LOCUS       sequence-41587-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Qyang Y, Luo X, Lu T, Ismail PM, Krylov D, Vinson C, Sawadogo M
  TITLE     Cell-type-dependent activity of the ubiquitous transcription factor 
            USF in cellular proliferation and transcriptional activation.
  JOURNAL   Mol Cell Biol. 1999 Feb;19(2):1508-17.
  PUBMED    9891084
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1999-02"; volume: "19(2)"; pages: "1508-17"
COMMENT     Sequence Label: CMV566 A-USF
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             92..118
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             122..154
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(520..538)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(521..538)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(558..575)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    564..675
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ccaccatggc gtatccctac gacgtgcccg attatgccca
      121 tatggctagc atgactggtg gacagcaaat gggtcgggat cccgacgaag aggaagatga
      181 cgaagaagaa ctcgaggaag acctggaaaa ctggatcgtg cagctctcca agataatccc
      241 agactgctct atggagagca ccaagtctgg ccagagtaaa ggtgggattc tatccaaagc
      301 ttgtgattat atccaggagc ttcggcagag taaccaccgc ttgtctgaag aactgcaggg
      361 acttgaccaa ctgcagctgg acaatgacgt gcttcgacaa caggtggaag atcttaaaaa
      421 caagaatctg ctgcttcgag ctcagttgcg gcaccacgga ttagaggtcg tcatcaagaa
      481 tgacagcaac taagaattca agcttctaga gggccctatt ctatagtgtc acctaaatgc
      541 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
      601 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
      661 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggg
//
