LOCUS       sequence-415738-        9948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A variant CE1 construct with RepBM HUH endonuclease, expressed from 
            CMV or T7 promoters. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: HUHe variant click editor (CE1) -
            pCMV-T7-RepB...
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1215..1234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1940..1960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2031..2050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2525..5038,5039..5041,5042..6625)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2525..6625
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6683..6703
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8588..8608
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8617..8634
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8657..8674)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8663..8887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8958..8974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8958..8974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8971..8993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8982..8998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9006..9012,9013..9030,9031..9036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9051..9072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9189..9206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9360..9948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9440..9459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gcgaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgagcga gaagaaggaa atcgtgaagg gcagagattg
     2101 gaccttcctg gtgtaccccg aatctgctcc tgagaactgg cggaccatcc tcgatgagac
     2161 atttatgaga tgggtcgagt ccccactgca cgacaaggac gtgaacgccg acggcgagat
     2221 taagaaacct cattggcaca tcctgctgag cagcgacggc cccatcacac agaccgccgt
     2281 gcagaaaatc atcggccctc tgaacgcccc taatgcccag aaggtgggat ctgccaaggg
     2341 cctggttaga tatatggtgc acctggacaa ccccgaaaag taccaataca gcctggacga
     2401 gatcgtgggc cacaacggcg ccgatgtggc cagctacttc gagctgaccg cctctggcgg
     2461 aagcagcggc tccgaaaccc ctggcaccag cgagagcgct acacctgaat ccagcggagg
     2521 cagcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
     2581 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     2641 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     2701 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     2761 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     2821 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     2881 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     2941 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     3001 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     3061 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     3121 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     3181 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
     3241 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     3301 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     3361 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     3421 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     3481 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     3541 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     3601 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     3661 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     3721 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     3781 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     3841 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     3901 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     3961 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     4021 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     4081 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     4141 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     4201 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     4261 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     4321 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     4381 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     4441 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     4501 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     4561 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     4621 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     4681 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     4741 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     4801 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     4861 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     4921 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     4981 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
     5041 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     5101 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     5161 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
     5221 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     5281 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     5341 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
     5401 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     5461 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     5521 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     5581 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     5641 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     5701 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
     5761 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     5821 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
     5881 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     5941 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     6001 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     6061 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     6121 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     6181 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     6241 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     6301 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     6361 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
     6421 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     6481 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     6541 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     6601 cgacctgtct cagctgggag gtgactccgg cggaagctct ggtggcagca agcggaccgc
     6661 cgacggctct gaattcgaga gccctaagaa gaaaagaaag gtgagcggag gctctagcgg
     6721 cggaagcgtt atctcctacg acaactacgt gaccatcctg gacgaagaga cactgaaggc
     6781 ttggatcgct aaactggaaa aagcccctgt gttcgccttc gccaccgcca ccgactcctt
     6841 agataacatc agcgccaacc tggtgggcct gtctttcgcc atcgagcctg gcgtggccgc
     6901 ctacatcccc gtggcccacg attacctgga tgccccagat caaatcagca gagagagagc
     6961 actggagctc ctgaagcccc tgctggagga cgagaaggcc ctgaaggtgg gacagaacct
     7021 gaaatacgat aggggaatcc tggctaatta cggcatcgag ctgagaggca tcgccttcga
     7081 taccatgctg gaatcttaca tcctgaacag cgtggccggc cgccacgaca tggacagcct
     7141 ggctgaacgg tggctgaagc acaaaaccat caccttcgaa gagatcgccg gaaagggcaa
     7201 gaaccagctg acatttaatc agatcgcact ggaagaagcc ggcagatacg ccgctgagga
     7261 cgccgacgtg acccttcagc tgcacctgaa gatgtggccc gatctgcaga agcacaaggg
     7321 cccactaaac gtgttcgaga acatcgagat gcctctggtg cccgtcctga gcagaatcga
     7381 gaggaatggc gtgaagatcg accccaaggt gctgcacaac cacagcgagg aactgacact
     7441 gcggctggcc gagctggaaa agaaggccca cgaaatcgcc ggcgaggagt tcaacctgtc
     7501 tagcaccaag cagctacaaa caatcctgtt cgagaagcag ggcattaagc ctctgaaaaa
     7561 gacacctggc ggagccccca gcaccagcga agaagtgctg gaggaactgg cgctggatta
     7621 ccctctgccc aaagtgatcc tggaatatag aggactggcc aagctgaagt ctacatacac
     7681 cgacaagctg cctctgatga tcaaccccaa gaccggccgg gtgcatacat cttatcacca
     7741 ggccgtgaca gccaccggcc ggctgagcag cacagatcct aacctgcaaa acatcccagt
     7801 gcggaacgaa gagggcagaa gaatcagaca ggctttcatt gcccctgagg actacgtcat
     7861 cgtgtctgct gactacagcc agatagaact gagaatcatg gcccatctgt ctcgggacaa
     7921 gggcctgctg accgcttttg cagaaggcaa ggacatccac agagccaccg ctgccgaagt
     7981 gttcggcctg cctctggaaa cagtgaccag cgagcaaaga aggtccgcca aggccattaa
     8041 cttcggcctg atctacggca tgagcgcctt tggcctggcc agacagctca acatccctag
     8101 aaaggaggcc cagaaataca tggacctgta cttcgagcgg taccccggcg tgctggagta
     8161 catggaacgg accagagccc aggctaaaga gcagggatac gtggaaaccc tggatggccg
     8221 gagactgtat ctgcctgaca tcaagagcag caacggcgcc agaagagccg ccgctgagag
     8281 agctgctatc aacgccccta tgcagggcac cgccgccgac atcatcaagc gggccatgat
     8341 cgccgtggac gcctggctgc aggccgagca gcctagagtg cggatgatca tgcaggtgca
     8401 cgacgagctg gtgtttgagg tgcacaagga cgacgtggat gctgtggcca agcagattca
     8461 ccagctgatg gaaaattgca ccagactgga cgtgccactg ctggtcgagg ttggaagcgg
     8521 agagaattgg gaccaggccc actctggcgg ctcaaaaaga accgccgacg gcagcgaatt
     8581 cgagtctccc aagaagaaga ggaaagtctg accggtcatc atcaccatca ccattgagtt
     8641 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     8701 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     8761 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     8821 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     8881 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     8941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
     9061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaa
//
