LOCUS       sequence-41584-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-S387A
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..46
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgtcagaat tgatctacca tggactacaa agacgatgac gacaagcttg cggccgcgaa
       61 ttcaagctta gccaccatgg actacaaaga cgatgacgat aaagcaaggc tcgaatcggt
      121 acctaaggat cccgggcagc agccgccgcc tcaaccggcc ccccagggcc aagggcagcc
      181 gccttcgcag cccccgcagg ggcagggccc gccgtccgga cccgggcaac cggcacccgc
      241 ggcgacccag gcggcgccgc aggcaccccc cgccgggcat cagatcgtgc acgtccgcgg
      301 ggactcggag accgacctgg aggcgctctt caacgccgtc atgaacccca agacggccaa
      361 cgtgccccag accgtgccca tgaggctccg gaagctgccc gactccttct tcaagccgcc
      421 ggagcccaaa tcccactccc gacaggccag tactgatgca ggcactgcag gagccctgac
      481 tccacagcat gttcgagctc attcctctcc agcttctctg cagttgggag ctgtttctcc
      541 tgggacactg acccccactg gagtagtctc tggcccagca gctacaccca cagctcagca
      601 tcttcgacag tcttcttttg agatacctga tgatgtacct ctgccagcag gttgggagat
      661 ggcaaagaca tcttctggtc agagatactt cttaaatcac atcgatcaga caacaacatg
      721 gcaggacccc aggaaggcca tgctgtccca gatgaacgtc acagccccca ccagtccacc
      781 agtgcagcag aatatgatga actcggcttc aggtcctctt cctgatggat gggaacaagc
      841 catgac
//
