LOCUS       sequence-415882-         489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses chaperone protein SKP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Rath P, Hermann A, Schaefer R, Agustoni E, Vonach JM, Siegrist M, 
            Miscenic C, Tschumi A, Roth D, Bieniossek C, Hiller S
  TITLE     High-throughput screening of BAM inhibitors in native membrane 
            environment.
  JOURNAL   Nat Commun. 2023 Sep 13;14(1):5648. doi: 10.1038/s41467-023-41445-w.
  PUBMED    37704632
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-13"; volume: "14(1):5648. doi"; pages: " 
            10.1038/s41467-023-41445-w"
COMMENT     Sequence Label: Skp
FEATURES             Location/Qualifiers
     source          1..489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcgagaacc tatatttcca gggcagccat
       61 atggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt agcgcagaaa
      121 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
      181 atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat
      241 cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
      301 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
      361 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
      421 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
      481 gttaaataa
//
