LOCUS       sequence-415845-        8821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2064
FEATURES             Location/Qualifiers
     source          1..8821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1759..2347
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1812..1832)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2230..2241)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2419..2652
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2730..2851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2767..2786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2821..2840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2891..3026
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3046..3065)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3047..3065)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3075..3092)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3075..3091)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3084..3106)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3233..3688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3320..3339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3530..3551
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3714..3818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3819..3887,3888..4679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4037..4056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4850..5438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5339..5358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5592..5609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5726..5747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5762..5767,5768..5785,5786..5792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5800..5816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5805..5827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5824..5840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5824..5840
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5859..5879
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5861..5879
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5907..6133
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6134..6314
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6361..6486
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6979..7212
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7397..7441
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7672..7882
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7694..7890
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7741..7876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7922..8524
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7922..7941)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8418..8438
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8567..8684
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 ccccagccct gctctcacgc ccacgccctt ctcagtcaaa gacatcctaa acctggaaca
     1261 gcagcagcgc agcctggctg ccgccggaga gctctctgcc cgcctggagg cgaccctggc
     1321 gccctcctcc tgcatgctgg ccgccttcaa gccagaggcc tacgctgggc ccgaggcggc
     1381 tgcgccgggc ctcccagagc tgcgcgcaga gctgggccgc gcgccttcac cggccaagtg
     1441 tgcgtctgcc tttcccgccg cccccgcctt ctatccacgt gcctacagcg accccgaccc
     1501 agccaaggac cctagagccg aaaagaaagg gtgtgaactg ccccgagggc agagacctcc
     1561 cgttttgttc tccagcgcct tgagccagcc tgactttcta caaatgctga gtgagacgtg
     1621 tcggtggctc ccagtgcact tggcagagta gtaatgaact agtccgtaac ttgaaagtat
     1681 ttcgatttct tggctttata tatcttgtgg aaaggacgac tgggtggata aacgtcgcgt
     1741 ggtacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     1801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     1861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     1921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     1981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2161 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2281 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2341 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2401 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     2461 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2641 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     2701 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     2761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     2881 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2941 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3001 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3061 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3241 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3301 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3361 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3421 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3481 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3541 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3601 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3661 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     3721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5821 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     5881 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     5941 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6001 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6061 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6121 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6181 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6241 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6301 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6361 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6421 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6481 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6541 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6601 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     6661 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     6721 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     6781 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     6841 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6901 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6961 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7021 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7081 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7141 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7201 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7261 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7321 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7381 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7441 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7501 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7561 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     7621 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7681 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     7741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7801 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7861 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     7921 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7981 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     8041 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8101 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     8161 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8221 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8281 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8341 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8401 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8461 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     8521 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     8581 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     8641 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     8701 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
     8761 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     8821 t
//
