LOCUS       sequence-415843-        6378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrates 50xLacO repeats upstream of the BES1 promoter with a 
            blasticidin gene downstream of the promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Budzak J, Goodwin I, Tiengwe C, Rudenko G
  TITLE     Imaging of genomic loci in Trypanosoma brucei using an optimised 
            LacO-LacI system.
  JOURNAL   Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 
            10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3.
  PUBMED    37923299
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biochem
            Parasitol."; date: "2023-12"; volume: "256:111598. doi"; pages: " 
            10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3"
COMMENT     Sequence Label: pBES1-LacOx50-blast
FEATURES             Location/Qualifiers
     source          1..6378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(412..1000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(492..511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1171..1962,1963..2031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1794..1813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2032..2136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2204..2222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2260..2282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2382..2401
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3572..3970)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    4583..4599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4632..4648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4663..4679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4741..4757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4772..4788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4852..4868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4883..4899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4962..4978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5023..5039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5072..5088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5103..5119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5182..5198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5213..5229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5244..5260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5292..5308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5323..5339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5354..5370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5403..5419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5434..5450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5465..5481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5514..5530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5616..5639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5651..5667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5682..5698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5731..5747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5792..5808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5841..5857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5872..5888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5951..5967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5982..5998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6013..6029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6062..6078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6093..6109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6232..6248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    6311..6327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agcgcaagac cttcatttat cacgtgtttc tcgtgtgaaa agaacgcctc ataactctta
       61 aaaataaaca aatgggaaat aaggtatcat atcaaagctg cgacaaatgt attatccagt
      121 gtcttttctt agtaaaaaac cgaaatatcg ctcagcatta tagtgattct aaccctgtga
      181 aaagtactaa agaaggcttt agtgatatgc acgtcgatat tccttacctc atacccataa
      241 aggaagaatc accaacttta cgattgctta cttcactata aattggagcc tagcatctgt
      301 aaaaacttac ctatttaaca tcaaggttag tcgtcagacc taggttcaag acatgtgagc
      361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2161 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2221 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     2281 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     2341 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     2401 actgagagtg caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     2461 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcaagg agatggcgcc
     2521 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctgtacta
     2581 ggtacctcct gcgtcgttcc cacgcatatc aaccatcaat aattttcagt atatttgtac
     2641 ggattcccga gcacagccac cgtctcacga cgattttact gtcttgcttc ctcctcccca
     2701 gcacccctcg cgggcacttg tcaaacacct cagcactctg cgttattacc ccacccacac
     2761 cgtttgggcc atttacacac gctctctaca cgcttcaagc cttcgccatc ctcgcgcacg
     2821 atgtccagtt accatttccc tcttcacgtt tttcctaacc ctcacaatct ccgatcatgc
     2881 taattccacc atttcctcta atcctttcac tcttccgttg cacccctgct cttcgtaccg
     2941 gtcgaaaaat taagattcaa aaccacggaa aataaaaaaa attaaaaaat ttaaaaaaat
     3001 tctggttgaa ggacgcggta aaaaaatttt taaaaatgga ggtgcgcggg cggggggggg
     3061 gaggagggtt agggtccgca taacaaaaca gccgctgcat ggttgacaaa aaaattgaaa
     3121 acacaataaa aaattaaaca ttggcgcaaa aaaaaaaaaa ggagccgcga atggctaaaa
     3181 aaaaaaatta aaaatccagg atagaggtgt gagttaaaaa aaaagaggaa aaaattaacc
     3241 ctaatcctaa tcctaatcct aatcctaagc ctaagtctaa tcctaatcct aagcctaacc
     3301 cccctaacta accccctaac taacctaact aacctaccta actacccccc cccccattcc
     3361 tacctaccta caaaaaaaac aggaaaaaag aaaaaaattt tagttgccag aaaatatttc
     3421 tagacaatca gacagtcaac ttacaaaaca cagaacacaa aatctcggct ggttttggtg
     3481 ttaaaatata tcaaaaagtt ttcaagtagc aatgaaaatt ttaaaaggag gggggaaatt
     3541 tgagggggga aagggctgca ggaatttcga gttagccctc ccacacataa ccagagggca
     3601 gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga
     3661 tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc
     3721 tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag
     3781 tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag
     3841 tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg
     3901 ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag
     3961 gcttggccat agcttatttt atggcagcaa cgagacctta cgtaaagcaa aactaaataa
     4021 gagcggagac tgcaatgcag agtaaaaaaa aaaaaaacat gaatttcaga agaccttgct
     4081 gtgcccgggc tctgagagcg gtcagttgct ctcctgctcc gtacgcggtc tctttgcatc
     4141 gcacgcaatc gtcacagtgg taacgctaag ggttttcgca gccacacagt cgacataaat
     4201 cgaatacggg cactccggta acgacggcac gctcaggcca actcacgatc ggttcgagac
     4261 gtccgccaca ccttgagtct gatatcctag atttctgtaa taacctcccc tgtaatataa
     4321 actggtgtgg taatagggat atgattcttt tagaaggtta ttccgcttgg atggccattg
     4381 agtcggcgtt ctttcagact cgttttttgt aaaacatggg acaacgttaa tgcaactcct
     4441 ccgaatacat ctccctttat ttaaagaaaa aaatcccacc ctctctctct ccgagagcac
     4501 agttgctagc aagcggacct taattgtggc ggataacaat taaataatag gaattgtgag
     4561 cgatagcaat tagcgtagca aattgtgagc ggataacaat tggagcaaga gctctagcat
     4621 ggagaaacga attgtgagcg gataacaatt tacgggtcag aattgtgagc ggataacaat
     4681 tacggacgga aaattgtgag cgataacaat tgaagaagta actctagcag gaagggacaa
     4741 ttgtgagcgg ataacaatta agcgattcaa attgtgagcg gataacaatt aagggtagaa
     4801 aattgtgagc ggatagcaat ttgaacgaac actctagcac cgactcgaaa attgtgagcg
     4861 gataacaatt agaaaagccg aattgtgagc ggataacaat tagctactaa gaattggagc
     4921 ggataacaat tgaaccagga actctagcaa gcaagcaata attgtgagcg gataacaatt
     4981 aggagagaga aattgtggcg gataacaatt ataataaaga aattgtgagc ggataacaat
     5041 taaaaagaaa actctagcag caaaaggcaa attgtgagcg gataacaatt cagtctacca
     5101 aattgtgagc ggataacaat tcgacgtcta aaattatgag cggataacaa ttgacacagg
     5161 agctctagca tgaaaggaaa attgtgagcg gataacaatt agatcaaagc aattgtgagc
     5221 ggataacaat acaaagaaaa aaattgtgag cggataacaa ttaagtagga agctctagca
     5281 gaccggaaga attgtgagcg gataacaatt gtgtgagaaa aattgtgagc ggataacaat
     5341 tcgctaaggg caattgtgag cggataacaa ttagtaattg gactctagca cataggacga
     5401 aattgtgagc ggataacaat tgaaaggtgc caattgtgag cggataacaa ttggtacatt
     5461 ggaattgtga gcggataaca attgagacac atgctctagc aactagagac aaattgtgag
     5521 cggataacaa ttgaaagaat tgtgagcgga taactattgg gtcgcggaaa ttgtgaacgg
     5581 ataacaatta atagacaagc tctagcagag ataggggaat tgtgagcgga taacaattca
     5641 taaccataaa ttgtgagcgg ataacaattc cagacgccaa attgtgagcg gataacaatc
     5701 agtagtgacg ctctagcaaa aaaaatacaa ttgtgagcgg ataacaatta gacaggggaa
     5761 attgtgagcg gatacaatta aaggaaggaa attgtgagcg gataacaatt ctcatagcgg
     5821 ctctagcatg ttagcattaa ttgtgagcgg ataacaatta ccgcgggtta attgtgagcg
     5881 gataacaatt aggcgaagag aattgtgacg gataacaatt ataaaaggga ctctagcagg
     5941 cagtaataaa ttgtgagcgg ataacaattc ctcccatgaa attgtgagcg gataacaatt
     6001 cagacccgta aattgtgagc ggataacaat taaagcggga actctagcag gggggatgaa
     6061 attgtgagcg gataacaatt aacgatggaa aattgtgagc ggataacaat taggggtggg
     6121 caattgtgag cggatcaatt tgaagagtag ctctagcaag gtaaagacaa ttgtgagcgg
     6181 ataaaattca ggaaactaaa ttgtgatcgg ataacaattg cattaggcta attgtgagcg
     6241 gataacaatt gccacagtaa ctctagcaaa cgaggcggaa ttgcgagcgg taacaattgc
     6301 aaagtcatca ttgtgagcgg ataacaattg aaaaacccac tctagcaaga cgtcgatcat
     6361 aagcttaaca attaagac
//
