LOCUS       sequence-415834-        5096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Klotzsche M, Ehrt S, Schnappinger D
  TITLE     Improved tetracycline repressors for gene silencing in mycobacteria.
  JOURNAL   Nucleic Acids Res. 2009 Apr;37(6):1778-88. doi: 10.1093/nar/gkp015. 
            Epub 2009 Jan 27.
  PUBMED    19174563
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkp015"; journalName: "Nucleic Acids Res"; date: 
            "2009-04"; volume: "37"; issue: "6"; pages: "1778-88"
COMMENT     Sequence Label: pTC-28S15-0X
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1335..1923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1415..1434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2254..3069)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2977..2996
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3061..3080
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(5016..5062)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcgacatcg atttaattaa tatgcatcaa ttgatttaaa tactagtgtt aactatttaa
       61 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcaagggg acctctaggg
      121 tccccagctg gctagcgagt catgaggtca ccatggtcat taggagccgc tctcgcactt
      181 cagctgcttc tccaagccgc agatgatcag ctccaggccg aacaagaagg ccggctcggc
      241 gccctggtgg tcgaacagct cgatggcctg gcgcagcaac ggcggcatgc tgtcggtggt
      301 cggggtctcg cgctcctcct tggcggccgt gtgctcctgg tcctccaaca cgcagcccag
      361 ggtgaagtgg cccacggccg acagggcgta caacgcgttc tccagcgaga agccgttctc
      421 ggtcatgaag cgcagctggg tctccacggt gtcgtactgc ttctcgtccg ggcgggtgcc
      481 caggtgcacc ttggcgccgt cgcggtagcg cagcagcgcg cggcggaacg acatggcgtt
      541 gttgcgcagg aacgactgcc acgactcgcc ggccgccggc agcgagtagt cgtggtggcg
      601 cgccaggatc tccaccgcca gggcgtccag cagggcccgc ttgttcttga cgtgccagta
      661 cagggtcggc tgctcgacgc ccagcttctg ggccagcttg cgggtggtca cgccctcgat
      721 gccgacctcg ttgcccaggg ccagggcgga gttgatgacc ttggacttgt ccaggcggga
      781 cattgcgaag tgattcctcc tgatggtacc gcggatcgtg ctcatttcgg gcggcgaatc
      841 tctcggcgtc gaaatgatgt atgccgtgct ggtcgtacac acgggtactc ccagagccta
      901 ctcgaccgag cacacgcagg acaaactcgc ccaccccggg tgggttctcc cgctcgtcag
      961 agacccttct cacaccgggc ctgtgcgtgc gaacgcacag atcaacgccg ggatggcgta
     1021 gagctcactg cgcagcaccg tggggatgcg gccgatcatc acgtcgcgga tcgtgccgcc
     1081 tcccacggcc gtgacggtgc cgacgatcca atatttctag tagatctgat atcctcgagg
     1141 catgccacgt gcctgcaggt ttaaacgcgg ccgcacgcgt tgcgctcggt cgttcggctg
     1201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1501 ctcccctcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1741 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1921 caagaagatc ctttgatctt ttctacgggg tctgacgctc atggaactag tgaggtctgc
     1981 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga
     2041 aagtgaggga gccacggttg gtgagagctt tgttgtaggt ggaccagttg gtgattttga
     2101 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca
     2161 actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct
     2221 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     2281 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     2341 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     2401 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     2461 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagatt
     2521 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     2581 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     2641 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     2701 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     2761 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     2821 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     2881 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatggg gcttcccata
     2941 caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     3001 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     3061 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     3121 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc
     3181 cccccccccg acgtcaggtg gctagctggc gggaacggca atgctcctgg cggcggcggg
     3241 gctggtgccc aggtctccgc acagagcggc ggtgctggcg ctcgcggcca ggcgtggttc
     3301 ttcgcgtact gacaagaaac ccccctcttt aggactcagt gtccttggga ggggggcttt
     3361 ttgcgtttca ggaggtcttg gccagcttgg acatcgcctc agcgatagcc tcgtcgcggg
     3421 cctcagacgc catctggtac ttcatcgcca tcctaggagt cgtgtgaccg agacgggcca
     3481 tcagctcctt ggtcgtcgca cctgcctgag cggcgaacgt agcgccgaca gcgcggaggt
     3541 cgtggatgcg gagttccggc cgaccgatct tggcgtagcc acgcttcagc gacttggtga
     3601 acgcggactt cgacagccgg ttgccctgcg tcgtggtcac caggaatgcc tcggggccct
     3661 tgttcatctt cgtacggtcc ttcatgtgcg ctcggatcat ctccgcgacg tgaggcggaa
     3721 ccgtcacagg acgcttcgac cggacggtct tggcgttgcc aacgacgatc ttgttcccca
     3781 cgcgggaagc gccacggcgc acccggagct tcatcgtcat gccgtcgtcc acgatgtcct
     3841 tgcggcgaag ctcgatcagc tctccgaacc ggaggctcgt ccacgccagg atgtatgccg
     3901 cgatccggta gtgcacgaag atctcagcgg cgacgatgtc cagctcctca ggcgtcagcg
     3961 cctctacgtc gcgctcatcg gctgccttct gctcgatccg gcacgggttc tctgcgatca
     4021 gcttgtcctc gaccgctgtg ttcatcaccg cccggaggac gttgtaggca tgccggcggg
     4081 cagtcgggtg cttcctaccc atcccggccc accacgcacg caccagagct ggcgtcatct
     4141 ctgtgaccgc cacttcacct agcaccgggt agatgcggcg ctccgcgtgc ccgctgtaca
     4201 gatccctggt gccgtctgcg aggtcgcgct ccacgagcca cttccgggtg tactcctcca
     4261 gcgtgatggc gctggcggct gccttcttcg cccggtcctg tggaggggtc caggtctcca
     4321 tctcgatgag ccgcttctcg cccgcgagcc aggcttcggc gtccatcttg ttgtcgtagg
     4381 tctgcagcgc gtagtacctc acaccgtcct gcgggttgac gtatgaggct tggatcctcc
     4441 cgctgcgctg agtcttcagc gatccccatc cgcgacgtgc caactaggtc tcctctcgtc
     4501 gtgaacaagg ctaccgggtt gcaactcctg tgcaactctc aggcttcaac gcgcttctac
     4561 gacctgcaat ttctttccac ttagaggatg cagccgagag ggggtaaaaa cctatcttga
     4621 ccggcccata tatgtggtcg gcagacaccc attcttccaa actagctacg cgggttcgat
     4681 tcccgtcgcc cgctccgctg gtcagagggt gttttcgccc tctggccatt tttctttcca
     4741 ggggtctgca actcttgtgc gactcttctg acctgggcat acgcggttgc aacgcatccc
     4801 tgatctggct actttcgatg ctgacaaacg aatagagccc cccgcctgcg cgaacagacg
     4861 aggggcattc acaccagatt ggagctggtg cagtgaagag aatagaccgg gacaaggttg
     4921 caccgggagt tgcagcggtc ggaaccctcg ccgtcggcgg gctggcgttc gccctgtcgt
     4981 tcacggctct agctgatcac cgcggccatg atggcataaa acgaaaggcc cagtctttcg
     5041 actgagcctt tcgttttatt tgatgcctgg cagtcgatcg tacgctagtt aactac
//
