LOCUS       sequence-415820-        9920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein quality control substrate and associated control YIp 
            ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Flagg MP, Lam B, Lam DK, Le TM, Kao A, Slaiwa YI, Hampton RY
  TITLE     Exploring the ""misfolding problem"" by systematic discovery and 
            analysis of functional-but-degraded proteins.
  JOURNAL   Mol Biol Cell. 2023 Dec 1;34(13):ar125. doi: 
            10.1091/mbc.E23-06-0248. Epub 2023 Sep 20.
  PUBMED    37729018
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2023-12-1"; volume: "34(13):ar125. doi"; pages: " 
            10.1091/mbc.E23-06-0248. Epub 2023 Sep 20"
COMMENT     Sequence Label: pRH3142
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..669
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     562..585
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(701..717)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(702..718)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             1519..2229
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1546..1574)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2169..2189
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2253..2440
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2308..2332)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2444..2463)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2445..2463)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2473..2490)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2473..2489)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2482..2504)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2630..3085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2717..2736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2927..2948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3290..5005)
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     promoter        complement(5006..5381)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(5572..5591)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5691..5713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5751..5769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(6055..7149)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7150..7557)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        7725..7829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7830..7898,7899..8690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8048..8067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8861..9449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9350..9369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9603..9620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9737..9758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9773..9778,9779..9796,9797..9803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9811..9827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9816..9838
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9835..9851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9835..9851
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9870..9890
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9872..9890
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccgctgct tggcggtcga gtttatcatt atcaatactg ccatttcaaa gaatacgtaa
       61 ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct
      121 gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag
      181 gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa
      241 gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat
      301 cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa
      361 aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca
      421 attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc
      481 tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact
      541 tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta
      601 aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac accaagaact
      661 tagtttcgac ggatctgcag gaattcgata tcaagcttat cgataccgtc gacctcgaga
      721 gatatggatt tcacaaaacc agaaactgtt ttaaatctac aaaatattag agatgaatta
      781 gttagaatgg aggattcgat catcttcaaa tttattgaga ggtcgcattt cgccacatgt
      841 ccttcagttt atgaggcaaa ccatccaggt ttagaaattc cgaattttaa aggatctttc
      901 ttggattggg ctctatcaaa tcttgaaatt gcgcattctc gcatcagaag attcgaatca
      961 cctgatgaaa ctcccttctt tcctgacaag attcagaaat cattcttacc gagcattaac
     1021 tacccacaaa ttttggcgcc ttatgcccca gaagttaatt acaatgataa aataaaaaaa
     1081 gtttatattg aaaagattat accattaatt tcgaaaagag atggtgatga taagaataac
     1141 ttcggttctg ttgccactag aggtatagaa tgtttgcaaa gcttgagtag gagaatccac
     1201 tttggcaagt ttgttgctga agccaagttc caatcggata tcccgctata cacaaagctg
     1261 atcaaaagta aagatgtcga ggggataatg aagaatatca ccaattctgc cgttgaagaa
     1321 aagattctag aaagattaac taagaaggct gaagtctatg gtgtggaccc taccaacgag
     1381 tcaggtgaaa gaaggattac tccagaatat ttggtaaaaa tttataagga aattgttata
     1441 cctatcacta aggaagttga ggtggaatac ttactaagaa ggttggaaga gggtcgacgg
     1501 atccccgggt taattaacag taaaggagaa gaacttttca ctggagttgt cccaattctt
     1561 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     1621 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1681 ccatggccaa cacttgtcac tactttcact tatggtgttc aatgcttttc aagataccca
     1741 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     1801 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     1861 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     1921 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     1981 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     2041 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2101 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     2161 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2221 ctatacaaat agggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
     2281 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     2341 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     2401 tatagtatga ggtcgctctt attgaccaca cctctaccgg cagccctata gtgagtcgta
     2461 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2521 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2581 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     2641 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2701 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2761 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2821 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2881 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2941 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3001 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3061 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     3121 gtatttcaca ccgcatagat cttatgtatg aaattcttaa aaaaggacac ctgtaagcgt
     3181 tgatttctat gtatgaagtc cacatttgat gtaatcataa caaagcctaa aaaataggta
     3241 tatcatttta taattatttg ctgtacaagt atatcaataa acttatatat tacttgtttt
     3301 ctagataagc ttcgtaaccg acagtttcta acttttgtgc tttgacaaga acttcttctt
     3361 cttgctttaa taaaaactgt tccattttcg ttgtataact tgaatcataa gcgccaagca
     3421 gtctgacagc caacagcgca gcgttcgtac tattattaat agcgacggta gctactggaa
     3481 cacctctagg catttgcaca attgaatgta aagaatctac tccatctaga caagaacctt
     3541 ttacgggcac accgatgaca ggaagtggtg tcattgcagc caccatacct ggcaagtgag
     3601 cagccccacc agctccagcg ataattgttt taattccacg cttgcttgcg gaaatagcat
     3661 atgctgacat cctatgtgga gttctatgag cagagactat tgtcacttca aatggaacgc
     3721 caaaatcttt taaaaccgca catgcggcag acattaccgg caagtcagag tctgatccca
     3781 tgatgattcc aaccaatggt ttgaccattg cttccaagtc caacttttga gcgacagaga
     3841 ttttgattgg aatatcagtt ctacctgtaa tgtagttcag cctttgttca cattccgcca
     3901 tactggaggc aataatattt atgtgaccta cttttctgtt aggtctagac tcttttccat
     3961 ataagtacac tgaggaacct ggagtcgcca atgctctttc gcaagtttct agctctttat
     4021 cttttgtatg tttgtctcca agaacattta gcataatggc gttcgttgta atggtggaga
     4081 aagatgtgaa attctttggc attggcaaat ccaatattga tctcaaatga gcttcaaatt
     4141 gagaagtgac gcaagcatca atggtataat gtccagagtt gtgaggcctt ggggcaattt
     4201 cgttaataag caattcccct gtttctaaat agaacatttc cacaccaaat ataccacaac
     4261 cgggaaaaga tttgattgca ttttctgcca acaacttcgc cttaagttga acggagtccg
     4321 gaactctagc aggcgcataa cataagtcac aaatattgtc cttgtggata gtctctacaa
     4381 ttgggtaaga aaacactaaa ccgttaacag acctcacaat catgactgct aattctttag
     4441 taaatggtgc ccatttttcg gcgtacaaag gacgatcctt cagtacttcc aaagcttccg
     4501 gaatcatttc cttattcttt acaacgaagt tacctcttcc atcgtatgcc aaagtcctcg
     4561 acttcaagac gaatggaaaa cccaaatctc ttccaacatt caatagggac gtctcactgg
     4621 cttgttccac aggaacactt tgggtaactg ctataccatt tttgattaaa tgctcttttt
     4681 gaatatattt gtcttgtatc aatctgattg tttctggaga agggtaaatt tttaatttgg
     4741 gatgttttac ttgaagattc tttagtgtag gaacatcaac atgctcaatc tcaatcgtta
     4801 gcacatcaca tttttcagct agtttttcga tatcaagagg attggaaaag gagccattaa
     4861 cgtggtcatt ggagttgctt atttgtttgg caggagaatt ttcagcatct agtattaccg
     4921 tcttaatgtt gagcctgttt gctgcctcaa caatcatacg tcccaattgt ccccctccta
     4981 atataccaac tgttctagaa tccatacttg attgttttgt ccgattttct tgtttttctt
     5041 gattgttata gtaggatgta cttagaagag agatccaacg attttacgca ccaatttata
     5101 catgaaatgc tccataatat tgtccattta gttcttaata aaaggtcagc aagagtcaat
     5161 cacttagtat tacccggttc gtagccatgc aacaagagtc atttgtcagc atagctgtaa
     5221 taatcaatca tgacgtaaga aatgtatcat aattaaaagt tgttaaagat gtcagtgtta
     5281 tgttggtgtt acaaaattct cggcttctca ctaatattta atatctctta aattttatct
     5341 gtctttgatt cttttaagaa aagttatgta ttattcaaga attaattcag atctattctt
     5401 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5461 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     5521 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     5581 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5641 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5701 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5761 acgcctattt ttataggtta atgtcatgat ctttttgtgt ggtgccctcc tccttgtcaa
     5821 tattaatgtt aaagtgcaat tctttttcct tatcacgttg agccattagt atcaatttgc
     5881 ttacctgtat tcctttacat cctccttttt ctccttcttg ataaatgtat gtagattgcg
     5941 tatatagttt cgtctaccct atgaacatat tccattttgt aatttcgtgt cgtttctatt
     6001 atgaatttca tttataaagt ttatgtacaa atatcataaa aaaagagaat ctttttaagc
     6061 aaggattttc ttaacttctt cggcgacagc atcaccgact tcggtggtac tgttggaacc
     6121 acctaaatca ccagttctga tacctgcatc caaaaccttt ttaactgcat cttcaatggc
     6181 cttaccttct tcaggcaagt tcaatgacaa tttcaacatc attgcagcag acaagatagt
     6241 ggcgataggg ttgaccttat tctttggcaa atctggagca gaaccgtggc atggttcgta
     6301 caaaccaaat gcggtgttct tgtctggcaa agaggccaag gacgcagatg gcaacaaacc
     6361 caaggaacct gggataacgg aggcttcatc ggagatgata tcaccaaaca tgttgctggt
     6421 gattataata ccatttaggt gggttgggtt cttaactagg atcatggcgg cagaatcaat
     6481 caattgatgt tgaaccttca atgtagggaa ttcgttcttg atggtttcct ccacagtttt
     6541 tctccataat cttgaagagg ccaaaacatt agctttatcc aaggaccaaa taggcaatgg
     6601 tggctcatgt tgtagggcca tgaaagcggc cattcttgtg attctttgca cttctggaac
     6661 ggtgtattgt tcactatccc aagcgacacc atcaccatcg tcttcctttc tcttaccaaa
     6721 gtaaatacct cccactaatt ctctgacaac aacgaagtca gtacctttag caaattgtgg
     6781 cttgattgga gataagtcta aaagagagtc ggatgcaaag ttacatggtc ttaagttggc
     6841 gtacaattga agttctttac ggatttttag taaaccttgt tcaggtctaa cactaccggt
     6901 accccattta ggaccaccca cagcacctaa caaaacggca tcagccttct tggaggcttc
     6961 cagcgcctca tctggaagtg gaacacctgt agcatcgata gcagcaccac caattaaatg
     7021 attttcgaaa tcgaacttga cattggaacg aacatcagaa atagctttaa gaaccttaat
     7081 ggcttcggct gtgatttctt gaccaacgtg gtcacctggc aaaacgacga tcttcttagg
     7141 ggcagacatt agaatggtat atccttgaaa tatatatata tatattgctg aaatgtaaaa
     7201 ggtaagaaaa gttagaaagt aagacgattg ctaaccacct attggaaaaa acaataggtc
     7261 cttaaataat attgtcaact tcaagtattg tgatgcaagc atttagtcat gaacgcttct
     7321 ctattctata tgaaaagccg gttccggcgc tctcaccttt cctttttctc ccaatttttc
     7381 agttgaaaaa ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc
     7441 gaatttgatt ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa
     7501 tggttgctaa gagattcgaa ctcttgcatc ttacgatacc tgagtattcc cacagttaac
     7561 tgcggtcaag atatttcttg aatcaggcgc cttagaccgc tcggccaaac aaccaattac
     7621 ttgttgagaa atagagtata attatcctat aaatataacg tttttgaaca cacatgaaca
     7681 aggaagtaca ggagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7741 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7801 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7861 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7921 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7981 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8041 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8101 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8161 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8221 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8281 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8341 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8401 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8461 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8521 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8581 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8641 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8701 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8761 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8821 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8881 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8941 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9001 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9061 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9121 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9181 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9241 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9301 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9361 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9421 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9481 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9541 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9601 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9661 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9721 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9781 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9841 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     9901 tggagctcca ccgcggtggc
//
