LOCUS       sequence-415857-       11362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0863
FEATURES             Location/Qualifiers
     source          1..11362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4300..4888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4353..4373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4771..4782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4960..5193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5271..5392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5308..5327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5362..5381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5432..5567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5587..5606)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5588..5606)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5616..5633)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5616..5632)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5625..5647)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5774..6229
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5861..5880)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6071..6092
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6255..6359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6360..6428,6429..7220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6578..6597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7391..7979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7880..7899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8133..8150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8267..8288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8303..8308,8309..8326,8327..8333)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8341..8357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8346..8368
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8365..8381
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8365..8381
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8400..8420
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8402..8420
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8448..8674
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8675..8855
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8902..9027
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9520..9753
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9938..9982
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10213..10423
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10235..10431
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10282..10417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10463..11065
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10463..10482)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10959..10979
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11108..11225
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ccaagttgca atgtccaccc tccccgttga agatgaggag tcctcggaga gcaggatggt
     1261 ggtgacattc ctcatgtcag ctctcgagtc catgtgtaaa gaactggcca agtccaaagc
     1321 cgaagtggcc tgcattgcag tgtatgaaac agacgtgttt gtcgtcggaa ctgaaagagg
     1381 acgtgctttt gtcaatacca gaaaggattt tcaaaaagat tttgtaaaat attgtgttga
     1441 agaagaagaa aaagctgcag agatgcataa aatgaaatct acaacccagg caaatcggat
     1501 gagtgtagat gctgtagaaa ttgaaacact cagaaaaaca gttgaggact atttctgctt
     1561 ttgctatggg aaagctttag gcaaatccac agtggtacct gtaccatatg agaagatgct
     1621 gcgagaccag tcggctgtgg tagtgcaggg gcttccggaa ggtgttgcct ttaaacaccc
     1681 cgagaactat gatcttgcaa ccctgaaatg gattttggag aacaaagcag ggatttcatt
     1741 catcattaag agaccttttt tagagccaaa gaagcatgta ggtggtcgtg tgatggtaac
     1801 agatgctgac aggtcaatac tatctccagg tggaagttgt ggccccatca aagtgaaaac
     1861 tgaacccaca gaagattctg gcatttccct ggaaatggca gctgtgacag taaaggaaga
     1921 atcagaagat cctgattatt atcaatataa cattcaagca ggcccttctg aaactgatga
     1981 tgttgatgaa aaacagcccc tatcgaagcc tttgcaagga agccaccatt cttcagaggg
     2041 caatgaaggc acagaaatgg aagtaccagc agaagattct actcaacatg tcccttcaga
     2101 aacaagtgag gaccctgaag ttgaggtgac tattgaagat gatgattatt ctccaccgtc
     2161 taagagacca aaggccaatg agctaccgca gccaccagtc ccggaacccg ccaatgctgg
     2221 gaagcggaaa gtgagggagt tcaacttcga gaaatggaat gctcgcatca ctgatctacg
     2281 taaacaagtt gaagaattgt ttgaaaggaa atatgctcaa gccataaaag ccaaaggtcc
     2341 ggtgacgatc ccgtaccctc ttttccagtc tcatgttgaa gatctttatg tagaaggact
     2401 tcctgaagga attcctttta gaaggccatc tacttacgga attcctcgcc tggagaggat
     2461 attacttgca aaggaaagga ttcgttttgt gattaagaaa catgagcttc tgaattcaac
     2521 acgtgaagat ttacagcttg ataagccagc ttcaggagta aaggaagaat ggtatgccag
     2581 aatcactaaa ttaagaaaga tggtggatca gcttttctgc aaaaaatttg cggaagcctt
     2641 ggggagcact gaagccaagg ctgtaccgta ccaaaaattt gaggcacacc cgaatgatct
     2701 gtacgtggaa ggactgccag aaaacattcc tttccgaagt ccctcatggt atggaatccc
     2761 aaggctggaa aaaatcattc aagtgggcaa tcgaattaaa tttgttatta aaagaccaga
     2821 acttctgact cacagtacca ctgaagttac tcagccaaga acgaatacac cagtcaaaga
     2881 agattggaat gtcagaatta ccaagctacg gaagcaagtg gaagagattt ttaatttgaa
     2941 atttgctcaa gctcttggac tcaccgaggc agtaaaagta ccatatcctg tgtttgaatc
     3001 aaacccggag ttcttgtatg tggaaggctt gccagagggg attcccttcc gaagccctac
     3061 ctggtttgga attccacgac ttgaaaggat cgtccgcggg agtaataaaa tcaagttcgt
     3121 tgttaaaaaa cctgaactag ttatttccta cttgcctcct gggatggcta gtaaaataaa
     3181 cactaaagct ttgcagtccc ccaaaagacc acgaagtcct gggagtaatt caaaggttcc
     3241 tgaaattgag gtcaccgtgg aaggccctaa taacaacaat cctcaaacct cagctgttcg
     3301 aaccccgacc cagactaacg gttctaacgt tcccttcaag ccacgaggga gagagttttc
     3361 ctttgaggcc tggaatgcca aaatcacgga cctaaaacag aaagttgaaa atctcttcaa
     3421 tgagaaatgt ggggaagctc ttggccttaa acaagctgtg aaggtgccgt tcgcgttatt
     3481 tgagtctttc ccggaagact tttatgtgga aggcttacct gagggtgtgc cattccgaag
     3541 accatcgact tttggcattc cgaggctgga gaagatactc agaaacaaag ccaaaattaa
     3601 gttcatcatt aaaaagcccg aaatgtttga gacggcgatt aaggagagca cctcctctaa
     3661 gagccctccc agaaaaataa attcatcacc caatgttaat actactgcat caggtgttga
     3721 agaccttaac atcattcagg tgacaattcc agatgatgat aatgaaagac tctcgaaagt
     3781 tgaaaaagct agacagctaa gagaacaagt gaatgacctc tttagtcgga aatttggtga
     3841 agctattggt atgggttttc ctgtgaaagt tccctacagg aaaatcacaa ttaaccctgg
     3901 ctgtgtggta gttgatggca tgcccccggg ggtgtccttc aaagccccca gctacctgga
     3961 aatcagctcc atgagaagga tcttagactc tgccgagttt atcaaattca cggtcattag
     4021 accatttcca ggacttgtga ttaataacca gctggttgat cagagtgagt cagaaggccc
     4081 cgtgatacaa gaatcagctg aaccaagcca gttggaagtt ccagccacag aagaaataaa
     4141 agagactgat ggaagctctc agatcaagca agaaccagac cccacgtggt agtaatgaac
     4201 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     4261 atgcgtcatg gatccgtaac tcttacgcgt taagtcgaca atcaacctct ggattacaaa
     4321 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4381 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4441 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4501 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4561 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4621 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4681 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4741 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4801 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4861 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     4921 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4981 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5041 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5101 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5161 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     5221 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     5281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5401 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5461 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5521 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     5581 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     5641 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5701 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5761 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6121 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6181 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     6241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8161 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8281 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8341 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     8401 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     8461 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     8521 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     8581 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8641 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8821 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8881 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8941 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     9001 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     9061 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     9121 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     9181 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     9241 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     9301 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9361 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9421 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9481 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9541 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9601 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9661 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9721 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9781 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9841 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9901 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9961 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    10021 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    10081 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    10141 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
    10201 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
    10261 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10321 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10381 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
    10441 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    10501 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    10561 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
    10621 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
    10681 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    10741 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    10801 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    10861 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    10921 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    10981 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    11041 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
    11101 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    11161 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    11221 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    11281 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    11341 ggtcttgaaa ggagtgggaa tt
//
