LOCUS       sequence-41592-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic GFP tagging in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-GFP-bleMX6
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..744
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(61..89)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     684..704
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacagtaaag gagaagaact tttcactgga
       61 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      121 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      181 ggaaaactac ctgttccatg gccaacactt gtcactactt tcacttatgg tgttcaatgc
      241 ttttcaagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa
      301 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      361 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      421 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      481 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      541 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      601 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      661 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      721 catggcatgg atgaactata caaatagggc gcgccacttc taaataagcg aatttcttat
      781 gatttatgat ttttattatt aaataagtta t
//
