LOCUS       sequence-415886-        9663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Li QJ, Chau J, Ebert PJ, Sylvester G, Min H, Liu G, Braich R, 
            Manoharan M, Soutschek J, Skare P, Klein LO, Davis MM, Chen CZ
  TITLE     miR-181a is an intrinsic modulator of T cell sensitivity and 
            selection.
  JOURNAL   Cell. 2007 Apr 6. 129(1):147-61.
  PUBMED    17382377
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Apr 6. 129(1):147-61."
COMMENT     Sequence Label: MDH1-miR-181a1-SHP2
FEATURES             Location/Qualifiers
     source          1..9663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1421..1908
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     1841..1860
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3799..3808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3805..3807,3808..3810,3811..4524)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3850..3871)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4111..4130)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4458..4479
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4603..5064
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4659..4676)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4886..4905
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        complement(5986..6076)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(6046..6065)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    6732..6748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6756..6762,6763..6780,6781..6786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6801..6822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6939..6956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7110..7698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7190..7209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7869..8660,8661..8729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8492..8511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8730..8834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8902..8920
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8958..8980)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9080..9099
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9293..9315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9443..9462
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9662..9663,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatccctacc gggtagggga ggcgcttttc
     1441 ccaaggcagt ctggagcatg cgctttagcg gccccgctag gcacttggcg ctacacaagt
     1501 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     1561 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     1621 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     1681 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     1741 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccag gggcgggctc
     1801 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccga cattctgcac gcttcaaaag
     1861 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcactcga
     1921 agcggccgcg ttaacgtcga cagatctacg cgtgccacca tgacatcgcg gagatggttt
     1981 caccccaaca tcactggtgt ggaggcagag aatctcctgc tgaccagagg agtcgatggc
     2041 agttttttag caaggcccag taagagtaac cctggagact tcactctgtc tgttagaaga
     2101 aatggagctg ttacccacat caagattcag aacactgggg actactatga cctctatggt
     2161 ggggagaagt ttgccacttt ggctgaactg gttcagtatt acatggaaca ccatgggcag
     2221 ctgaaagaga agaatggaga tgttatcgag ctcaagtacc cgctgaactg tgcagaccct
     2281 acctctgaaa ggtggttcca tggtcacttg tctggaaaag aagcagagaa gctgctgacg
     2341 gagaagggca agcatggcag cttcctcgtt cgagagagcc agagccaccc cggagacttc
     2401 gttctctccg tgcgcactgg tgacgacaaa ggggagagca acgacggcaa gtccaaagtg
     2461 acccacgtca tgatccgctg tcaggagctg aaatacgacg ttggtggggg agagcgcttt
     2521 gactctctga cagacctggt ggagcattac aagaagaacc ccatggtgga gacgctgggc
     2581 acagtcctgc agctcaagca gcccctcaac acaactcgta tcaatgctgc tgaaattgaa
     2641 agccgggttc gagagttaag caagctggct gagaccacag ataaagtcaa gcagggcttt
     2701 tgggaagagt ttgagacgct ccagcaacag gaatgcaaac ttctctatag ccgaaaagaa
     2761 ggacagagac aagaaaataa aaacaaaaac agatacaaaa acatcctgcc ctttgatcat
     2821 accagggtcg ttctgcatga tggggatccc aatgagcctg tttctgatta cattaatgca
     2881 aacatcatca tgcctgagtt tgagaccaag tgcaacgatt ccaaacccaa aaagagttac
     2941 attgccactc aaggctgcct gcagaacacg gtgaatgact tctggcggat ggtgttccag
     3001 gagaactctc gagtcattgt catgaccaca aaggaagtgg agagagggaa gagcaaatgt
     3061 gtcaagtact ggcctgatga gtatgcgctc aaagaatacg gggtcatgcg tgttaggaac
     3121 gtcaaagaaa gtgccgccca tgactacact ttatgagagc tcaaactctc taaggtcgga
     3181 caagctctac tccagggaaa cacagagaga accgcctggc agtaccactt tcggacctgg
     3241 ccagaccatg gcgtgcctag tgaccctgga ggtgtgctgg acttcctgga ggaggtccac
     3301 cacaagcagg agagcatcgt ggatgcaggc cctgtcgtgg ttcactgcag cgctgggatt
     3361 ggccggacag gaaccttcat tgtgattgac atccttattg acatcattcg agagaaaggc
     3421 gtggactgtg acatcgacgt tcctaaaacc attcagatgg tgcggtccca gaggtcgggg
     3481 atggtccaga cagaagcaca gtaccggttt atctacatgg ctgtccagca ctacatagag
     3541 acgctgcagc gccggatcga ggaggagcag aaaagcaaaa gaaaaggaca tgaatatacc
     3601 aatattaagt attccttggt ggaccagaca agtggtgatc agagtcccct gccaccctgc
     3661 accccaacgc caccctgtgc agaaatgagg gaggacagcg cccgagtcta tgagaacgtg
     3721 ggcctcatgc agcagcagag gagtttcaga ggggccgcag gtggaggcgg atcaggtggc
     3781 ggtggaagtg gaccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3841 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3901 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3961 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4021 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4081 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4141 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4201 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4261 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4321 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4381 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
     4441 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4501 ggcatggacg agctgtacaa gtaatgagga tcaccgggca attgaagatc tagtcgacca
     4561 attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4621 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4681 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4741 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4801 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4861 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4921 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4981 tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5041 ttcctttgaa aaacacgatg ataataccat gaaaacattt aacatttctc aacaggatct
     5101 agaattagta gaagtagcga cagagaagat tacaatgctt tatgaggata ataaacatca
     5161 tgtgggagcg gcaattcgta cgaaaacagg agaaatcatt tcggcagtac atattgaagc
     5221 gtatatagga cgagtaactg tttgtgcaga agccattgcg attggtagtg cagtttcgaa
     5281 tggacaaaag gattttgaca cgattgtagc tgttagacac ccttattctg acgaagtaga
     5341 tagaagtatt cgagtggtaa gtccttgtgg tatgtgtagg gagttgattt cagactatgc
     5401 accagattgt tttgtgttaa tagaaatgaa tggcaagtta gtcaaaacta cgattgaaga
     5461 actcattcca ctcaaatata cccgaaatta atgagttaac ttaattaaga attgcggccg
     5521 cgtcgacctg cagccaagct tatcgataaa ataaaagatt ttatttagtc tccagaaaaa
     5581 ggggggaatg aaagacccca cctgtaggtt tggcaagaat tcgttttatc gataaaataa
     5641 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     5701 aagaattcta ggaattctta attaaaaaag aatcctgtcg ggtcctgctt tgcggttctg
     5761 tcttcagcaa acgcagaggg ccatggagta gatgatggtt agctataggg tacaatcaac
     5821 ggtcgatggt ttttatttga attccaaact caccgacagc gttgaatgtt cactgaagca
     5881 acctgtgagg cagagatgtg ctgatttaaa cagtatctgt gggagtctgg agcaggaggg
     5941 aggcagagga aagcccaatc ctttaaccaa acctgtcaca ctcgagtggt ctcatacaga
     6001 acttataaga ttcccaaatc caaagacatt tcacgtttat ggtgatttcc cagaacacat
     6061 agcgacatgc aaatattggg atccgctagc ttaagtaacg ccattttgca aggcatggaa
     6121 aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat
     6181 atgggccaaa caggatattt gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     6241 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
     6301 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     6361 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     6421 ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtgtat ccaataaacc
     6481 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     6541 attgactacc cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt tggagaacaa
     6601 cattctgagg gtaggagtcg aatattaagt aatcctgact caattagcca ctgttttgaa
     6661 tccacatact ccaatactcc tgaaatagtt cattatggac agcgcagaaa gagctgggga
     6721 gaattgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6781 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6841 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6901 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7861 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7921 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7981 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8041 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8101 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8161 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8221 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8281 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8341 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8401 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8461 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8521 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8581 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8641 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8701 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8821 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8881 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     8941 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     9001 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     9061 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     9121 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     9181 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     9241 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     9301 cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     9361 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     9421 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     9481 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta
     9541 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     9601 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     9661 gaa
//
