LOCUS       sequence-415854-       11416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2626
FEATURES             Location/Qualifiers
     source          1..11416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4354..4942
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4407..4427)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4825..4836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5014..5247
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5325..5446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5362..5381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5416..5435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5486..5621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5641..5660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5642..5660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5670..5687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5670..5686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5679..5701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5828..6283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5915..5934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6125..6146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6309..6413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6414..6482,6483..7274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6632..6651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7445..8033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7934..7953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8187..8204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8321..8342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8357..8362,8363..8380,8381..8387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8395..8411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8400..8422
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8419..8435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8419..8435
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8454..8474
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8456..8474
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8502..8728
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8729..8909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8956..9081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9574..9807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9992..10036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10267..10477
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10289..10485
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10336..10471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10517..11119
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10517..10536)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11013..11033
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11162..11279
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggt
     1201 gctgcccacc tgccccatgg cggagttcgc gctgccgcgg cacagcgcgg tcatggagcg
     1261 ccttcgccgg cgcatcgagc tgtgccggcg ccaccacagc acctgcgagg cccgctacga
     1321 ggccgtgtcg cccgagcgcc tggagctgga gcgccaacac accttcgccc tgcaccagcg
     1381 ctgcatccag gccaaggcca agcgcgccgg gaagcacagg cagccgcccg ccgccacggc
     1441 cccggcgccc gccgccccgg ccccgcgcct ggacgccgct gacggccccg agcacggccg
     1501 cccggccacg catcttcatg atacagttaa gaggaatctt gacagcgcca cttcccctca
     1561 gaatggcgat caacagaatg gctacgggga cctctttcct gggcataaga agactcgccg
     1621 ggaggcccct ctgggagttg ccatctcttc caatggactg cctccagcct cccccctcgg
     1681 tcagtctgac aagccttctg gagccgacgc cctgcagtcc agtgggaagc actctctggg
     1741 gctagactct ctcaacaaaa agcgtctggc tgactccagc cttcacttga atggaggcag
     1801 taaccccagt gagtcatttc ctctgagcct gaataaagaa ctgaagcagg agcctgtcga
     1861 agacctgcct tgcatgatca ctgggactgt cggctccata tcgcaaagca acctcatgcc
     1921 agacctcaac cttaacgagc aggagtggaa ggagctcatc gaggagctga acaggtcggt
     1981 gcccgatgaa gacatgaagg acctgtttaa tgaggacttc gaggagaaga aggacccaga
     2041 gtcttctggc tctgccacac aaaccccctt ggcacaggac attaatatta agacggaatt
     2101 ctctccagca gcctttgagc aagaacagtt aggctctcca caagtgaggg ccgggtctgc
     2161 agggcagacc tttctggggc cttcctctgc ccctgtgagt acagattccc ccagcctagg
     2221 gggctcccaa accttattcc acacctctgg tcagccccgg gcggacaatc ccagtccaaa
     2281 cctgatgccg gcatcagccc aggcccagaa cgcacaaaga gcccttgcag gtgtggtatt
     2341 gcccagtcag ggcccaggag gggcctcaga gctgtcctct gcccaccagc tccagcagat
     2401 cgctgccaag cagaagcgcg agcagatgct ccagaaccca cagcaggcca ccccggcacc
     2461 agccccgggc cagatgtcca catggcagca gacggggccc tcccacagtt ccttagatgt
     2521 cccttacccc atggagaagc ctgccagccc ttccagctac aagcaagact tcactaactc
     2581 caaactgctc atgatgccta gtgtgaataa gagttcccct cggcccggag gcccctacct
     2641 ccagcccagc catgtgaacc tgctgagtca ccagccaccg agtaacttga atcagaactc
     2701 cgcgaataac caggggtctg tgctggacta cggcaataca aaaccccttt ctcattacaa
     2761 agcggactgt gggcaaggca gcccggggtc tggccagagc aagccagccc tgatggctta
     2821 tcttccccag cagctgtccc atataagtca cgagcagaac tccctgtttc tgatgaagcc
     2881 aaagccagga aatatgcctt tccgatcact ggttccacct ggccaggagc agaacccttc
     2941 cagtgtccct gtgcaagccc aggctaccag tgttgggacc cagccgcctg ccgtgtccgt
     3001 ggccagctcc cacaacagct ccccctatct cagcagccag caacaggccg ctgtaatgaa
     3061 gcagcatcag ttgcttttgg accaacagaa acaaagggag cagcagcaaa agcatttaca
     3121 gcaacagcag ttccttcaga ggcaacagca ccttctcgcg gaacaggaga agcaacagtt
     3181 tcagcgccat ctgacccgcc caccacccca gtaccaagac ccgacacaag gcagcttccc
     3241 acagcaggtt ggacagttca cagggtcctc tgctgccgtg cccggcatga acaccttggg
     3301 tccatccaac tccagctgtc ctcgagtgtt ccctcaggct gggaatctga tgccaatggg
     3361 ccctggacat gcttcagttt cctctctccc cacaaactca ggccaacagg accggggtgt
     3421 ggctcagttc cctggctccc aaaacatgcc tcagagcagc ctctatggca tggcttctgg
     3481 cataacccag atagttgccc agcccccgcc acaggccacc aatggacatg cccacattcc
     3541 acggcagacc aacgtgggcc agaacacctc cgtctcagct gcctatgggc agaactctct
     3601 gggaagctct ggcctctccc agcagcacaa taaggggacc ctgaaccctg gtttaacaaa
     3661 gccaccggtc ccaagggtgt caccagccat gggaggccag aattcctcct ggcagcatca
     3721 gggaatgccg aacctcagtg gccagacccc agggaacagc aacgtgagtc ccttcactgc
     3781 agcctccagt ttccacatgc agcagcaggc ccacctgaaa atgtctagcc cgcaattctc
     3841 ccaggcagtg cccaacaggc ccatggctcc catgagctca gcagctgccg tggggtcctt
     3901 gctaccccca gtgagtgcac agcagaggac cagcgcccct gccccagcac cacccccaac
     3961 agcccctcag cagggcttgc ctggcctgag cccagcaggg cctgagctgg gggccttcag
     4021 ccagagccct gcctcacaga tgggcggtcg ggcggggctg cactgcaccc aggcctaccc
     4081 tgtgcggacc gcgggccagg agctgccttt tgcctatagc gggcagccag gtggcagtgg
     4141 gctctctagt gtggctggac acaccgatct gatcgactcc ctgctgaaga acaggacttc
     4201 agaggagtgg atgagtgatt tggacgacct gttagggtct cagtagtaat gaactagtcc
     4261 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaggacga
     4321 catcttactt aatctgtgac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     4381 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4441 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4501 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4561 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4621 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4681 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4741 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4801 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4861 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4921 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     4981 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5041 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5101 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5161 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5221 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     5281 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5461 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5521 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5581 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5641 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5701 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5761 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5821 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5881 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5941 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6001 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6061 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6121 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6181 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6241 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7561 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8461 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     8521 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     8581 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     8641 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     8701 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     8761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8881 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     8941 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9001 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9061 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     9121 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     9181 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     9241 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     9301 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     9361 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     9421 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     9481 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     9541 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     9601 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     9661 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     9721 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     9781 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     9841 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9901 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9961 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10021 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10081 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    10141 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    10201 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    10261 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
    10321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    10441 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
    10501 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
    10561 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
    10621 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
    10681 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
    10741 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
    10801 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
    10861 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
    10921 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
    10981 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
    11041 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
    11101 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
    11161 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    11221 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    11281 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
    11341 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
    11401 gaaaggagtg ggaatt
//
