LOCUS       sequence-415797-       10616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrates a T7 RNA polymerase, tetracycline repressor and an 
            mNeongreen Lac repressor fusion protein with a puromycin drug 
            selection marker into the tubulin locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Budzak J, Goodwin I, Tiengwe C, Rudenko G
  TITLE     Imaging of genomic loci in Trypanosoma brucei using an optimised 
            LacO-LacI system.
  JOURNAL   Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 
            10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3.
  PUBMED    37923299
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biochem
            Parasitol."; date: "2023-12"; volume: "256:111598. doi"; pages: " 
            10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3"
COMMENT     Sequence Label: pSMOX-mNG::LacI-V3
FEATURES             Location/Qualifiers
     source          1..10616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3315..3914
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3315..3334)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3811..3831
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6053..6072)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             7114..7134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7634..7653)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7863..7882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7982..8004
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8042..8060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8128..8232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8233..8301,8302..9093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8451..8470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9264..9852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9753..9772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10006..10023
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 catttatgtt ctttctttct ttcttttgtg aatttgtttt ctgtctcaaa tgtttttaat
       61 tcgcttggga cctatgtttt tcttgttttt ttgctcaccc tttgtgtagg aggcaccctg
      121 tcacgtctgt ggttgcgtgt atgccttcct tccccttatt cgcttcttcc tgtcgtgtca
      181 cacctctttc tctctctccc tttccgcctt ttctttcaat cttgttttct cgaccagccc
      241 tactagagga gaaagaatag taaccctttc atcaaagaaa atagttcaaa cgaattacta
      301 gtatgcgcgg ccacaagcgc tcgcgcgagt tctctgacat cgagctggcc gcgatcccgt
      361 tcaacacgct ggcggaccac tacggcgagc gcctcgcgag agagcagctg gcgctggaac
      421 atgagagcta cgagatgggc gaggcccgct tccgcaagat gtttgagcgg cagctgaagg
      481 cgggcgaggt ggcggacaac gcggcggcga agccgctgat taccacgctg ctgccaaaga
      541 tgatcgcgcg catcaacgac tggttcgagg aggtgaaggc gaagcgcgga aagcgtccga
      601 cggcgttcca gttcctgcag gagattaagc cggaggcggt ggcctacatc acgatcaaga
      661 cgaccctggc ttgcctcacc tcggccgaca acactactgt ccaggcggtg gcctcggcga
      721 ttggccgtgc gattgaggac gaggcgcggt tcggccgcat ccgcgacctg gaggcgaagc
      781 acttcaagaa gaacgtggag gagcaactga acaagcgagt gggccacgtg tacaagaagg
      841 cgttcatgca ggtcgtggag gcggacatgc tgagcaaagg cctgctgggc ggcgaggcgt
      901 ggtcatcgtg gcacaaggag gacagcatcc acgttggcgt ccgctgcatc gagatgctga
      961 tcgagtcgac gggcatggtg tctctgcacc gtcagaacgc cggcgtggtg ggtcaggaca
     1021 gcgagacgat cgagctggcg ccggagtacg cagaggcgat cgcgacccgt gccggcgcgc
     1081 tggcgggcat cagcccgatg ttccagccgt gcgttgtgcc gccgaagcct tggactggca
     1141 tcaccggcgg gggctactgg gcgaacggtc gccgcccgct ggcgctggtg cgtacgcact
     1201 ccaaaaaggc gctgatgcgc tacgaggacg tgtacatgcc agaggtgtac aaggcgatca
     1261 acatcgcgca gaacaccgcg tggaagatca acaagaaggt gctggcggtg gcgaacgtaa
     1321 tcacgaagtg gaagcactgt ccggtggagg atatcccggc gatcgagcgt gaggagctgc
     1381 cgatgaagcc ggaggacatc gacatgaacc cggaggcgct gaccgcctgg aagcgcgccg
     1441 cggctgcggt gtaccgcaag gaccgcgcgc ggaagtcgcg ccgcatctcc ctggagttca
     1501 tgctcgagca ggcgaataaa ttcgcgaacc acaaggccat ttggtttccc tataacatgg
     1561 actggcgcgg tcgcgtgtac gcggtctcga tgttcaaccc gcagggcaac gacatgacca
     1621 agggcctgct tacgctggcg aagggcaagc cgatcggcaa ggagggctac tactggctca
     1681 agatccacgg tgcgaactgc gcgggcgtgg acaaggtgcc gttcccggag aggatcaagt
     1741 tcattgagga gaaccacgag aatatcatgg cgtgcgcgaa gagcccgctg gagaacacct
     1801 ggtgggcgga gcaggacagc cctttctgct tcctggcgtt ctgcttcgag tacgccggcg
     1861 tgcagcacca cggcctctcc tacaactgct ccctgccact ggcgttcgat gggtcctgct
     1921 ctggcatcca gcacttctct gcgatgctgc gtgacgaggt gggtggccgc gcagtgaacc
     1981 tgctgccgag cgagacggtg caggatatct acggcatcgt ggcgaagaag gtgaacgaga
     2041 tcctgcaggc ggacgcgatc aatggcacgg acaacgaggt ggtgacggtg accgacgaga
     2101 acaccggcga gatctccgaa aaggtcaagc tgggcacgaa ggcgctggcg ggtcagtggc
     2161 tcgcgcacgg cgtgacccgc tcggtaacga agcgctcggt gatgacgctg gcgtacggct
     2221 cgaaggagtt cggcttccgc cagcaggtgc tcgaggacac gatccagccg gcgatcgaca
     2281 gcggtaaggg ccccatgttc acgcagccga accaggcggc gggctacatg gcgaagctga
     2341 tctgggagtc ggttagcgtc acggtggtgg cggcggtcga ggcgatgaac tggctcaaga
     2401 gcgcggccaa gctgctggcg gcggaggtaa aggacaagaa gaccggcgag atcctgcgca
     2461 agcgctgcgc ggtgcactgg gtgacgccgg acggcttccc cgtgtggcag gagtacaaga
     2521 agccgatcca gacgcgcctg aatctgatgt tcctcggcca gttccgcctg cagccgacga
     2581 tcaacaccaa caaggacagc gagattgatg cgcacaagca agagtcgggc atcgcaccga
     2641 acttcgtgca cagccaggac ggctcccacc ttcgcaagac ggtggtctgg gcgcacgaga
     2701 aatacggcat cgagtctttc gcacttatcc acgactcttt cggcacgatc cccgcggacg
     2761 cagccaacct gtttaaggcg gtgcgcgaga cgatggtgga tacgtacgag agctgcgacg
     2821 tgcttgcgga cttttacgac cagttcgccg accagctgca cgagtctcag ctggacaaga
     2881 tgcctgccct cccggccaag ggcaacctga accttcgcga catcctcgag tctgacttcg
     2941 cgttcgcgta aggatcccgc tgcgcttaag tgtctttcat tataatcaat gtataacctt
     3001 tatgtagtat ttcaatctat gccgctgtgt acgtgcactg cggtgcctat ccttcggcat
     3061 tagagagtca ctgtttgtgt agatcgtagc tgcatgtctg acatctggtt cattagtgcc
     3121 gttttgagtc tgcactgttg tgcaacttga tatgcatcac tgcacaccaa ccgtgtgttt
     3181 cctcctttgg gtgcaccctt taatccttgt tttctccttt tttgtctctt tccccctcga
     3241 aaaggtgtca aactactgcc gcataaacta cggttatccc aaatttaaga gaaagcaata
     3301 aagcatcaga attcatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     3361 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     3421 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     3481 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     3541 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     3601 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     3661 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     3721 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     3781 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     3841 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     3901 agcccggtgc ctaaccatgg cgctgcgctt aagtgtcttt cattataatc aatgtataac
     3961 ctttatgtag tatttcaatc tatgccgctg tgtacgtgca ctgcggtgcc tatccttcgg
     4021 cattagagag tcactgtttg tgtagatcgt agctgcatgt ctgacatctg gttcattagt
     4081 gccgttttga gtctgcactg ttgtgcaact tgatatgcat cactgcacac caaccgtgtg
     4141 tttcctcctt tgggtgcacc ctttaatcct tgtcttctcc ttttttgtct ctttccccct
     4201 cgaaaaggtg tcaaactact gccgcataaa ctacggttat cccaaattta agagaaagca
     4261 ataaagcatc acctagggat aacagggtaa tgcatgcaag ctaggatgcg cggccacaag
     4321 cgctccaggg agcgcctgga caagagcaag gtcattaaca gcgcgctcga gctgctcaac
     4381 gaggtgggca ttgagggtct caccacgcgc aagctcgcgc agaagctcgg cgtcgagcag
     4441 cccacgctct actggcacgt gaagaacaag cgcgcgctcc tcgacgcgct ggcgatcgag
     4501 atgctggatc gccaccacac ccacttttgc cccctcgagg gtgagagctg gcaggacttt
     4561 ctccgcaaca acgcgaagag ctttcgctgc gccctgctca gccaccgcga cggcgccaag
     4621 gtgcacctgg gtacccgccc gacggagaag cagtacgaga cgctcgagaa ccagctggcg
     4681 ttcctctgcc agcagggctt ttcgctggag aacgccctgt acgccctgtc ggcggtcggt
     4741 cacttcaccc tcggttgcgt cctggaggac caggagcacc aggtggccaa ggaggagcgc
     4801 gagaccccga ccaccgacag catgcccccc ctcctccgcc aggcgattga gctgttcgat
     4861 caccagggtg ccgagccggc gttcctgttc ggcctggagc tgattatttg cggtctggag
     4921 aagcagctca agtgcgagtc gggttcgtaa gagctcctgc gcttaagtgt ctttcattat
     4981 aatcaatgta taacctttat gtagtatttc aatctatgcc gctgtgtacg tgcactgcgg
     5041 tgcctatcct tcggcattag agagtcactg tttgtgtaga tcgtagctgc atgtctgaca
     5101 tctggttcat tagtgccgtt ttgagtctgc actgttgtgc aacttgatat gcatcactgc
     5161 acaccaaccg tgtgtttcct cctttgggtg caccctttaa tccttgtctt ctcctttttt
     5221 gtctctttcc ccctcgaaaa ggtgtcaaac tactgccgca taaactacgg ttatcccaaa
     5281 tttaagagaa agcaataaag catcacctct agatagatgg ccagcttgcc agcaacgcac
     5341 gagcttcata tatttgggtc gatcaacggt gtggatttcg acatggtggg tcaaggcaca
     5401 ggtaatccta atgacgggta tgaagaactc aatttgaaga gcactaaggg agatctccag
     5461 ttttccccgt ggattcttgt gccgcatatt ggttatggtt ttcatcagta cttaccatac
     5521 cccgatggaa tgtcgccctt ccaagcagct atggtggacg gctcggggta tcaagtacat
     5581 cgcaccatgc aatttgagga cggagcatcc ctcaccgtaa actaccgcta tacgtatgag
     5641 gggagccaca taaaaggtga ggctcaagtt aaaggcacag gcttcccagc tgacggcccg
     5701 gtcatgacca attcattaac cgctgctgat tggtgccgtt ctaagaagac ctaccctaac
     5761 gacaagacga tcattagtac ttttaagtgg agttatacca caggaaacgg aaagcggtat
     5821 cgaagcactg ctcgaacaac ctacacattt gcgaagccaa tggccgcgaa ctacttgaag
     5881 aaccagccaa tgtatgtgtt tcgaaaaacg gaactgaagc attcgaaaac cgagcttaat
     5941 tttaaagagt ggcagaaggc gtttacagac gtgatgggga tggacgaatt gtataaggga
     6001 tccggatcag gatctggaac atctcgaggg atggtgaaac cagtaacgtt atacgatgtc
     6061 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
     6121 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
     6181 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
     6241 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
     6301 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
     6361 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
     6421 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
     6481 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
     6541 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
     6601 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
     6661 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
     6721 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
     6781 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
     6841 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
     6901 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     6961 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     7021 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7081 gcacgacagg tttcccgaag cagcctgagg cctcctaaga agaagaggaa ggtttgaggc
     7141 tagcacgcgg acggggcatt tcccgttcgt cattagcagt aggtaatgaa gatgtttgtt
     7201 tctcgtcccc tttctccttc gtccttctgt cattttgttc ttttgtgttt atgttttgtt
     7261 gttgttttct ttaattattt ttttttcttc cacgtttgtg tacatccgcg cgccactcta
     7321 ttcagagagc cacggatagt agaggaggtg ggaagggtat atgagggaca cgcgtaccat
     7381 gatgtgggat gtattggggt ccctgtctgt ccttacgtga ctatgtatga accgtcacgt
     7441 gtaagatgag ctagtgagat caacagtaca actcatcaac acgccttctt ctcgttaaat
     7501 gtacacaatc ttgatcctcc acctttatgg gtcccattgt ttgcctcttc cgctgtgaag
     7561 ctttctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     7621 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     7681 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     7741 ttgggcgcca tctccttgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     7801 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     7861 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     7921 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7981 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     8041 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8461 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9961 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10021 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10081 taatgcagtc gagggtgctc aagctgtgta gcgcacgcgt aagcttgtgt ttataatatc
    10141 gttctttttt atatcagtgt aacgattgct cacacttatt taggatcaaa tttcacttac
    10201 tttcaaattt gtgttgtaac aatatagaaa ggaaaagcaa gtcattcaag catgataagg
    10261 aaccttcagc agcgccactg cgttaacttc cactcaacat aataaaataa taaagatatt
    10321 tgaatttaaa ataaacttac ctttcaaaaa tcgcagaaaa acacttgaac ggaacgaaaa
    10381 aataaaaaaa acatgccatc accatatcaa caacatcacc agaaaaaaat aaaataaaac
    10441 cttatttaag gacgaaaagt agatacctat cattaaacaa aataaaaaaa tctatcaaca
    10501 tataacataa cataagaaat aaaacaataa ctataataga acaatcacaa atattattga
    10561 atctctccct ctccatatat atatatatat ataccttcgc accatgtgta ggtttt
//
