LOCUS       sequence-415920-       13065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible plasmid for TurboID-based proximity labelling 
            experiment in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Tan H, Zhou Y, Dinius E, Lozano-Duran R
  TITLE     The Ti-TAN plasmid toolbox for TurboID-based proximity labeling 
            assays in Nicotiana benthamiana.
  JOURNAL   J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610.
  PUBMED    38294100
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610."
COMMENT     Sequence Label: pGW-TurboID-GOI
FEATURES             Location/Qualifiers
     source          1..13065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1181..1207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1211..1237
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1241..1267
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1274..2230
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    2234..2358
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2394..2399,2400..2417,2418..2424)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2478..3137
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2544..2563)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3479..3784
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3663..3682
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3825..3949)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3987..4239
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4272..4288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4272..4288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4285..4307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4296..4312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4320..4326,4327..4344,4345..4350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4365..4386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4422..4674)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4825..5850)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5902..6081)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6587..6611
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7132..7923
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8169..8757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8658..8677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8911..8928
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8943..9083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9009..9028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9169..9191
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9427..9621
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9687..10760)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11189..11818)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg tacccgtatg atgttccgga
     1201 ttacgctggc tatccctacg acgtgcccga ctatgccggg tacccctatg acgtcccaga
     1261 ctacgcagct agcaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg
     1321 cgagttccat agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa
     1381 caagcacatt cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg
     1441 ctactctctg cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga
     1501 cggcgggagc gtggcagtcc tgcctgtggt agactccacc aatcagtacc tgctggatcg
     1561 aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg
     1621 aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg
     1681 gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc
     1741 agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta
     1801 tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc
     1861 tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt
     1921 ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct
     1981 ggccgctacg ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct
     2041 ggctccatat ctgccacggt gggagaagct ggataacttc atcaatagac ccgtgaagct
     2101 gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct
     2161 gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc
     2221 tgccgaaaag atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     2281 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     2341 acatatccag tcactatggc ggccctcgag gggccgcatt aggcacccca ggctttacac
     2401 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     2461 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     2521 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     2581 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     2641 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     2701 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     2761 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     2821 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     2881 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     2941 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     3001 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     3061 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     3121 ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt
     3181 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     3241 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     3301 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     3361 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     3421 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     3481 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     3541 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     3601 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     3661 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     3721 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     3781 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     3841 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     3901 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg atgtacaagt
     3961 aaagcggccc gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     4021 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     4081 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     4141 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     4201 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa
     4261 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4321 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4381 acattaggct gaattaggcg cgcctatttc tattaattcc cgatctagta acatagatga
     4441 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
     4501 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
     4561 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
     4621 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
     4681 attcggggga tcgatcccgc gaactgtgga cgagaactgt gccaccaagc gtaaggccgt
     4741 tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt ctcgaaatca
     4801 gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc tggggcgtcg
     4861 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     4921 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     4981 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     5041 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
     5101 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     5161 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
     5221 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
     5281 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     5341 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     5401 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     5461 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
     5521 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
     5581 gcaataggtc aggctctcgc tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc
     5641 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     5701 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     5761 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
     5821 agacgtcgcg gtgagttcag gctttttcat atcttattgc cccccgggat cagatctgga
     5881 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
     5941 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
     6001 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg
     6061 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     6121 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca
     6181 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     6241 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     6301 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     6361 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     6421 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
     6481 agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca
     6541 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     6601 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     6661 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     6721 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     6781 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     6841 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     6901 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     6961 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     7021 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     7081 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     7141 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     7201 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     7261 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     7321 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     7381 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     7441 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     7501 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     7561 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     7621 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     7681 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     7741 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     7801 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     7861 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     7921 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     7981 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     8041 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     8101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8281 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8821 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8881 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8941 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     9001 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     9061 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     9121 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9181 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     9241 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     9301 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     9361 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     9421 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     9481 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     9541 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     9601 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
     9661 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     9721 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     9781 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     9841 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     9901 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
     9961 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    10021 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    10081 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    10141 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    10201 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    10261 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    10321 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    10381 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    10441 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    10501 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    10561 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    10621 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    10681 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    10741 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    10801 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    10861 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    10921 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    10981 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    11041 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    11101 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    11161 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    11221 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    11281 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    11341 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    11401 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    11461 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    11521 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    11581 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    11641 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    11701 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    11761 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    11821 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    11881 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    11941 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    12001 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    12061 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    12121 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    12181 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    12241 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    12301 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    12361 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    12421 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    12481 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    12541 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    12601 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    12661 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    12721 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    12781 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    12841 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    12901 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    12961 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    13021 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aacct
//
