LOCUS       sequence-41589-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic GFP tagging in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-GFP-ura4MX6
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..737
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(54..82)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     677..697
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(816..840)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcgacggat ccccgggtta attaacagta aaggagaaga acttttcact ggagttgtcc
       61 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      121 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      181 tacctgttcc atggccaaca cttgtcacta ctttcactta tggtgttcaa tgcttttcaa
      241 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
      301 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      361 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      421 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      481 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      541 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      601 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      661 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      721 tggatgaact atacaaatag ggcgcgccac ttctaaataa gcgaatttct tatgatttat
      781 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      841 ttaggtttta aaacgaaaat tctt
//
