggatctg tgctgctcag ccggccggtg gctgtcacgg cctggtgata
     1441 agatgtatgc acccggccgg tcttggggtt gatcatcaga ggcagcttgt cggtgtatgt
     1501 agacttcagc ttggccagtc ctctatattc caggatcact ttgggcagag ggtaatccag
     1561 cgccagttcc tccagcactt cttcgctggt gctgggggct ccgccaggtg tctttttcag
     1621 aggcttaatg ccctgcttct cgaacaggat tgtttgtagc tgcttggtgc tagacaggtt
     1681 gaactcctcg ccggcgattt cgtgggcctt cttttccagc tcggccagcc gcagtgtcag
     1741 ttcctcgctg tggttgtgca gcaccttggg gtcgatcttc acgccattcc tctcgattct
     1801 gctcaggacg ggcaccagag gcatctcgat gttctcgaac acgtttagtg ggcccttgtg
     1861 cttctgcaga tcgggccaca tcttcaggtg cagctgaagg gtcacgtcgg cgtcctcagc
     1921 ggcgtatctg ccggcttctt ccagtgcgat ctgattaaat gtcagctggt tcttgccctt
     1981 tccggcgatc tcttcgaagg tgatggtttt gtgcttcagc caccgttcag ccaggctgtc
     2041 catgtcgtgg cggccggcca cgctgttcag gatgtaagat tccagcatgg tatcgaaggc
     2101 gatgcctctc agctcgatgc cgtaattagc caggattccc ctatcgtatt tcaggttctg
     2161 tcccaccttc agggccttct cgtcctccag caggggcttc aggagctcca gtgctctctc
     2221 tctgctgatt tgatctgggg catccaggta atcgtgggcc acggggatgt aggcggccac
     2281 gccaggctcg atggcgaaag acaggcccac caggttggcg ctgatgttat ctaaggagtc
     2341 ggtggcggtg gcgaaggcga acacaggggc tttttccagt ttagcgatcc aagccttcag
     2401 tgtctcttcg tccaggatgg tcacgtagtt gtcgtaggag ataacgcttc cgccgctaga
     2461 gcctccgctc acctttcttt tcttcttagg gctctcgaat tcagagccgt cggcggtccg
     2521 cttgctgcca ccagagcttc cgccggagtc acctcccagc tgagacaggt cgatccgtgt
     2581 ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct
     2641 ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag
     2701 attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg
     2761 gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt
     2821 ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct gttccacaaa
     2881 cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt ggctggccag
     2941 gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct gcagttcgcc
     3001 ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt acttaggcag
     3061 cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa agtcgatggg
     3121 attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt
     3181 cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat aggccacggt
     3241 ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt
     3301 atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt
     3361 ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg caaaatcccg
     3421 gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag gccgcttccg
     3481 gatctcgccg ttggccaggg taatctcggt cttgaaaaag ttcatgatgt tgctgtagaa
     3541 gaagtacttg gcggtagcct tgccgatttc ctgctcgctc ttggcgatca tcttccgcac
     3601 gtcgtacacc ttgtagtcgc cgtacacgaa ctcgctttcc agcttagggt actttttgat
     3661 cagggcggtt cccacgacgg cgttcaggta ggcgtcgtgg gcgtggtggt agttgttgat
     3721 ctcgcgcact ttgtaaaact ggaaatcctt ccggaaatcg gacaccagct tggacttcag
     3781 ggtgatcact ttcacttccc ggatcagctt gtcattctcg tcgtacttag tgttcatccg
     3841 ggagtccagg atctgtgcca cgtgctttgt gatctgccgg gtttccacca gctgtctctt
     3901 gatgaagccg gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc
     3961 gaactttctc tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt
     4021 cttcacgacc tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt
     4081 cagcaccttg ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tagcgtccac
     4141 atcgtagtcg gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt
     4201 ctgcaggtag tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc
     4261 tttcaggatc tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg
     4321 gctgttcttc tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt
     4381 ctcgggcttg tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag
     4441 gatgcccttc ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc
     4501 gccctggccg gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg
     4561 gatcagctgc atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt
     4621 cttgccggac tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc
     4681 ggtgtatctc cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt
     4741 tttcagccgt tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc
     4801 ttccagaatg tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag
     4861 cagatcgtgg tatgtgccca gggaggcgtt gaaccgatct tccacgccgg agatttccac
     4921 ggagtcgaag cactcgattt tcttgaagta gtcctctttc agctgcttca cggtcacttt
     4981 ccggttggtc ttgaacagca ggtccacgat ggcctttttc tgctcgccgc tcaggaaggc
     5041 gggctttctc attccctcgg tcacgtattt cactttggtc agctcgttat acacggtgaa
     5101 gtactcgtac agcaggctgt gcttgggcag caccttctcg ttgggcaggt tcttatcgaa
     5161 gttggtcatc cgctcgatga agctctgggc ggaagcgccc ttgtccacca cttcctcgaa
     5221 gttccagggg gtgatggttt cctcgctctt tctggtcatc caggcgaatc tgctgtttcc
     5281 cctggccaga gggcccacgt agtaggggat gcggaaggtc aggatcttct cgatcttttc
     5341 ccggttgtcc ttcaggaatg ggtaaaaatc ttcctgccgc cgcagaatgg cgtgcagctc
     5401 tcccaggtgg atctggtggg ggatgctgcc gttgtcgaag gtccgctgct tccgcagcag
     5461 gtcctctctg ttcagcttca cgagcagttc ctcggtgccg tccatctttt ccaggatggg
     5521 cttgatgaac ttgtagaact cttcctggct ggctccgccg tcaatgtagc cggcgtagcc
     5581 gttcttgctc tggtcgaaga aaatctcttt gtacttctca ggcagctgct gccgcacgag
     5641 agctttcagc agggtcaggt cctggtggtg ctcgtcgtat ctcttgatca tagaggcgct
     5701 caggggggcc ttggtgatct cggtgttcac tctcaggatg tcgctcagca ggatggcgtc
     5761 ggacaggttc ttggcggcca gaaacaggtc ggcgtactgg tcgccgatct gggccagcag
     5821 gttgtccagg tcgtcgtcgt aggtgtcctt gctcagctgc agtttggcat cctcggccag
     5881 gtcgaagttg ctcttgaagt tgggggtcag gcccaggctc agggcaatca ggtttccgaa
     5941 caggccattc ttcttctcgc cgggcagctg ggcgatcaga ttttccagcc gtctgctctt
     6001 gctcagtctg gcagacagga tggccttggc gtccacgccg ctggcgttga tggggttttc
     6061 ctcgaacagc tggttgtagg tctgcaccag ctggatgaac agcttgtcca cgtcgctgtt
     6121 gtcggggttc aggtcgccct cgatcaggaa gtggccccgg aacttgatca tgtgggccag
     6181 ggccagatag atcagccgca ggtcggcctt gtcggtgctg tccaccagtt tctttctcag
     6241 gtggtagatg gtggggtact tctcgtggta ggccacctcg tccacgatgt tgccgaagat
     6301 ggggtgccgc tcgtgcttct tatcctcttc caccaggaag gactcttcca gtctgtggaa
     6361 gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg
     6421 gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt
     6481 ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt
     6541 gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca
     6601 gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcgc tgccgccgct
     6661 gctctcaggg gtggcggact cagatgtgcc gggtgtctcg gagccgctag agcctccgct
     6721 tctctgtccc tgggatctag gggcgccaca ttccatcagc agattgcctt ccttgctgca
     6781 gtactctttg ttctgctggt cggtgccctt ggccttctcg atgtggcatc tagcgcccag
     6841 gtaccacttc accttgttga aggtctgctt tttcacgaag ttggcgaagc cctgcaggtg
     6901 gggggtccgg ccttcctcat ttccttcctc gcccacgatg aagtagtcga acaggctgat
     6961 aggcaggtcc cggatcttct ttctctcatc ttcgctgggg ttgttcaggg taaagaccca
     7021 ccgcttgtga ggttgaggtc cgcttctgcc gtttttctta gatgggctca tggtggcggc
     7081 tctccctata gtgagtcgta ttagcggccg cggatctcta gcggatctga cggttcacta
     7141 aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     7201 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     7261 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     7321 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     7381 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     7441 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     7501 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     7561 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     7621 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     7681 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
     7741 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
     7801 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
     7861 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
     7921 tgagagtcga ccctagggga tcgggagatc gatctcccga tccgtcgacg tcaggtggca
     7981 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8041 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8101 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8161 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8221 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8281 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8341 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8401 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8461 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8521 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8581 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8641 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8701 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8761 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggtt
     8821 cgcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8881 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8941 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9001 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9061 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9901 aagagcgcc
//
