LOCUS       sequence-415880-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OmpT_native membrane expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Rath P, Hermann A, Schaefer R, Agustoni E, Vonach JM, Siegrist M, 
            Miscenic C, Tschumi A, Roth D, Bieniossek C, Hiller S
  TITLE     High-throughput screening of BAM inhibitors in native membrane 
            environment.
  JOURNAL   Nat Commun. 2023 Sep 13;14(1):5648. doi: 10.1038/s41467-023-41445-w.
  PUBMED    37704632
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-13"; volume: "14(1):5648. doi"; pages: " 
            10.1038/s41467-023-41445-w"
COMMENT     Sequence Label: OmpT
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             103..123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgaaaaagt tattaccctg taccgcactg gtgatgtgtg cgggaatggc ctgcgcacag
       61 gccatgggca gcagccatca tcatcatcat cacagcagcg gcgaaaacct gtacttccag
      121 ggatcctcta ccgagacttt atcgtttact cctgacaaca taaatgcgga cattagtctt
      181 ggaactctga gcggaaaaac aaaagagcgt gtttatctag ccgaagaagg aggccgaaaa
      241 gtcagtcaac tcgactggaa attcaataac gctgcaatta ttaaaggtgc aattaattgg
      301 gatttgatgc cccagatatc tatcggggct gctggctgga caactctcgg cagccgaggt
      361 ggcaatatgg tcgatcagga ctggatggat tccagtaacc ccggaacctg gacggatgaa
      421 agtagacacc ctgatacaca actcaattat gccaacgaat ttgatctgaa tatcaaaggc
      481 tggctcctca acgaacccaa ttaccgcctg ggactcatgg ccggatatca ggaaagccgt
      541 tatagcttta cagccagagg tggttcctat atctacagtt ctgaggaggg attcagagat
      601 gatatcggct ccttcccgaa tggagaaaga gcaatcggct acaaacaacg ttttaaaatg
      661 ccctacattg gcttgactgg aagttatcgt tatgaagatt ttgaactcgg tggcacattt
      721 aaatacagcg gctgggtgga atcatctgat aacgatgaac actatgaccc gggaaaaaga
      781 atcacttatc gcagtaaggt caaagaccaa aattactatt ctgttgcagt caatgcaggt
      841 tattacgtca cacctaacgc aaaagtttat gttgaaggcg catggaatcg ggttacgaat
      901 aaaaaaggta atacttcact ttatgatcac aataataaca cttcagacta cagcaaaaat
      961 ggagcaggta tagaaaacta taacttcatc actactgctg gtcttaagta cacattttaa
     1021 caagctt
//
