LOCUS       sequence-415692-       10548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A variant CE1 construct with TraI HUH endonuclease, expressed from 
            CMV or T7 promoters. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 10548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: HUHe variant click editor (CE1) -
            pCMV-T7-TraI...
FEATURES             Location/Qualifiers
     source          1..10548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1062..1286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1275..1292
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1315..1332)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1341..1361)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3246..3266)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3324..4907,4908..4910,4911..7424))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3324..7424)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(8499..8518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8500..8518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8560..8763)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8589..8609)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8764..9143
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9315..9334
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9413..9517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9518..9586,9587..10378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9736..9755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
     1081 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1141 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     1201 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1261 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg
     1321 atggtgatga tgaccggtca gactttcctc ttcttcttgg gagactcgaa ttcgctgccg
     1381 tcggcggttc tttttgagcc gccagagtgg gcctggtccc aattctctcc gcttccaacc
     1441 tcgaccagca gtggcacgtc cagtctggtg caattttcca tcagctggtg aatctgcttg
     1501 gccacagcat ccacgtcgtc cttgtgcacc tcaaacacca gctcgtcgtg cacctgcatg
     1561 atcatccgca ctctaggctg ctcggcctgc agccaggcgt ccacggcgat catggcccgc
     1621 ttgatgatgt cggcggcggt gccctgcata ggggcgttga tagcagctct ctcagcggcg
     1681 gctcttctgg cgccgttgct gctcttgatg tcaggcagat acagtctccg gccatccagg
     1741 gtttccacgt atccctgctc tttagcctgg gctctggtcc gttccatgta ctccagcacg
     1801 ccggggtacc gctcgaagta caggtccatg tatttctggg cctcctttct agggatgttg
     1861 agctgtctgg ccaggccaaa ggcgctcatg ccgtagatca ggccgaagtt aatggccttg
     1921 gcggaccttc tttgctcgct ggtcactgtt tccagaggca ggccgaacac ttcggcagcg
     1981 gtggctctgt ggatgtcctt gccttctgca aaagcggtca gcaggccctt gtcccgagac
     2041 agatgggcca tgattctcag ttctatctgg ctgtagtcag cagacacgat gacgtagtcc
     2101 tcaggggcaa tgaaagcctg tctgattctt ctgccctctt cgttccgcac tgggatgttt
     2161 tgcaggttag gatctgtgct gctcagccgg ccggtggctg tcacggcctg gtgataagat
     2221 gtatgcaccc ggccggtctt ggggttgatc atcagaggca gcttgtcggt gtatgtagac
     2281 ttcagcttgg ccagtcctct atattccagg atcactttgg gcagagggta atccagcgcc
     2341 agttcctcca gcacttcttc gctggtgctg ggggctccgc caggtgtctt tttcagaggc
     2401 ttaatgccct gcttctcgaa caggattgtt tgtagctgct tggtgctaga caggttgaac
     2461 tcctcgccgg cgatttcgtg ggccttcttt tccagctcgg ccagccgcag tgtcagttcc
     2521 tcgctgtggt tgtgcagcac cttggggtcg atcttcacgc cattcctctc gattctgctc
     2581 aggacgggca ccagaggcat ctcgatgttc tcgaacacgt ttagtgggcc cttgtgcttc
     2641 tgcagatcgg gccacatctt caggtgcagc tgaagggtca cgtcggcgtc ctcagcggcg
     2701 tatctgccgg cttcttccag tgcgatctga ttaaatgtca gctggttctt gccctttccg
     2761 gcgatctctt cgaaggtgat ggttttgtgc ttcagccacc gttcagccag gctgtccatg
     2821 tcgtggcggc cggccacgct gttcaggatg taagattcca gcatggtatc gaaggcgatg
     2881 cctctcagct cgatgccgta attagccagg attcccctat cgtatttcag gttctgtccc
     2941 accttcaggg ccttctcgtc ctccagcagg ggcttcagga gctccagtgc tctctctctg
     3001 ctgatttgat ctggggcatc caggtaatcg tgggccacgg ggatgtaggc ggccacgcca
     3061 ggctcgatgg cgaaagacag gcccaccagg ttggcgctga tgttatctaa ggagtcggtg
     3121 gcggtggcga aggcgaacac aggggctttt tccagtttag cgatccaagc cttcagtgtc
     3181 tcttcgtcca ggatggtcac gtagttgtcg taggagataa cgcttccgcc gctagagcct
     3241 ccgctcacct ttcttttctt cttagggctc tcgaattcag agccgtcggc ggtccgcttg
     3301 ctgccaccag agcttccgcc ggagtcacct cccagctgag acaggtcgat ccgtgtctcg
     3361 tacaggccgg tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg
     3421 tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg
     3481 gtcagggtaa acaggtggat gatattctcg gcctgctctc tgatgggctt atcccggtgc
     3541 ttgttgtagg cggacagcac tttgtccaga ttagcgtcgg ccaggatcac tctcttggag
     3601 aactcgctga tctgctcgat gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc
     3661 tgtttctgct cattatcctc gggggagccc ttcagcttct catagtggct ggccaggtac
     3721 aggaagttca catatttgga gggcagggcc agttcgtttc ccttctgcag ttcgccggca
     3781 gaggccagca ttctcttccg gccgttttcc agctcgaaca gggagtactt aggcagcttg
     3841 atgatcaggt cctttttcac ttctttgtag cccttggctt ccagaaagtc gatgggattc
     3901 ttctcgaagc tgcttctttc catgatggtg atccccagca gctctttcac actcttcagt
     3961 ttcttggact tgcccttttc cactttggcc accaccagca cagaataggc cacggtgggg
     4021 ctgtcgaagc cgccgtactt cttagggtcc cagtccttct ttctggcgat cagcttatcg
     4081 ctgttcctct tgggcaggat agactctttg ctgaagccgc ctgtctgcac ctcggtcttt
     4141 ttcacgatat tcacttgggg catgctcagc actttccgca cggtggcaaa atcccggccc
     4201 ttatcccaca cgatctcccc ggtttcgccg tttgtctcga tcagaggccg cttccggatc
     4261 tcgccgttgg ccagggtaat ctcggtcttg aaaaagttca tgatgttgct gtagaagaag
     4321 tacttggcgg tagccttgcc gatttcctgc tcgctcttgg cgatcatctt ccgcacgtcg
     4381 tacaccttgt agtcgccgta cacgaactcg ctttccagct tagggtactt tttgatcagg
     4441 gcggttccca cgacggcgtt caggtaggcg tcgtgggcgt ggtggtagtt gttgatctcg
     4501 cgcactttgt aaaactggaa atccttccgg aaatcggaca ccagcttgga cttcagggtg
     4561 atcactttca cttcccggat cagcttgtca ttctcgtcgt acttagtgtt catccgggag
     4621 tccaggatct gtgccacgtg ctttgtgatc tgccgggttt ccaccagctg tctcttgatg
     4681 aagccggcct tatccagttc gctcaggccg cctctctcgg ccttggtcag attgtcgaac
     4741 tttctctggg taatcagctt ggcgttcagc agctgccgcc agtagttctt catcttcttc
     4801 acgacctctt cggagggcac gttgtcgctc ttgccccggt tcttgtcgct tctggtcagc
     4861 accttgttgt cgatggagtc gtccttcaga aagctctgag gcacgatagc gtccacatcg
     4921 tagtcggaca gccggttgat gtccagttcc tggtccacgt acatatcccg cccattctgc
     4981 aggtagtaca ggtacagctt ctcgttctgc agctgggtgt tttccacggg gtgttctttc
     5041 aggatctggc tgcccagctc tttgatgccc tcttcgatcc gcttcattct ctcgcggctg
     5101 ttcttctgtc ccttctgggt ggtctggttc tctctggcca tttcgatcac gatgttctcg
     5161 ggcttgtgcc ggcccatcac tttcacgagc tcgtccacca ccttcactgt ctgcaggatg
     5221 cccttcttaa tggcggggct gccggccaga ttggcaatgt gctcgtgcag gctatcgccc
     5281 tggccggaca cctgggcttt ctggatgtcc tctttaaagg tcaggctgtc gtcgtggatc
     5341 agctgcatga agtttctgtt ggcgaagccg tcggacttca ggaaatccag gattgtcttg
     5401 ccggactgct tgtcccggat gccgttgatc agcttccggc tcagcctgcc ccagccggtg
     5461 tatctccgcc gcttcagctg cttcatcact ttgtcgtcga acaggtgggc ataggttttc
     5521 agccgttcct cgatcatctc tctgtcctca aacagtgtca gggtcagcac gatatcttcc
     5581 agaatgtcct cgttttcctc attgtccagg aagtccttgt ccttgataat tttcagcaga
     5641 tcgtggtatg tgcccaggga ggcgttgaac cgatcttcca cgccggagat ttccacggag
     5701 tcgaagcact cgattttctt gaagtagtcc tctttcagct gcttcacggt cactttccgg
     5761 ttggtcttga acagcaggtc cacgatggcc tttttctgct cgccgctcag gaaggcgggc
     5821 tttctcattc cctcggtcac gtatttcact ttggtcagct cgttatacac ggtgaagtac
     5881 tcgtacagca ggctgtgctt gggcagcacc ttctcgttgg gcaggttctt atcgaagttg
     5941 gtcatccgct cgatgaagct ctgggcggaa gcgcccttgt ccaccacttc ctcgaagttc
     6001 cagggggtga tggtttcctc gctctttctg gtcatccagg cgaatctgct gtttcccctg
     6061 gccagagggc ccacgtagta ggggatgcgg aaggtcagga tcttctcgat cttttcccgg
     6121 ttgtccttca ggaatgggta aaaatcttcc tgccgccgca gaatggcgtg cagctctccc
     6181 aggtggatct ggtgggggat gctgccgttg tcgaaggtcc gctgcttccg cagcaggtcc
     6241 tctctgttca gcttcacgag cagttcctcg gtgccgtcca tcttttccag gatgggcttg
     6301 atgaacttgt agaactcttc ctggctggct ccgccgtcaa tgtagccggc gtagccgttc
     6361 ttgctctggt cgaagaaaat ctctttgtac ttctcaggca gctgctgccg cacgagagct
     6421 ttcagcaggg tcaggtcctg gtggtgctcg tcgtatctct tgatcataga ggcgctcagg
     6481 ggggccttgg tgatctcggt gttcactctc aggatgtcgc tcagcaggat ggcgtcggac
     6541 aggttcttgg cggccagaaa caggtcggcg tactggtcgc cgatctgggc cagcaggttg
     6601 tccaggtcgt cgtcgtaggt gtccttgctc agctgcagtt tggcatcctc ggccaggtcg
     6661 aagttgctct tgaagttggg ggtcaggccc aggctcaggg caatcaggtt tccgaacagg
     6721 ccattcttct tctcgccggg cagctgggcg atcagatttt ccagccgtct gctcttgctc
     6781 agtctggcag acaggatggc cttggcgtcc acgccgctgg cgttgatggg gttttcctcg
     6841 aacagctggt tgtaggtctg caccagctgg atgaacagct tgtccacgtc gctgttgtcg
     6901 gggttcaggt cgccctcgat caggaagtgg ccccggaact tgatcatgtg ggccagggcc
     6961 agatagatca gccgcaggtc ggccttgtcg gtgctgtcca ccagtttctt tctcaggtgg
     7021 tagatggtgg ggtacttctc gtggtaggcc acctcgtcca cgatgttgcc gaagatgggg
     7081 tgccgctcgt gcttcttatc ctcttccacc aggaaggact cttccagtct gtggaagaag
     7141 ctgtcgtcca ccttggccat ctcgttgctg aagatctctt gcagatagca gatccggttc
     7201 ttccgtctgg tgtatcttct tctggcggtt ctcttcagcc gggtggcctc ggctgtttcg
     7261 ccgctgtcga acagcagggc tccgatcagg ttcttcttga tgctgtgccg gtcggtgttg
     7321 cccagcacct tgaatttctt gctgggcacc ttgtactcgt cggtgatcac ggcccagccc
     7381 acagagttgg tgccgatgtc caggccgatg ctgtacttct tgtcagagcc gccgctgctc
     7441 tctggggttg cagattcgct tgtaccaggt gtctcgctgc cgctagagcc gccgcttctc
     7501 tcggacagtc cggcgatggc ctgtgtcacg gcttgctgca cgtcggggcc gtcctggctg
     7561 gcaggtccgg gtgtcagggt tctcagatcg gcccgctggt cggcggcatc tctataggct
     7621 ctgatgtcaa agccggtttc cttcagggtc tgcatccact cggccatctt aatctcgggg
     7681 tccacatgct gcttagactt gcgggtgtcc agagcggcca catctctgga cttcaggctg
     7741 gcgtcctctc cgacggcttc ccggattgtc tgagatctgc cgctaaaagc ttccacgggc
     7801 acgccaggca tctcccacat gccgtgcttg cccaccacct cggtttcgta tcccagggct
     7861 tccacctgct ctttcagctt ctctctgtac agtctgccga aggcgatttg attggcgtac
     7921 acgttctcga tgaatccggt ttttcccacc ttgtcgctgc tcagtgtctt ccactcgccg
     7981 ttgtgctggg tcacgttggc gaccacggcg tgggtgtgca gctgaggctc ctgatcccgg
     8041 cttgtgtcgt ggttgaagag ggccatcacc aggttgccgg tcagcactgt ctcgctctgg
     8101 ccgtcggtca tcactctggt gctagccaag gcttccacct gccgcacggc gaagtcaaca
     8161 gcctggttgt gggcgtcgat cagccgctta tctccgccca gcatagccat catggacacg
     8221 ctcttagggg cgctgaaggt caggtcgtag ccaggtctgt gccggttgct gccgtcttgc
     8281 attctgctca ggtcggcgcc atcaggaagt ctgccttcca gcagcctggt gaacacgtcc
     8341 ttatccacag agccctgcag gcccagctgt tcagctccac ggccggccca ccgttcgccc
     8401 atggagccca gcacgtagta gttatctttg tcggtgtagt aattgccggc ggagccagcg
     8461 cttctgacct gggcgatgct catcatggtg gcggctctcc ctatagtgag tcgtattagc
     8521 ggccgcggat ctctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
     8581 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     8641 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     8701 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     8761 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     8821 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     8881 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     8941 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     9001 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     9061 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     9121 ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc
     9181 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt
     9241 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata
     9301 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtcgacccta ggggatcggg
     9361 agatcgatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     9421 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9481 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9541 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9601 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9661 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9721 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9781 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9841 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9901 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9961 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10021 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10081 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10141 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10201 tattgctgat aaatctggag ccggtgagcg tggttcgcgc ggtatcattg cagcactggg
    10261 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10321 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10381 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10441 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10501 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
