LOCUS       sequence-415937-       13779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible plasmid for TurboID-based proximity labelling 
            experiment in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Tan H, Zhou Y, Dinius E, Lozano-Duran R
  TITLE     The Ti-TAN plasmid toolbox for TurboID-based proximity labeling 
            assays in Nicotiana benthamiana.
  JOURNAL   J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610.
  PUBMED    38294100
REFERENCE   2  (bases 1 to 13779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610."
COMMENT     Sequence Label: pGW-GFP-TurboID-GOI
FEATURES             Location/Qualifiers
     source          1..13779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1178..1180,1181..1183,1184..1891)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(1484..1503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1831..1852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1898..1924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1928..1954
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1958..1984
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1991..2947
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    2948..3072
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3108..3113,3114..3131,3132..3138)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3192..3851
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3258..3277)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4193..4498
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4377..4396
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4539..4663)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4701..4953
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4986..5002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4986..5002)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4999..5021)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5010..5026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5034..5040,5041..5058,5059..5064))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5079..5100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5136..5388)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5539..6564)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6616..6795)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7301..7325
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7846..8637
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8883..9471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9372..9391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9625..9642
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9657..9797
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9723..9742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9883..9905
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10141..10335
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10401..11474)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11903..12532)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact cacggcatgg acgagctgta catcatgtac ccgtatgatg ttccggatta
     1921 cgctggctat ccctacgacg tgcccgacta tgccgggtac ccctatgacg tcccagacta
     1981 cgcagctagc aaagacaata ctgtgcctct gaagctgatc gctctcctgg ctaatggcga
     2041 gttccatagt ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa
     2101 gcacattcag actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta
     2161 ctctctgccc gagcctatcc cgctgctgaa cgctaaacag attctgggac agctggacgg
     2221 cgggagcgtg gcagtcctgc ctgtggtaga ctccaccaat cagtacctgc tggatcgaat
     2281 cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag
     2341 cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg
     2401 cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga
     2461 agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct
     2521 gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc
     2581 acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt
     2641 caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc
     2701 cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc
     2761 tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat
     2821 cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct
     2881 gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc
     2941 cgaaaagaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     3001 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     3061 ccagtcacta tggcggccct cgaggggccg cattaggcac cccaggcttt acactttatg
     3121 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
     3181 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     3241 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     3301 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     3361 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     3421 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     3481 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     3541 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     3601 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     3661 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     3721 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     3781 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     3841 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
     3901 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     3961 gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     4021 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     4081 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     4141 cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt
     4201 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     4261 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     4321 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     4381 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     4441 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     4501 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     4561 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     4621 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatgtac aagtaaagcg
     4681 gcccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     4741 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     4801 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     4861 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     4921 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     4981 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5041 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5101 ggctgaatta ggcgcgccta tttctattaa ttcccgatct agtaacatag atgacaccgc
     5161 gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta
     5221 taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt
     5281 aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc
     5341 aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggaaattcgg
     5401 gggatcgatc ccgcgaactg tggacgagaa ctgtgccacc aagcgtaagg ccgttctctc
     5461 gcatttgcct tgctaggctc gcgcgagttg ctggctgagg cgttctcgaa atcagctctt
     5521 gttcggtcgg catctactct attcctttgc cctcggacga gtgctggggc gtcggtttcc
     5581 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     5641 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     5701 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     5761 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
     5821 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     5881 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     5941 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     6001 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     6061 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     6121 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     6181 gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg
     6241 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     6301 ggtcaggctc tcgctgaatg ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     6361 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     6421 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     6481 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     6541 cgcggtgagt tcaggctttt tcatatctta ttgccccccg ggatcagatc tggattgaga
     6601 gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt
     6661 ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat
     6721 ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct
     6781 tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg
     6841 ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc gccatcagat
     6901 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     6961 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     7021 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     7081 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     7141 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagctga
     7201 tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa
     7261 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     7321 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     7381 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     7441 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     7501 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     7561 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     7621 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     7681 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     7741 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     7801 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     7861 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     7921 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     7981 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     8041 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     8101 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     8161 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     8221 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     8281 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     8341 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     8401 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     8461 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     8521 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     8581 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     8641 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     8701 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     8761 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     8821 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8881 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8941 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9001 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     9061 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9121 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9181 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9241 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9301 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9361 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9421 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9481 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9541 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9601 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     9661 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     9721 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     9781 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9841 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9901 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     9961 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
    10021 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
    10081 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
    10141 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
    10201 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
    10261 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
    10321 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
    10381 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
    10441 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
    10501 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
    10561 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
    10621 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
    10681 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
    10741 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
    10801 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
    10861 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    10921 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    10981 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    11041 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    11101 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    11161 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    11221 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    11281 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    11341 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    11401 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    11461 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    11521 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    11581 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    11641 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    11701 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    11761 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    11821 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    11881 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    11941 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    12001 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    12061 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    12121 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    12181 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    12241 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    12301 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    12361 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    12421 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    12481 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    12541 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    12601 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    12661 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    12721 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    12781 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    12841 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    12901 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    12961 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    13021 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    13081 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    13141 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    13201 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    13261 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    13321 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    13381 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    13441 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    13501 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    13561 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    13621 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    13681 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    13741 ggttggcatg cacatacaaa tggacgaacg gataaacct
//
