LOCUS       sequence-415686-        9909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A variant CE1 construct with MSMV HUH endonuclease, expressed from 
            CMV or T7 promoters. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: HUHe variant click editor (CE1) -
            pCMV-T7-MSMV...
FEATURES             Location/Qualifiers
     source          1..9909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(107..126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      920..1508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1409..1428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1662..1679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1796..1817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1832..1837,1838..1855,1856..1862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1870..1886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1875..1897
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1894..1910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1894..1910
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1981..2205
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2194..2211
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2234..2251)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2260..2280)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4165..4185)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(4243..5826,5827..5829,5830..8343))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4243..8343)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(8779..8798)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8780..8798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8840..9043)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8869..8889)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9044..9423
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9595..9614
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9693..9797
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9798..9866,9867..9909,1..749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtggttcgcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1741 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1801 tgagttagct cactcattag gcaccctagg ctttacactt tatgcttccg gctcgtatgt
     1861 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     1921 ccaagctcta gctagaggtc gacggtatcg agccccagct ggttctttcc gcctcagaag
     1981 ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     2041 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     2101 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     2161 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc
     2221 gggtttaaac tcaatggtga tggtgatgat gaccggtcag actttcctct tcttcttggg
     2281 agactcgaat tcgctgccgt cggcggttct ttttgagccg ccagagtggg cctggtccca
     2341 attctctccg cttccaacct cgaccagcag tggcacgtcc agtctggtgc aattttccat
     2401 cagctggtga atctgcttgg ccacagcatc cacgtcgtcc ttgtgcacct caaacaccag
     2461 ctcgtcgtgc acctgcatga tcatccgcac tctaggctgc tcggcctgca gccaggcgtc
     2521 cacggcgatc atggcccgct tgatgatgtc ggcggcggtg ccctgcatag gggcgttgat
     2581 agcagctctc tcagcggcgg ctcttctggc gccgttgctg ctcttgatgt caggcagata
     2641 cagtctccgg ccatccaggg tttccacgta tccctgctct ttagcctggg ctctggtccg
     2701 ttccatgtac tccagcacgc cggggtaccg ctcgaagtac aggtccatgt atttctgggc
     2761 ctcctttcta gggatgttga gctgtctggc caggccaaag gcgctcatgc cgtagatcag
     2821 gccgaagtta atggccttgg cggaccttct ttgctcgctg gtcactgttt ccagaggcag
     2881 gccgaacact tcggcagcgg tggctctgtg gatgtccttg ccttctgcaa aagcggtcag
     2941 caggcccttg tcccgagaca gatgggccat gattctcagt tctatctggc tgtagtcagc
     3001 agacacgatg acgtagtcct caggggcaat gaaagcctgt ctgattcttc tgccctcttc
     3061 gttccgcact gggatgtttt gcaggttagg atctgtgctg ctcagccggc cggtggctgt
     3121 cacggcctgg tgataagatg tatgcacccg gccggtcttg gggttgatca tcagaggcag
     3181 cttgtcggtg tatgtagact tcagcttggc cagtcctcta tattccagga tcactttggg
     3241 cagagggtaa tccagcgcca gttcctccag cacttcttcg ctggtgctgg gggctccgcc
     3301 aggtgtcttt ttcagaggct taatgccctg cttctcgaac aggattgttt gtagctgctt
     3361 ggtgctagac aggttgaact cctcgccggc gatttcgtgg gccttctttt ccagctcggc
     3421 cagccgcagt gtcagttcct cgctgtggtt gtgcagcacc ttggggtcga tcttcacgcc
     3481 attcctctcg attctgctca ggacgggcac cagaggcatc tcgatgttct cgaacacgtt
     3541 tagtgggccc ttgtgcttct gcagatcggg ccacatcttc aggtgcagct gaagggtcac
     3601 gtcggcgtcc tcagcggcgt atctgccggc ttcttccagt gcgatctgat taaatgtcag
     3661 ctggttcttg ccctttccgg cgatctcttc gaaggtgatg gttttgtgct tcagccaccg
     3721 ttcagccagg ctgtccatgt cgtggcggcc ggccacgctg ttcaggatgt aagattccag
     3781 catggtatcg aaggcgatgc ctctcagctc gatgccgtaa ttagccagga ttcccctatc
     3841 gtatttcagg ttctgtccca ccttcagggc cttctcgtcc tccagcaggg gcttcaggag
     3901 ctccagtgct ctctctctgc tgatttgatc tggggcatcc aggtaatcgt gggccacggg
     3961 gatgtaggcg gccacgccag gctcgatggc gaaagacagg cccaccaggt tggcgctgat
     4021 gttatctaag gagtcggtgg cggtggcgaa ggcgaacaca ggggcttttt ccagtttagc
     4081 gatccaagcc ttcagtgtct cttcgtccag gatggtcacg tagttgtcgt aggagataac
     4141 gcttccgccg ctagagcctc cgctcacctt tcttttcttc ttagggctct cgaattcaga
     4201 gccgtcggcg gtccgcttgc tgccaccaga gcttccgccg gagtcacctc ccagctgaga
     4261 caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg tggcgtccag
     4321 cacctctttg gtgctggtgt acctcttccg gtcgatggtg gtgtcaaagt acttgaaggc
     4381 ggcaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg cctgctctct
     4441 gatgggctta tcccggtgct tgttgtaggc ggacagcact ttgtccagat tagcgtcggc
     4501 caggatcact ctcttggaga actcgctgat ctgctcgatg atctcgtcca ggtagtgctt
     4561 gtgctgttcc acaaacagct gtttctgctc attatcctcg ggggagccct tcagcttctc
     4621 atagtggctg gccaggtaca ggaagttcac atatttggag ggcagggcca gttcgtttcc
     4681 cttctgcagt tcgccggcag aggccagcat tctcttccgg ccgttttcca gctcgaacag
     4741 ggagtactta ggcagcttga tgatcaggtc ctttttcact tctttgtagc ccttggcttc
     4801 cagaaagtcg atgggattct tctcgaagct gcttctttcc atgatggtga tccccagcag
     4861 ctctttcaca ctcttcagtt tcttggactt gcccttttcc actttggcca ccaccagcac
     4921 agaataggcc acggtggggc tgtcgaagcc gccgtacttc ttagggtccc agtccttctt
     4981 tctggcgatc agcttatcgc tgttcctctt gggcaggata gactctttgc tgaagccgcc
     5041 tgtctgcacc tcggtctttt tcacgatatt cacttggggc atgctcagca ctttccgcac
     5101 ggtggcaaaa tcccggccct tatcccacac gatctccccg gtttcgccgt ttgtctcgat
     5161 cagaggccgc ttccggatct cgccgttggc cagggtaatc tcggtcttga aaaagttcat
     5221 gatgttgctg tagaagaagt acttggcggt agccttgccg atttcctgct cgctcttggc
     5281 gatcatcttc cgcacgtcgt acaccttgta gtcgccgtac acgaactcgc tttccagctt
     5341 agggtacttt ttgatcaggg cggttcccac gacggcgttc aggtaggcgt cgtgggcgtg
     5401 gtggtagttg ttgatctcgc gcactttgta aaactggaaa tccttccgga aatcggacac
     5461 cagcttggac ttcagggtga tcactttcac ttcccggatc agcttgtcat tctcgtcgta
     5521 cttagtgttc atccgggagt ccaggatctg tgccacgtgc tttgtgatct gccgggtttc
     5581 caccagctgt ctcttgatga agccggcctt atccagttcg ctcaggccgc ctctctcggc
     5641 cttggtcaga ttgtcgaact ttctctgggt aatcagcttg gcgttcagca gctgccgcca
     5701 gtagttcttc atcttcttca cgacctcttc ggagggcacg ttgtcgctct tgccccggtt
     5761 cttgtcgctt ctggtcagca ccttgttgtc gatggagtcg tccttcagaa agctctgagg
     5821 cacgatagcg tccacatcgt agtcggacag ccggttgatg tccagttcct ggtccacgta
     5881 catatcccgc ccattctgca ggtagtacag gtacagcttc tcgttctgca gctgggtgtt
     5941 ttccacgggg tgttctttca ggatctggct gcccagctct ttgatgccct cttcgatccg
     6001 cttcattctc tcgcggctgt tcttctgtcc cttctgggtg gtctggttct ctctggccat
     6061 ttcgatcacg atgttctcgg gcttgtgccg gcccatcact ttcacgagct cgtccaccac
     6121 cttcactgtc tgcaggatgc ccttcttaat ggcggggctg ccggccagat tggcaatgtg
     6181 ctcgtgcagg ctatcgccct ggccggacac ctgggctttc tggatgtcct ctttaaaggt
     6241 caggctgtcg tcgtggatca gctgcatgaa gtttctgttg gcgaagccgt cggacttcag
     6301 gaaatccagg attgtcttgc cggactgctt gtcccggatg ccgttgatca gcttccggct
     6361 cagcctgccc cagccggtgt atctccgccg cttcagctgc ttcatcactt tgtcgtcgaa
     6421 caggtgggca taggttttca gccgttcctc gatcatctct ctgtcctcaa acagtgtcag
     6481 ggtcagcacg atatcttcca gaatgtcctc gttttcctca ttgtccagga agtccttgtc
     6541 cttgataatt ttcagcagat cgtggtatgt gcccagggag gcgttgaacc gatcttccac
     6601 gccggagatt tccacggagt cgaagcactc gattttcttg aagtagtcct ctttcagctg
     6661 cttcacggtc actttccggt tggtcttgaa cagcaggtcc acgatggcct ttttctgctc
     6721 gccgctcagg aaggcgggct ttctcattcc ctcggtcacg tatttcactt tggtcagctc
     6781 gttatacacg gtgaagtact cgtacagcag gctgtgcttg ggcagcacct tctcgttggg
     6841 caggttctta tcgaagttgg tcatccgctc gatgaagctc tgggcggaag cgcccttgtc
     6901 caccacttcc tcgaagttcc agggggtgat ggtttcctcg ctctttctgg tcatccaggc
     6961 gaatctgctg tttcccctgg ccagagggcc cacgtagtag gggatgcgga aggtcaggat
     7021 cttctcgatc ttttcccggt tgtccttcag gaatgggtaa aaatcttcct gccgccgcag
     7081 aatggcgtgc agctctccca ggtggatctg gtgggggatg ctgccgttgt cgaaggtccg
     7141 ctgcttccgc agcaggtcct ctctgttcag cttcacgagc agttcctcgg tgccgtccat
     7201 cttttccagg atgggcttga tgaacttgta gaactcttcc tggctggctc cgccgtcaat
     7261 gtagccggcg tagccgttct tgctctggtc gaagaaaatc tctttgtact tctcaggcag
     7321 ctgctgccgc acgagagctt tcagcagggt caggtcctgg tggtgctcgt cgtatctctt
     7381 gatcatagag gcgctcaggg gggccttggt gatctcggtg ttcactctca ggatgtcgct
     7441 cagcaggatg gcgtcggaca ggttcttggc ggccagaaac aggtcggcgt actggtcgcc
     7501 gatctgggcc agcaggttgt ccaggtcgtc gtcgtaggtg tccttgctca gctgcagttt
     7561 ggcatcctcg gccaggtcga agttgctctt gaagttgggg gtcaggccca ggctcagggc
     7621 aatcaggttt ccgaacaggc cattcttctt ctcgccgggc agctgggcga tcagattttc
     7681 cagccgtctg ctcttgctca gtctggcaga caggatggcc ttggcgtcca cgccgctggc
     7741 gttgatgggg ttttcctcga acagctggtt gtaggtctgc accagctgga tgaacagctt
     7801 gtccacgtcg ctgttgtcgg ggttcaggtc gccctcgatc aggaagtggc cccggaactt
     7861 gatcatgtgg gccagggcca gatagatcag ccgcaggtcg gccttgtcgg tgctgtccac
     7921 cagtttcttt ctcaggtggt agatggtggg gtacttctcg tggtaggcca cctcgtccac
     7981 gatgttgccg aagatggggt gccgctcgtg cttcttatcc tcttccacca ggaaggactc
     8041 ttccagtctg tggaagaagc tgtcgtccac cttggccatc tcgttgctga agatctcttg
     8101 cagatagcag atccggttct tccgtctggt gtatcttctt ctggcggttc tcttcagccg
     8161 ggtggcctcg gctgtttcgc cgctgtcgaa cagcagggct ccgatcaggt tcttcttgat
     8221 gctgtgccgg tcggtgttgc ccagcacctt gaatttcttg ctgggcacct tgtactcgtc
     8281 ggtgatcacg gcccagccca cagagttggt gccgatgtcc aggccgatgc tgtacttctt
     8341 gtcgctgccg ccgctgctct caggggtggc ggactcagat gtgccgggtg tctcggagcc
     8401 gctagagcct ccgctggcgc ccttggacac ctcgttgatg gtattctttc tgatgtaggc
     8461 caggcagtcc cgcacgtttc tacaagcctg gatgttgggg tggaacacca cggctccagg
     8521 gatgcttgtg ccggtgtaat cgaagtatgt tggatcaaag gtgctgattt cgattctggt
     8581 ctgaatcagc acatgagagt gcaggccgcc gtccttgtgg ttctcctggg ccacgtgcac
     8641 gtacagaggg tcgtaagggg tcacgaggtt ccacaggtgg tcgcacagga attcagggcc
     8701 gatggggcat ctaggatatg tcagaaagac atttttggcc cggaagcgga agctggtgtg
     8761 gctcatggtg gcggctctcc ctatagtgag tcgtattagc ggccgcggat ctctagcgga
     8821 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     8881 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     8941 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     9001 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     9061 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     9121 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     9181 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     9241 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     9301 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     9361 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
     9421 gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca
     9481 gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc
     9541 actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat
     9601 cagagcagat tgtactgaga gtcgacccta ggggatcggg agatcgatct cccgatccgt
     9661 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9781 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9841 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9901 aagatcagt
//
