LOCUS       sequence-415677-       13302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double 
            HA tag in domain II and mutations in the P loop of Domains I, II, 
            III and IV which abolish trafficking to the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 13302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E13...
FEATURES             Location/Qualifiers
     source          1..13302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(534..552)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(535..552)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(572..589)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    578..802
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      848..1276
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(935..954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1145..1166
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1285..1305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1290..1619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1470..1605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1532..1551
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1686..2480
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1740..1759)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2350..2369
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2654..2775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2691..2710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2745..2764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2824..2840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2824..2840)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2837..2859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2848..2864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2872..2878,2879..2896,2897..2902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2917..2938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3055..3072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3226..3814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3306..3325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3985..4776,4777..4845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4608..4627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4846..4950)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5025..5044)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5216..5595
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5596..5799
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5750..5770
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5844..5863
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5844..5862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5954..6667
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5954..6667
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5984..6012)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6607..6627
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             8366..8392
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8396..8422
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cccccagacc cccttgaccc cccgccccag cgtcacctac aagacggcca actcctcgcc
       61 tgtccacttc gccggggcac cgagcggcct ccccgccttc tcccccggcc ggctcagccg
      121 gggcctctca gaacacaacg ccctgctgca gagggacccg ctcagccggc ccctggcgcc
      181 cggctcgcgc atcggctccg acccctacct ggggcagcgg ctggacagcg aggcgcccgc
      241 ccgtgccctg cccgaggacg cgcccgcctt tgaggagacc gcggccagca actcgggccg
      301 ctcctcccgg acctcgtacg tgtcctcact gacctcgcag cccccgccgc tgcgccgcgt
      361 gcccaacggc taccactgca ctctggggct gggcgggggc ggccgggcgc ggcgcggctg
      421 ccaccacccc gaccgcgacc gccggtgcta gactagttct agcaagcttc gacgaattct
      481 gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc tattctatag
      541 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
      601 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      661 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      721 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      781 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
      841 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      901 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      961 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1021 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1081 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1141 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1201 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1261 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     1321 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     1381 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1441 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1501 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     1561 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     1621 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     1681 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     1741 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     1801 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     1861 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     1921 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     1981 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     2041 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     2101 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     2161 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     2221 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     2281 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     2341 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     2401 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     2461 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     2521 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     2581 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     2641 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2701 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2761 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     2821 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2881 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2941 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3001 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3661 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3961 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4861 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4921 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     4981 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     5041 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     5101 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     5161 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     5221 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     5281 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     5341 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     5401 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     5461 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     5521 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     5581 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     5641 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     5701 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     5761 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     5821 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
     5881 cgagctcgga tccactagta acggccgcca gtgtgctgga attcctcgag ggtaccgagc
     5941 tcggatctaa aaaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
     6001 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     6061 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     6121 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     6181 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     6241 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     6301 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     6361 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     6421 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     6481 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     6541 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     6601 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     6661 atacaaaatg gtccgcttcg gggacgagct gggcggccgc tatgggggcg cgggcggcgc
     6721 ggagcgggcc cggggcggcg gggccggcgg ggctggcggc ccgggccccg gggggctgcc
     6781 gccgggccag cgggtcctct acaaacagtc gatcgcgcag cgcgcgcgga ccatggcgct
     6841 gtacaacccc atcccggtca agcagaactg cttcaccgtc aaccgctcgc tcttcgtctt
     6901 cagcgaggac aacgtcgtcc gcaaatacgc caagcgcatc accgagtggc ctccgttcga
     6961 gtacatgatc ctggccacca tcatcgccaa ctgcatcgtg ctggccctgg agcagcacct
     7021 ccctgacggg gacaagacgc ctatgtccga gcgcctggat gacaccgagc cctacttcat
     7081 tgggatcttc tgcttcgagg cggggatcaa gatcctggcc ctgggctttg ttctgcacaa
     7141 gggctcctat ctgcggaacg gctggaacgt catggacttc gtggtagtcc tcacggggat
     7201 tcttgccaca gccggaaccg acttcgacct gcgaacgctg agggctgtgc gtgtgctaag
     7261 gcccctgaag ctggtgtctg ggattccgag cttgcaagtg gtgctcaagt ccatcatgaa
     7321 ggccatggtc ccgctgctgc agatcgggct gctgctcttc ttcgccatcc tcatgtttgc
     7381 catcatcggc ctcgagttct acatgggcaa gttccacaag gcctgcttcc ccaacagcac
     7441 agacccagat cccgtgggtg acttcccctg tggcaaggag gccccggccc ggctctgtga
     7501 gggagacacg gagtgccggg agtactgggc cggacccaac tttggtatca ccaactttga
     7561 caacatcctg tttgccatct tgaccgtgtt ccagtgcatc accatggcgg gctggactga
     7621 catcctctac aatacaaacg atgcagccgg caacacgtgg aactggctct acttcatccc
     7681 tctcatcatc atcggctcct tcttcatgct caacctggtg ctgggtgtgc tctcaggaga
     7741 gtttgctaag gagcgcgaga gagtggagaa ccgccgcgcc ttcctgaagc tccggcggca
     7801 gcagcagatt gagcgggagc tcaacgggta cctggagtgg atcttcaagg ctgaggaagt
     7861 catgctggct gaggaggaca ggaacgcaga ggagaagtcc cctctggatg cagtgttgaa
     7921 gagagcagct gccaagaaga gcagaagcga cctgatccag gccgaggagg gggagggccg
     7981 gctcacaggc ctctgtgctc ccggttcccc gttcgcccgt gccagcctga aaagcgggaa
     8041 gacggagagc tcgtcgtact tccggaggaa ggagaagatg ttccgcttct tcatccggcg
     8101 catggtgaag gcacagagct tctactggac ggtgctgtgt gtggtggcac tgaacacact
     8161 gtgcgtggcc atggtgcact acaaccagcc gcagcggctc accacggcat tgtactttgc
     8221 agagtttgtt ttcctgggtc tcttcctcac agagatgtcc ctgaagatgt atggcctggg
     8281 gcccaggagc tacttccggt cctccttcaa ctgttttgac tttggggtca tcgtgggcag
     8341 tatctttgaa gtggtctggg ctgcttaccc ctacgacgtc cccgactacg ccggctaccc
     8401 gtacgacgtc ccggactacg ccggcgtcga cgtcaagcca gggacctcct tcgggatcag
     8461 tgttctgcgg gctctgcgct tactgagaat cttcaaggtc accaaatact ggaactccct
     8521 acggaacctg gtggtgtctc tgctcaactc catgaagtcc atcatcagcc tcctcttcct
     8581 gctcttcctg ttcattgttg tctttgctct gctggggatg cagctgtttg gaggccagtt
     8641 caactttaaa gacgagaccc caacgaccaa ctttgacacc ttccctgctg ctatcctcac
     8701 cgtcttccag atcctgaccg gagcggactg gaatgcggtc atgtaccacg ggatcgagtc
     8761 gcaaggtggc gtcagcagag gaatgttctc ctccttctac ttcattgtcc tcactctgtt
     8821 tgggaactac accctgctga atgtcttcct ggccattgct gtggacaacc ttgccaatgc
     8881 tcaggagctg accaaggatg aagaggagat ggaagaagca gccaatcaga aactcgcctt
     8941 gcagaaggcc aaggaagtgg ctgaagtcag ccccatgtct gctgcgaaca tctccattgc
     9001 cgccaggcag cagaactcgg ccaaagcgcg ctcggtgtgg gagcagcgag ccagccagct
     9061 gcggctgcag aacctgaggg ccagctgcga ggcgctgtac agcgagatgg accccgagga
     9121 gaggctgcgc tacgccaccg cgcgccacct gcggcccgac gtgaagacgc acctggaccg
     9181 gccgctggtg gttgagcccg gccgcgacgc tccgcggggc cccccggggg ggaagtcccg
     9241 gccggatggc tcggaggccc ccgagggtgc ggatcccccg cgcaggcatc accggcaccg
     9301 agacaaggac aaggctcccg ccacggtccc ctcggcgggg gaacaggaca gagccgaagc
     9361 tctgagggca gagggcgggg agctggggcc ccgcgaggag cgggggcggc cacgccgcag
     9421 ccgcagcaag gaggccccgg gcgccccgga ggtgcgcagc gatcgcggcc gtggcccgtg
     9481 tcctgaaggt ggtcggcgcc accaccggcg cgggtcaccg gaggaggcag ctgagaggga
     9541 gccccgccgc caccgcgcgc accggcacgg gcccgacccg ggcaaggagg gcccagcgtc
     9601 cgggaccagg ggcgagcggc gcgcacgaca ccgcacgggc ccccgggcgt gcccccggga
     9661 ggctgagagc agcgaggagc ctgcgcggcg gcaccgggct cggcacaagg cgccgcccac
     9721 ccaggagacc gcggagaagg acaaggaggc tgccgagaag gggggcgagg ccacggaggc
     9781 cgagaaggac aaggaggctc ggaaccacca gcccaaggag ctgccctgtg acctggaggc
     9841 catcggcatg ctgggagtgg gcgctgtgca tacgctgccc agcacctgtc tgcagaaggt
     9901 ggaggagcag ccagaggatg cggacaacca gcggaacgtc acccgcatgg gtagtcagcc
     9961 cccggacacc agcaccactg tgcacattcc tgtgacgctg accggccccc ccggggagac
    10021 cacggtagtg cccagtggaa acgtggacct ggaaagtcaa gcagagggga agaaggaggt
    10081 ggaaaccagc gatgtgatga ggagcggccc tcggcccatt gtcccgtaca gttccatgtt
    10141 ctgtttaagc ccaaccaacc tgctgcgccg ctgctgccac tacattgtga ccatgcgata
    10201 cttcgagatg gtcatcctcg tggtcatcgc cctgagcagc atcgccctgg ctgccgagga
    10261 ccccgtgaga acagactctc ccaggaataa tgctctgaag tacatggact acatcttcac
    10321 aggcgtcttc accttcgaga tggtgataaa gatgatcgat ttgggactgc tgctacaccc
    10381 tggagcctat ttccgggatc tgtggaacat tctggacttc attgtggtca gtggggccct
    10441 ggtggcattt gctttctcag gatccaaagg gaaagacatc agcaccatca aatccctgag
    10501 agtcctgcgt gtgctgcggc cactcaagac catcaagcgg ctgcccaagc tcaaggccgt
    10561 gtttgactgt gtggtgaact cactgaaaaa cgtcctcaac atcctgatcg tctacatgct
    10621 cttcatgttc atctttgccg tcattgccgt gcagctcttc aaagggaagt tcttctactg
    10681 cacggacgaa tccaaggagc tggagcgtga ctgcaggggt cagtatttgg attatgagaa
    10741 ggaggaagtg gaagctcaac cgaggcagtg gaagaaatat gacttccact atgacaacgt
    10801 gctctgggct ctgctaacgc tcttcacggt gtccacaggg gcagggtggc ccatggtgct
    10861 gaaacactct gtggatgcca cctacgagga gcagggcccg agccccgggt accgcatgga
    10921 gctctccatc ttctacgtgg tctactttgt ggtctttccc ttcttctttg tcaacatctt
    10981 cgtggccttg atcatcatca ccttccagga gcagggggac aaggtgatgt ccgagtgcag
    11041 cctggagaag aacgagaggg cttgcatcga ctttgccatc agcgccagac ccctgacgcg
    11101 gtacatgccc cagaacaagc agtcgttcca gtataagacg tggacgttcg tggtgtcccc
    11161 accttttgag tacttcatca tggctatgat cgccctcaac actgtggtgc tgatgatgaa
    11221 gttctacgat gcgccgtacg agtatgagct aatgctgaaa tgcctgaaca tcgtgttcac
    11281 gtccatgttc tccatggagt gtgtgctgaa gatcatcgcc ttcggggtgc tgaactattt
    11341 cagagatgcc tggaatgtct ttgactttgt cacggtgttg ggaagtatta ctgatatttt
    11401 ggtaacagag attgcgaaca acttcatcaa cctcagcttc ctgcgcctgt tccgggccgc
    11461 gcggctcatc aagctgctgc gccagggcta caccatccgc atcctgcttt ggaccttcgt
    11521 gcagtccttc aaggcgctgc cctacgtgtg tctcctcatc gccatgctct tcttcatcta
    11581 cgccatcatt ggcatgcagg tttttgggaa catcgccctg gacgatgaca ccagcatcaa
    11641 caggcacaat aacttccgga cgtttctgca ggccttgatg ttgctgttca ggagcgccac
    11701 gggggcggcc tggcacgaga taatgctgtc gtgcctcagc agccgggcct gcgacgagca
    11761 ctccaatgcc agcgagtgcg gcagcgactt tgcctacttt tacttcgtct ccttcatctt
    11821 cctctgctcc tttctgatgt tgaacctctt tgtggctgtg atcatggaca attttgagta
    11881 ccttacacgg gactcttcca tcttagggcc tcaccacctg gatgagttca tccgggtctg
    11941 ggctgaatac gacccggccg cgtgtgggcg catcagttac agtgacatgt ttgagatgct
    12001 gaagcacatg tccccacccc tggggctggg gaagaagtgc cctgctcgag tcgcgtacaa
    12061 gcgcctggtc cgcatgaaca tgcccatctc cagtgaggac atgacggtgc acttcacatc
    12121 cacgctgatg gctctcattc ggacagcgct ggacatcaag ctggcaccag ctgggaccaa
    12181 gcagcaccag tgcgacgcag agctgaggaa ggagatttcc tgtgtgtggg ccaatctgcc
    12241 ccagaagacc ctggacttac tggtaccacc ccataagcct gacgagatga cggtgggcaa
    12301 agtctatgca gctctgatga tcttcgactt ctacaaacag aacaaaacca gcagagacca
    12361 gactcagcaa gcccccggag gcctgtccca gctgggcccc gtgtccctgt tccacccgct
    12421 gaaggccacg ctggagcaga ctcagccggc gctgcgtgga gcccgggctt tcctgcggca
    12481 gaagagttct gcctccctga gcaacggcgg ggccgtacaa acccaggaga gcggcatcaa
    12541 ggaatcggtt tcttggggca ctcagaggac ccaggatgtg ctttgcgagg ccagggcacc
    12601 cctggagcgg ggccactcag ctgagatccc agtcgggcag cctgggacac tggctgtaga
    12661 tgtccagatg cagaacatga ctctgagtgg ccctgacgcc gagccgcagc cagggctgga
    12721 gagccagggc cgagccgcct ccatgccccg cctggccgca gaaacacagc ccgcgcctga
    12781 cgccagcccc atgaagcgct ccatctccac gctggccccg cgcccccaca cggcccggct
    12841 cggcagcacc gcgctggacc gcccagcccc cagccaggcc ccgcaccatc accaccaccg
    12901 ctgccaccgc cgcagggaca ggaagcagcg gtccctggag aagggtccca gcctgtctgc
    12961 tgacacagat ggggcccccg acagcacggt ggggccagga ctgcccacgg gggagggccc
    13021 ccccggctgc aggcgggagc gggagcgcag gcaggagcgg ggccggtccc aggagcggag
    13081 gcagccctcg tcctcctcct cggagaagca tcgcttctac tcctgtgacc gcttcggggg
    13141 ccgcgaaccc ccacagccca agccctccct cagtagtcac cccacgtcgc cgacggccgg
    13201 gcaggagccg ggaccccacc cgcaggggag tggttcggtt cacgggagcc ccctgctgtc
    13261 cacatctggt gctagcaccc cgggccgggg gcggaggcag ct
//
