LOCUS       sequence-41585-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Rishi V, Gal J, Krylov D, Fridriksson J, Boysen MS, Mandrup S, 
            Vinson C
  TITLE     SREBP-1 dimerization specificity maps to both the helix-loop-helix 
            and leucine zipper domains: use of a dominant negative.
  JOURNAL   J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31.
  PUBMED    14702347
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-19"; volume: "279(12)"; pages: "11863-74. 
            Epub 2003 Dec 31"
COMMENT     Sequence Label: CMV500 A-SREBP2
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             113..145
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(445..463)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(446..463)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(483..500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    489..713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcca ccatggacta caaggacgac gatgacaagc atatggctag
      121 catgactggt ggacagcaaa tgggtcggga tcccgacgaa gaggaagatg acgaagaaga
      181 actcgaggaa ctggaagaca gcatcataga gttgaaggac ttggtcatgg gaacagatgc
      241 caagatgcac aaatctggcg ttctgaggaa ggccattgat tacatcaaat atttgcagca
      301 ggtcaatcac aagcttcgtc aggagaacat ggtactgaag ctggcaaatc agaaaaacta
      361 agaattcaag cttcgtcagg agaacatggt actgaagctg gcaaatcaga aaaactaaga
      421 attcaagctt ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
      481 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      541 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      601 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
      661 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
      721 ggcggaaaga accagctggg gctcgagggg ggatccccac gcgccctgta gcggcgcatt
      781 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccc
//
