LOCUS       sequence-41591-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU
  TITLE     Double conditional human embryonic kidney cell line based on FLP and
            PhiC31 mediated transgene integration.
  JOURNAL   BMC Res Notes. 2011 Oct 18;4:420.
  PUBMED    22008483
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2011-10-18"; volume: "4"; pages: "420"
COMMENT     Sequence Label: pINT-PuroDDHNF4a2
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..51
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     33..50
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(89..91,92..412)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
ORIGIN
        1 ccattctgcc tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct
       61 acttgttctt tttgcaggat ccgccaccat gggagtgcag gtggaaacca tctccccagg
      121 agacgggcgc accttcccca agcgcggcca gacctgtgtg gtgcactaca ccgggatgct
      181 tgaagatgga aagaaagtcg attcctcccg ggacagaaac aagcccttta agtttatgct
      241 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
      301 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat
      361 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaaccgg aagaattccg
      421 actctccaaa accctcgtcg acatggacat ggccgactac agtgctgcac tggacccagc
      481 ctacaccacc ctggaatttg agaatgtgca ggtgttgacg atgggcaatg acacgtcccc
      541 atcagaaggc accaacctca acgcgcccaa cagcctgggt gtcagcgccc tgtgtgccat
      601 ctgcggggac cgggccacgg gcaaacacta cggtgcctcg agctgtgacg gctgcaaggg
      661 cttcttccgg aggagcgtgc ggaagaacca catgtactcc tgcagattta gccggcagtg
      721 cgtggtggac aaagacaaga ggaaccagtg ccgctactgc a
//
