LOCUS       sequence-415832-        9608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEST vector to tag gene of interest with DHFR F[3] on the 
            C-terminus to perform DHFR-PCA in S. cerevisiae. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pKB12 (pDEST-DHFR F[3] C-term, HygR)
FEATURES             Location/Qualifiers
     source          1..9608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    834..958
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1132..1234
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1235..1915
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWS*YHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1301..1320)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2235..2540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2419..2438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2581..2705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3203..3390
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3258..3282)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3542..3561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3543..3561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3568..3585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3568..3584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3577..3599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3725..4180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3812..3831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4022..4043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4319..4662
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4663..5691
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             4663..5691
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      5699..5946
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5715..5733)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5968..5987)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6087..6109
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6147..6165)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6207..6710
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6747..6851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6852..6920,6921..7712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7070..7089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7883..8471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8372..8391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8625..8642
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8759..8780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8795..8800,8801..8818,8819..8825)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8833..8849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8838..8860
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8857..8873
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8857..8873
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8894..8912
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atgtaactat aacggtccta aggtagcgaa attaaagtcc aatgctagta gagaaggggg
       61 gtaacacccc tccgcgctct tttccgattt ttttctaaac cgtggaatat ttcggatatc
      121 cttttgttgt ttccgggtgt acaatatgga cttcctcttt tctggcaacc aaacccatac
      181 atcgggattc ctataatacc ttcgttggtc tccctaacat gtaggtggcg gaggggagat
      241 atacaataga acagatacca gacaagacat aatgggctaa acaagactac accaattaca
      301 ctgcctcatt gatggtggta cataacgaac taatactgta gccctagact tgatagccat
      361 catcatatcg aagtttcact accctttttc catttgccat ctattgaagt aataataggc
      421 gcatgcaact tcttttcttt ttttttcttt tctctctccc ccgttgttgt ctcaccatat
      481 ccgcaatgac aaaaaaatga tggaagacac taaaggaaaa aattaacgac aaagacagca
      541 ccaacagatg tcgttgttcc agagctgatg aggggtatct cgaagcacac gaaacttttt
      601 ccttccttca ttcacgcaca ctactctcta atgagcaacg gtatacggcc ttccttccag
      661 ttacttgaat ttgaaataaa aaaaagtttg ctgtcttgct atcaagtata aatagacctg
      721 caattattaa tcttttgttt cctcgtcatt gttctcgttc cctttcttcc ttgtttcttt
      781 ttctgcacaa tatttcaagc tataccaagc atacaaaggg tgggtcgaat caaacaagtt
      841 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
      901 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc
      961 ggccgcgggt gatgctgcca acttagcggc cgctaagttg gcagcatcac ccgacgcact
     1021 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1081 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     1141 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1201 gtcatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1261 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1321 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1381 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1441 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1501 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagttaata
     1561 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1621 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1681 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1741 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1801 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1861 acagtactgc gatgagtggc aggcggggcg taatctagag gatccggctt actaaaagcc
     1921 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     1981 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     2041 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     2101 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     2161 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     2221 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     2281 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     2341 tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg gtggtgcata
     2401 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     2461 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     2521 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     2581 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     2641 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     2701 gtggtaggag gaggaagtac tagtatgagt aaagtagaca tggtttggat agtcggaggc
     2761 agttctgttt accaggaagc catgaatcaa ccaggccacc tcagactctt tgtgacaagg
     2821 atcatgcagg aatttgaaag tgacacgttt ttcccagaaa ttgatttggg gaaatataaa
     2881 cttctcccag aatacccagg cgtcctctct gaggtccagg aggaaaaagg catcaagtat
     2941 aagtttgaag tctacgagaa gaaagactaa tttaagtaag taatggcaaa cagctattat
     3001 gggtattatg ggttagggat aacagggtaa taagctctaa gtaagtaacg gccgccaccg
     3061 cggtggagct ttggacttct tcgccagagg tttggtcaag tctccaatca aggttgtcgg
     3121 cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata
     3181 cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata
     3241 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     3301 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga
     3361 ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc
     3421 ccatttcacc caattgtaga tatgctaact ccagcaatga gttgatgaat ctcggtgtgt
     3481 attttatgtc ctcagaggac aacacctgtt gtaatcgttc ttccacacgg atcccaattc
     3541 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     3601 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3661 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3721 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3781 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3961 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4021 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4081 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4141 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     4201 ttacgcatct gtgcggtatt tcacaccgcg tacgctgcag gtcgacggat ccccgggtta
     4261 attaaggcgc gccagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga
     4321 catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg
     4381 tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg catccataca
     4441 ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcgga cctgcgagca
     4501 gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa
     4561 tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt
     4621 gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa aaagcctgaa
     4681 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     4741 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     4801 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     4861 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     4921 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     4981 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     5041 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     5101 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     5161 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     5221 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     5281 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     5341 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     5401 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     5461 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     5521 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     5581 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     5641 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata atctcgagtc
     5701 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa
     5761 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
     5821 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac
     5881 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta
     5941 acgagctcga attcatcgat ctctcagtac aatctgctct gatgccgcat agttaagcca
     6001 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     6061 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     6121 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6181 catgataata atggtttctt aggacggatc gcttgcctgt aacttacacg cgcctcgtat
     6241 cttttaatga tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta
     6301 tttggatttt agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat
     6361 aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa
     6421 gaaaagcaga ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg
     6481 attttcgtgt gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga
     6541 gcaggaagag caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc
     6601 ttcggaaaac aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta
     6661 tatatttata ttaaaaaatt taaattataa ttatttttat agcacgtgat gaaaaggacc
     6721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7081 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7201 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7261 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7321 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7381 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7441 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7501 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7561 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7621 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7681 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8521 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8581 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8641 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8701 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8761 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8821 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     8881 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct
     8941 cgagatccgg gatcgaagaa atgatggtaa atgaaatagg aaatcaagga gcatgaaggc
     9001 aaaagacaaa tataagggtc gaacgaaaaa taaagtgaaa agtgttgata tgatgtattt
     9061 ggctttgcgg cgccgaaaaa acgagtttac gcaattgcac aatcatgctg actctgtggc
     9121 ggacccgcgc tcttgccggc ccggcgataa cgctgggcgt gaggctgtgc ccggcggagt
     9181 tttttgcgcc tgcattttcc aaggtttacc ctgcgctaag gggcgagatt ggagaagcaa
     9241 taagaatgcc ggttggggtt gcgatgatga cgaccacgac aactggtgtc attatttaag
     9301 ttgccgaaag aacctgagtg catttgcaac atgagtatac tagaagaatg agccaagact
     9361 tgcgagacgc gagtttgccg gtggtgcgaa caatagagcg accatgacct tgaaggtgag
     9421 acgcgcataa ccgctagagt actttgaaga ggaaacagca atagggttgc taccagtata
     9481 aatagacagg tacatacaac actggaaatg gttgtctgtt tgagtacgct ttcaattcat
     9541 ttgggtgtgc actttattat gttacaatat ggaagggaac tttacacttc tcctatgcac
     9601 atatatta
//
