LOCUS       sequence-41593-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic GFP tagging in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-GFP-bleMX6
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(76..273)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(279..653)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVSDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVDEEQD"
ORIGIN
        1 cactagtgga tctgatatca tcgatgaatt cgagctcgtt taaactggat ggcggcgtta
       61 gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac
      121 tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat
      181 cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa
      241 gttcttgaaa acaagaatct ttttattgtc agtactgatc agtcctgctc ctcgtccacg
      301 aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg
      361 ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac
      421 cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccgac
      481 accacctggt cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg
      541 aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct
      601 ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggttgtt
      661 tatgttcgga tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa
      721 agaagaacct cagtggcaaa tcctaacctt t
//
