LOCUS       sequence-41590-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic GFP tagging in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-GFP-ura4MX6
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(212..399)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     320..344
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(463..483)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gaatagaaag agtgaatttt tttagacttg taatgataac tatgtacaaa gccaatgaaa
       61 gatgtatgta gatgaatgta aaataccatg tagacaaaca agataaaact tggttataaa
      121 cattggtgtt ggaacagaat aaattagatg tcaaaaagtt tcgtcaatat cacaagagat
      181 ctatattacc ctgttatccc tagcggatct gccggtagag gtgtggtcaa taagagcgac
      241 ctcatactat acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa
      301 ttttcgtttt aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac
      361 ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag tggcgcgccc
      421 tatttgtata gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg
      481 accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa
      541 tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct
      601 gctagttgaa cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca
      661 ttcttttgtt tgtctgccat g
//
