LOCUS       sequence-415931-       12407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat Cav2.1 with an exofacial double HA tag in domain 
            II and mutations in the P loop of Domains I, II, III and IV which 
            abolish trafficking to the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12407)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 12407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A,...
FEATURES             Location/Qualifiers
     source          1..12407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          658..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3486..3512
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3516..3542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8319..8348
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(9425..9444)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9490..9545
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9544..9563)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9906..9922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9906..9922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9919..9941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9930..9946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9954..9960,9961..9978,9979..9984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9999..10020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10079..10274
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10125..10260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10280..10414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10317..10336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10371..10390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10482..10499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10653..11241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10733..10752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11412..12203,12204..12272))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12035..12054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12273..12377)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggsgsgssr kgsggggsgg ggsggggsgr ggggsggggs
      541 ggggsgrggs ggrgrggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggkgg cggcrggkgg gggtgccggg cggggcgggg
     1261 ccgcckcggg csggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccattgt tcattgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gcattgcggc
     1741 cgcttagacc ggtctcgagg tcttgcagaa tggcccgctt tggagacgag atgccgggcc
     1801 gctacggcgc aggcggagga ggctcagggc cggccgccgg ggtggtcgtg ggcgccgcgg
     1861 gcggccgagg agccgggggc agccggcagg gcgggcagcc cggagcgcag aggatgtaca
     1921 agcagtcgat ggcgcagaga gcgcggacca tggccctcta caaccccatc cctgtccgcc
     1981 agaactgcct cacggtcaac cgctccctgt tcctcttcag tgaagacaac gtggtgagaa
     2041 aatacgccaa aaagatcacg gaatggcctc ccttcgagta catgatcctg gccaccatca
     2101 ttgctaactg catcgtcctg gccctggagc agcacctccc tgatgatgac aagacaccca
     2161 tgtccgagcg gctggatgac acagaaccct atttcattgg catcttctgt tttgaggctg
     2221 gaattaagat cgtggctctt ggctttgcct tccacaaagg ctcctacctg aggaatggct
     2281 ggaacgtcat ggactttgtc gtggtgctaa caggcatctt ggccactgtc gggacggagt
     2341 ttgatctacg gacactgagg gcggttcgtg tgctgcggcc actcaagctg gtgtctggaa
     2401 tcccaagttt acaagtcgtc ctgaagtcaa tcatgaaggc gatgatccct ctgctgcaga
     2461 tcggcctcct cctgtttttt gcaatcctta tttttgcaat catagggtta gaattttata
     2521 tgggaaaatt tcataccacc tgctttgaag aggggacaga cgacatccag ggtgagtcgc
     2581 cagctccgtg tgggacagag gagcctgccc gcacctgccc caacgggacc aaatgtcagc
     2641 cgtactggga agggcccaac aacggcatca ctcagttcga caacatcctg tttgctgtgc
     2701 tcactgtttt ccagtgcatc accatggcag gctggactga tctcctctac aatagcaacg
     2761 atgcctcagg gaacacttgg aactggttgt acttcatccc cctcatcatc atcggctcct
     2821 tttttatgct gaaccttgtg ctgggtgtgc tgtctgggga gtttgccaaa gaaagggaac
     2881 gtgtagagaa ccgaagggct tttctgaagc tcagaagaca acagcagatt gaacgtgagc
     2941 tcaatggata catggagtgg atctcgaaag cagaagaggt gatcctcgcg gaggacgaaa
     3001 cagacgtgga gcagaggcac ccttttgatg gagctcttcg gagagctact ctgaagaaaa
     3061 gcaagacgga cctgctcaac cctgaggagg cggaggacca gcttgctgac atcgcgtctg
     3121 tggggtctcc cttcgccaga gccagcatca aaagtgccaa gctggagaat tcgacttttt
     3181 tccacaaaaa ggagagaaga atgcgtttct acatccgccg catggtcaaa actcaggcct
     3241 tctactggac cgtgctcagt ctggtagccc tcaacacgct gtgcgtcgcc attgtccact
     3301 acaaccagcc cgagtggctc tccgacttcc tctactatgc agaattcatt ttcttaggac
     3361 tctttatgtc cgaaatgttt ataaaaatgt atgggctcgg gacacggcct tacttccact
     3421 cttccttcaa ctgctttgac tgtggggtca tcatcgggag catctttgaa gtcatctggg
     3481 ccgtctaccc ctacgacgtc cccgactacg ccggctaccc gtacgacgtc ccggactacg
     3541 ccggcgtcga catcaaaccg ggtacatcct ttggaatcag cgtgttacga gctctcaggt
     3601 tactgcgtat tttcaaagtc acaaagtact gggcatctct cagaaacctg gttgtctccc
     3661 tcctcaactc catgaaatcc atcataagtc tgctgttcct cctcttcctc ttcattgtcg
     3721 tctttgccct cttggggatg cagctgtttg gtggccagtt taattttgac gaggggactc
     3781 ctcccaccaa cttcgacact tttccagcag caataatgac tgtgtttcag atcctgactg
     3841 gcgcggattg gaatgaggtc atgtatgatg ggatcaagtc tcaggggggc gtgcagggcg
     3901 gcatggtgtt ctccatctac ttcatcgtcc tcaccctctt cgggaactac accctgctga
     3961 acgtgttctt ggctatcgcg gtggacaacc tggccaacgc ccaggaactc accaaggatg
     4021 aacaagaaga ggaagaggca gccaatcaga aactggctct acagaaagcc aaggaggtgg
     4081 cagaagtgag tcccctgtct gcagccaaca tgtccatagc tgtgaaggaa cagcagaaga
     4141 accagaagcc tgccaagtcg gtgtgggagc agcgcaccag cgagatgcgc aagcagaacc
     4201 tgctggctag ccgcgaggcg ctgtacgggg acgcggctga gcgctggccc accacttacg
     4261 cgcgcccgct gcggccggac gtgaagacgc acttggaccg gccgctcgtg gtggacccgc
     4321 aggagaaccg taacaacaac accaacaaga gccgtgcgcc agaagcgctg cgccaaaccg
     4381 cgcggccccg cgagagcgcg cgcgaccccg acgcgcggcg cgcctggccc agcagccctg
     4441 agcgcgcccc tggacgagag ggcccgtatg gccgcgagag cgagccgcaa cagcgcgagc
     4501 acgcgccacc ccgcgagcac gtaccctggg acgcggatcc tgagcgcgcc aaggccgggg
     4561 acgcgccccg ccgccacacg caccggcctg tggccgaggg cgagcctcgt cgccaccgcg
     4621 cgcgccgccg gcccggggac gaaccggacg acagaccgga gcgcaggccg cgtccccgcg
     4681 acgccactag gccggcccgc gctgcagacg gcgaaggcga tgatggggag cgcaagcggc
     4741 gacaccgaca cgggccgccg gcccacgatg acagggagcg cagacaccgg cgcagaaaag
     4801 agagccaggg ctctggggtc cccatgtctg gtcccaacct gtccaccacc aggccaatcc
     4861 agcaggatct gggccgccag gacctgccac tggctgagga cctggacaac atgaagaaca
     4921 acaagttggc caccggggag cctgccagtc cccacgacag cctgggccac agtggccttc
     4981 cccctagccc tgccaagatc gggaacagca ccaaccctgg tcccgccttg gccaccaatc
     5041 cccagaatgc tgccagccgc aggacgccca acaacccggg caacccgtcc aaccccggcc
     5101 cccccaagac tcccgagaac agccttatcg tcaccaaccc cagcagcacc cagcccaact
     5161 cagcaaagac tgccaggaaa cccgagcaca tggcggtgga gatccccccg gcctgcccgc
     5221 ccctcaacca cactgtggtc caagtaaaca aaaacgccaa cccagaccca ctgccaaaga
     5281 aagaggaaga gaagaaggag gaagaggagg cagaccccgg ggaggatggc ccaaagccca
     5341 tgccgcccta cagctccatg ttcatcctct ccaccaccaa cccccttcgc cggctgtgcc
     5401 attacatcct gaacctgcgc tacttcgaga tgtgcatcct catggtcatt gccatgagta
     5461 gcatcgcgct ggccgccgag gacccggtgc agcccaacgc accccgcaac aacgtgctgc
     5521 gatattttga ctatgttttc acaggagtgt ttacctttga gatggtgatc aagatgatcg
     5581 acctgggcct cgtcctgcat cagggggcct atttccgtga cctgtggaac attctggact
     5641 tcatagtggt cagtggggcc ctggtggcct ttgccttcac tggcaatagc aaaggaaagg
     5701 acatcaacac catcaagtcc ctccgagtcc tccgggtgct acgacctcta aagaccatca
     5761 agcggctgcc taagttgaag gccgtatttg actgcgtggt gaactcgctc aagaacgtct
     5821 tcaacatcct cattgtctac atgctcttca tgttcatctt cgccgtggtg gccgtgcagc
     5881 tcttcaaggg caaattcttc cactgcacgg acgagtccaa ggagtttgag agagactgtc
     5941 gaggcaaata cctcctttac gagaagaacg aggtaaaggc gcgggaccgc gagtggaaga
     6001 aatacgactt ccactacgac aacgtgctct gggccctgct cacgctcttt acggtgtcca
     6061 cgggagcggg ctggccacag gtcctcaagc actcagtgga tgccactttt gagaaccagg
     6121 gccccagccc cgggtaccgc atggaaatgt ccatcttcta cgtggtctac tttgtggtgt
     6181 ttcccttctt ctttgtcaat atctttgtgg ccttgatcat catcaccttc caggagcagg
     6241 gagacaagat gatggaagaa tacagcctag agaaaaatga gagggcctgc atcgactttg
     6301 ccatcagtgc caagccgctg accaggcaca tgccccagaa caagcagagc ttccagtatc
     6361 gaatgtggca gttcgtggtg tccccaccct ttgagtacac catcatggcc atgatcgctc
     6421 tcaacaccat cgtgctaatg atgaagttct atggagcctc tgtggcctat gaaaacgccc
     6481 ttcgagtgtt caacattgtc ttcacctccc tcttctctct cgaatgtgtg ctcaaagtca
     6541 tggcttttgg gattctgaat tatttccgcg atgcctggaa catcttcgac tttgtgactg
     6601 ttctgggcag catcacagac atcctcgtca ccgagtttgg gaataacttc atcaacctga
     6661 gctttctccg cctcttccgt gctgcccgac tcatcaaact cctccgccag ggttacacca
     6721 tccgcattct cctctggact ttcgtgcagt ctttcaaggc cctaccttat gtctgtctgc
     6781 tgatcgccat gctcttcttc atctatgcca tcatcgggat gcaggtgttt ggcaacatcg
     6841 gcattgatgg ggaagatgag gacagcgatg aggatgagtt ccaaatcacg aggcacaata
     6901 acttccggac cttcttccaa gctctcatgc ttctctttcg gagcgccaca ggggcagcgt
     6961 ggcacaacat catgctgtcc tgcctcagcg ggaagccatg cgacaagaac tccgggatcc
     7021 aaaaaccaga gtgtggcaac gagttcgcct atttttactt tgtctcgttc atcttccttt
     7081 gctcatttct gatgctgaat ctctttgttg ctgtcatcat ggacaacttc gagtacctca
     7141 cccgagattc ctccatcctg ggcccccacc acctggatga gtacgtgcgt gtctgggcag
     7201 agtatgaccc tgctgcctgc ggccgcattc actataagga catgtacagt ttattgcgag
     7261 taatatcgcc ccctctcggc ttaggcaaga aatgtcctca tagggttgct tgcaagaggc
     7321 tcttgcggat ggacctaccc gtagcggatg acaacaccgt tcacttcaac tccaccttga
     7381 tggctctgat ccgaaccgcc ctggatatca aaatcgcaaa gggtggagct gacaagcagc
     7441 aaatggacgc agagctccgc aaggaaatga tggccatttg gcccaacctg tctcagaaga
     7501 ccttggatct gctggtcaca cctcacaagt ccacggacct gacagtgggt aagatctacg
     7561 cagccatgat gatcatggag tactaccggc agagcaaggc caagaagctg caggccatgc
     7621 gagaggagca gaaccggaca ccactcatgt tccagcgcat ggagcctcca tcgccaacac
     7681 aggagggagg acccagccaa aacgcccttc cctccactca gctggacccc ggaggaggcc
     7741 tgatggctca agaaagcagc atgaaggaga gcccgtcctg ggtgacccag cgggcacagg
     7801 agatgttcca gaagactggt acctggagcc cagagcgagg gccacccatc gacatgccta
     7861 acagccagcc caactcccag tctgtggaga tgagagaaat gggaactgat ggctactcag
     7921 acagcgaaca ctacctcccc atggaaggac agaccagggc cgcctccatg ccccgcctcc
     7981 cagcagagaa ccagaggaga aggggccggc cacgtggaaa taacctcagt accatctctg
     8041 ataccagccc catgaagcgc tcagcctccg tgctgggacc caaagcccgg cgactggatg
     8101 actactcact agagcgggta ccacctgagg agaaccaaag gtaccaccaa cgccgccggg
     8161 accgtggcca ccgcacctct gagcgctctc tgggccgata cactgatgtg gacacaggcc
     8221 tggggacaga tctgagcatg accacccaat cgggtgacct gccctccaaa gatcgggacc
     8281 aggaccgggg ccggcccaag gaccggaagc atcggccaca ccaccaccac caccatcatc
     8341 accatcatcc cccggccccg gaccgggagc gctacgcaca ggagcggccg gacaccggcc
     8401 gggcgcgggc ccgggagcag cgctggtccc gctcgcccag cgagggtcgg gagcacgcga
     8461 cacacagaca gtagttccgt cagtggaagc ccagccccct ctacgtccgg caccagcacg
     8521 ccgcggcgcg gccgccgcca gctcccccag accccctgca ccccgcggcc gcttgtgtcc
     8581 tactcgcccg ctccgcgcag gcctgcggcg cgcaggatgg cgggtccccc agcgccccct
     8641 ggcggctcgc cgcggggttg ccgccgtgcg ccgcgctggc ctgcgcatgc gcctgagggc
     8701 ccgcggccgc gcggtgccga ctacaccgag cccgacagcc cgcgcgagcc gccagggggc
     8761 gcgcatgagc ccgcgccgcg gtcgcccagg actccgcgcg ccgcgggctg cgcgtcaccg
     8821 cgccacggcc gcaggctgcc caatggttac tacgcggggc acggcgcgcc acggccgcgc
     8881 acagcccgca ggggcgcgca cgacgcgtac agcgagagcg aggacgactg gtgctaagcc
     8941 gccccgcccc ccgcagcgcc cgtacggtgc gggcgcaccg caaatgataa acaataacgt
     9001 ttttaaaaaa gaaaaccggg gagaaaaaca aaaatagctt ctattgatga gttttatcat
     9061 ctcgattgaa tctttcgttt tcccggtgaa cgaaaccgag gtggctgcga gccggaggga
     9121 cggctgcacg ccccacacac ttctcagacc cacgggagtt cttgcgggcc acgccaaact
     9181 tattactgcc tgcagagttc aactacaaga aaataacact aacagttttt aaaaggactc
     9241 cgtaaaaaga aatttgaaag agaatgtaga gaggctgaga aagcccagga attcctgcag
     9301 cccgggggat ccactagtct cgaggaattc ggtaccgagc tcaattcact cctcaggtgc
     9361 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     9421 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     9481 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     9541 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     9601 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     9661 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     9721 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     9781 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     9841 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
     9901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9961 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10021 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
    10081 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    10141 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    10201 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    10261 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10381 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
    10441 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10501 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10561 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10621 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10681 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10741 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10801 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10861 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10921 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10981 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    11041 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    11101 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    11161 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    11221 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    11281 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    11341 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    11401 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    11461 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11521 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    11581 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11641 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11701 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11761 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11821 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11881 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11941 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    12001 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    12061 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    12121 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    12181 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    12241 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    12301 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    12361 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtc
//
