LOCUS       sequence-415900-       11357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and
            EmGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Makri Pistikou AM, Cremers GAO, Nathalia BL, Meuleman TJ, Bogels 
            BWA, Eijkens BV, de Dreu A, Bezembinder MTH, Stassen OMJA, Bouten 
            CCV, Merkx M, Jerala R, de Greef TFA
  TITLE     Engineering a scalable and orthogonal platform for synthetic 
            communication in mammalian cells.
  JOURNAL   Nat Commun. 2023 Nov 2;14(1):7001. doi: 10.1038/s41467-023-42810-5.
  PUBMED    37919273
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-11-2"; volume: "14(1):7001. doi"; pages: " 
            10.1038/s41467-023-42810-5"
COMMENT     Sequence Label: pHR-SUMO-A'-l4-G-IRES-EmGFP
FEATURES             Location/Qualifiers
     source          1..11357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(15..33)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(16..33)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(323..342)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(556..575)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     675..697
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(735..753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        821..925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(926..994,995..1786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1144..1163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1957..2545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2446..2465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2699..2716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2786..2806)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2791..3120
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2971..3106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4254..4319
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4449..4469
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4894..5028
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4931..4950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4985..5004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5196..5829
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5876..6001
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6498..6731
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6916..6960
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7245..7362
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7483..7862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7863..8066
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8017..8037
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    8068..8086
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8089..8107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      8171..8180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8909..8932
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(8945..8950,8951..8974,8975..9010,9011..9034)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     misc_feature    join(9049..9623,9624..9626,9627..9635)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9216..9233)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9443..9462
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9636..9638,9639..9641,9642..10355)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9681..9702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9942..9961)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10289..10310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10416..11004
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10469..10489)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10887..10898)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11114..11347
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
       61 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
      121 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
      181 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
      241 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
      301 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      361 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      421 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
      481 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
      541 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
      601 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      661 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      721 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      781 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1081 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1141 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1201 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1261 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1321 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1381 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1441 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1501 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1561 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1621 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1681 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1741 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1801 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1861 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2101 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2401 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2461 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2521 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2581 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2641 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2701 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2761 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2821 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2941 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3001 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3061 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3121 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
     3181 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
     3241 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
     3301 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
     3361 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
     3421 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
     3481 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
     3541 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggattccggt
     3601 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
     3661 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
     3721 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
     3781 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
     3841 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
     3901 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
     3961 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
     4021 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
     4081 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
     4141 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
     4201 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     4261 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     4321 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     4381 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     4441 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     4501 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     4561 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     4621 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     4681 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     4741 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
     4801 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4861 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     5041 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     5101 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     5161 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     5221 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     5281 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     5341 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     5401 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     5461 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     5521 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     5581 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     5641 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5821 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     5881 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     5941 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     6001 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     6061 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     6121 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     6181 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     6241 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     6301 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     6361 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     6421 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     6481 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     6541 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     6601 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     6661 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     6721 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     6781 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     6841 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     6901 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     6961 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     7021 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     7081 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     7141 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     7201 aacggatctc gacggtatcg ccaaatggca gtattcatcc acaattttaa aagaaaaggg
     7261 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     7321 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     7381 agcagagatc cagtttggat cgataagctt gatatcgaat tgctgcagcc ccgatagtcg
     7441 actacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
     7501 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     7561 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     7621 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     7681 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     7741 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     7801 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     7861 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
     7921 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     7981 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
     8041 ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg
     8101 atagagatcg tcgtcgtgct cgtttagtga accgtctgat ctcaacaagc tgtctagaga
     8161 attcaagctt gccaccatgg gtatccttcc cagccctggg atgcctgcgc tgctctccct
     8221 cgtgagcctt ctctccgtgc tgctgatggg ttgcgtagct gaaaccggta gcagcggcac
     8281 tagtagcggc ggtggtagcg atagcgaagt caatcaagaa gcgaaaccgg aagtcgaacc
     8341 ggaagtcaaa ccggaaacgc acatcaacct gaaagttagt gatggcagct ctgaaatttt
     8401 ctttaaaatt aagaaaacca cgccgctgcg tcgcctgatg gaagcgtttg ccaaacgcca
     8461 gggcaaagaa atggatagcc tgcgcttcct gtatgacggt attcgtatcc aggcggatca
     8521 agccccggaa gatctggaca tggaagataa cgacattatc gaagcgcatc gcgaacagat
     8581 tggcggccat atgagcggtc ctggatcctc tccggaagac aaaattgcgc agttgaaaca
     8641 aaaaattcag gcgctcaagc aagaaaatca acagttggag gaggagaatg cagccctgga
     8701 gtatggtgga agtcgaattc ggtgggaatt tcatcacggg ggttcgggtg gttcaggcgg
     8761 cggtacctcc ggccctggca gcagccccga ggacgagatc caagccctgg aagagaagaa
     8821 cgcccagctg aaacaggaga tcgccgctct ggaagaaaag aaccaggccc tgaagtacgg
     8881 cggatctaag ctttggagcc acacgcgtct tgaggtgctg tttcagggac caggaggtag
     8941 tggatctgct tggagccatc cacagttcga aaaaggtgga ggttctggcg gtggatcagg
     9001 tggaagtgca tggtctcacc ctcagtttga gaaataatga ctcgagcgcc cctctccctc
     9061 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     9121 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     9181 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     9241 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     9301 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     9361 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     9421 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     9481 tgcccagaag gtaacccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     9541 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     9601 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
     9661 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     9721 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     9781 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
     9841 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     9901 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     9961 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
    10021 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
    10081 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
    10141 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
    10201 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
    10261 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
    10321 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct tgagtcgagc
    10381 tgcaggcatg caagcttgat atcgtacgta tcgataatca acctctggat tacaaaattt
    10441 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
    10501 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
    10561 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
    10621 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
    10681 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
    10741 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
    10801 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
    10861 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
    10921 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
    10981 tctccctttg ggccgcctcc ccgcatcgat accgtcgtcc acgagggaat taattcgagc
    11041 tccgtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    11101 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
    11161 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    11221 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    11281 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    11341 tctagcagca actagaa
//
