LOCUS       sequence-415745-       10785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A variant CE1 construct with T4 DNA polymerase (-exo, activity 
            enhanced), expressed from CMV or T7 promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: Polymerase variant click editor (CE1) -
            pCMV-T...
FEATURES             Location/Qualifiers
     source          1..10785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1215..1234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1940..1960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2031..2050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2486..4999,5000..5002,5003..6586)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2486..6586
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6644..6664
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9425..9445
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9454..9471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9494..9511)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9500..9724
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9795..9811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9795..9811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9808..9830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9819..9835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9843..9849,9850..9867,9868..9873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9888..9909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10026..10043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10197..10785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10277..10296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gcgaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgagccc atctaagaaa aacggcagaa gcggacctca
     2101 acctcacaag cggtgggtct ttaccctgaa caaccccagc gaagatgaga gaaagaagat
     2161 ccgggacctg cctatcagcc tgttcgacta cttcatcgtg ggcgaggaag gaaatgagga
     2221 aggccggacc ccccacctgc agggcttcgc caacttcgtg aaaaagcaga ccttcaacaa
     2281 ggtgaagtgg tacctgggcg ctagatgcca catcgagaag gccaagggca ccgaccagca
     2341 gaacaaagag tactgcagca aggaaggcaa tctgctgatg gaatgtggcg cccctagatc
     2401 ccagggacag agaagcggag gctctagcgg ctccgagaca cccggcacat ctgagtccgc
     2461 cacccctgag agcagcggcg gcagcgacaa gaagtacagc atcggcctgg acatcggcac
     2521 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     2581 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     2641 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     2701 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     2761 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     2821 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     2881 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     2941 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     3001 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     3061 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     3121 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     3181 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     3241 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     3301 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     3361 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     3421 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     3481 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     3541 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     3601 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     3661 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     3721 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     3781 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     3841 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     3901 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     3961 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     4021 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     4081 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     4141 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     4201 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     4261 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     4321 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     4381 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     4441 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     4501 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     4561 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     4621 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     4681 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     4741 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     4801 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     4861 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     4921 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     4981 gtccgactac gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga
     5041 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     5101 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     5161 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     5221 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     5281 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     5341 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     5401 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     5461 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     5521 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     5581 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     5641 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     5701 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     5761 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     5821 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     5881 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     5941 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     6001 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     6061 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     6121 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     6181 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     6241 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     6301 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     6361 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     6421 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     6481 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     6541 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactccg gcggaagctc
     6601 tggtggcagc aagcggaccg ccgacggctc tgaattcgag agccctaaga agaaaagaaa
     6661 ggtgagcgga ggctctagcg gcggaagcaa ggaattctac atcagcatcg aaaccgtggg
     6721 caacaacatc gttgagagat acatcgacga gaacggcaag gaaagaacca gagaggtgga
     6781 atacctgcct acaatgttcc ggcactgtaa ggaagagtct aagtacaagg acatctacgg
     6841 aaagaattgc gcccctcaga agttccccag catgaaggac gccagagatt ggatgaagag
     6901 aatggaggac attggcctgg aagccctggg catgaacgac ttcaagctgg cctacatatc
     6961 tgatacctac ggctctgaga tcgtgtacga cagaaagttc gtgcgggtgg ccaactgcga
     7021 catcgaggtg acaggcgata agtttccaga tcctatgaag gcagagtatg aaatcgacgc
     7081 catcacacac tacgacagca ttgacgatag attttacgtg ttcgatctgc tgaacagcat
     7141 gtacggcagc gtgagcaagt gggacgccaa actggccgcc aagctggact gcgaaggcgg
     7201 cgatgaggtg ccacaggaga tcctggaccg ggtgatctac atgcccttcg cgaacgcgcg
     7261 ggacatgctg atggagtaca tcaatctgtg ggagcagaaa cgtccagcca tcttcaccgg
     7321 gtggaatatc gagggcttcg acgtgcctta catcatgaac cgggtgaaga tgatcctggg
     7381 cgagcgctct atgaagagat tctccccaat cggcagagtg aagtccaagc tgatccaaaa
     7441 catgtatgga agcaaggaaa tctacagcat cgacggcgtc agcatcctgg actacctgga
     7501 cctctacaag aaattcgcct ttaccaacct gcctagcttt tccctggaaa gcgtggccca
     7561 acacgaaacc aagaagggca agctgcccta cgacggccct attaacaaac tgagagagac
     7621 aaatcaccag cggtacatca gctacaacat catcgatgtg gagtctgtgc aggccatcga
     7681 caagatcagg ggcttcatcg acctggtgct gtccatgagc tactacgcca agatgccttt
     7741 cagcggcgtg atgagcccta tcaaaacatg ggatgccatc atttttaaca gcctgaaggg
     7801 agaacacaag gtgatccccc agcagggctc ccacgtgaaa cagtccttcc ccggagcttt
     7861 tgtttttgag cctaagccca tcgccagaag atacattatg agcttcgacc tgacaagcat
     7921 gtaccctagc atcatcagac aggtgaacat ctctcccgag acaatcagag gccagttcaa
     7981 ggtgcacccc atacacgagt acatcgctgg caccgcccca aaaccatctg acgaatatag
     8041 ctgttctcct aacggatgga tgtacgacaa gcatcaggag ggtatcatcc ctaaggaaat
     8101 cgccaaggtt ttcttccaga gaaaagactg gaagaagaag atgttcgccg aggaaatgaa
     8161 cgccgaagcc attaagaaga tcatcatgaa gggcgccgga agctgcagca ccaagcctga
     8221 ggtggagcgc tatgtgaaat tcagcgatga tttcctgaac gagctgagca actacaccga
     8281 gagcgtgctg aacagcttga tcgaggagtg tgagaaggcc gctacactgg ccaacaccaa
     8341 ccagctgaat agaaagatcc ttattaacag cctgtacggc gctttgggaa acatccattt
     8401 ccggtactac gacctccgga acgccaccgc catcaccatt ttcggacagg tgggcatcca
     8461 gtggatcgcc agaaaaatca acgagtacct gaacaaagtg tgcggcacca atgatgagga
     8521 cttcatcgct gccggcgata ctgatagcgt gtacgtgtgc gtggacaagg ttatcgagaa
     8581 ggtgggcctg gaccggttca aggaacagaa cgacctggtg gaattcatga accagtttgg
     8641 gaaaaaaaag atggaaccta tgatcgatgt ggcttataga gagctgtgcg actacatgaa
     8701 caatagagag cacctgatgc acatggaccg ggaggccatc agttgtcctc ctctgggcag
     8761 caagggcgtc ggcggattct ggaaggccaa gaagcggtac gccctgaacg tgtacgacat
     8821 ggaagataag agattcgctg aaccccacct gaaaatcatg ggcatggaaa cacagcagag
     8881 cagcacccct aaggccgtgc aggaggccct ggaggaatct atcagacgga tcctgcagga
     8941 aggcgaagaa tctgtgcagg agtattacaa gaacttcgag aaggagtaca gacaactgga
     9001 ttacaaggtg atcgctgagg tgaagaccgc caatgacatc gctaagtacg acgacaaggg
     9061 ctggcctggc tttaagtgcc ccttccacat cagaggtgtg ctgacctacc gcagagccgt
     9121 gagtggcctg ggcgtggccc ccatcctgga tggcaacaaa gtgatggtgc tgcctctgcg
     9181 ggagggaaat cctttcggcg acaaatgcat cgcctggcct tctggcaccg agctccctaa
     9241 ggaaatcaga agcgacgtgc tgagctggat cgaccacagc accctgttcc aaaagtcttt
     9301 cgtgaagcct ctggccggca tgtgcgagtc cgccggcatg gactacgagg aaaaagcttc
     9361 tctggacttc ctgttcggct ctggcggctc aaaaagaacc gccgacggca gcgaattcga
     9421 gtctcccaag aagaagagga aagtctgacc ggtcatcatc accatcacca ttgagtttaa
     9481 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     9541 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     9601 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     9661 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     9721 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     9781 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9841 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     9901 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9961 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10621 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
