LOCUS       sequence-415939-       13069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible plasmid for TurboID-based proximity labelling 
            experiment in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Tan H, Zhou Y, Dinius E, Lozano-Duran R
  TITLE     The Ti-TAN plasmid toolbox for TurboID-based proximity labeling 
            assays in Nicotiana benthamiana.
  JOURNAL   J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610.
  PUBMED    38294100
REFERENCE   2  (bases 1 to 13069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610."
COMMENT     Sequence Label: pGW-GOI-TurboID
FEATURES             Location/Qualifiers
     source          1..13069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2104
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2424..2729
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2608..2627
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2770..2894)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2914..2940
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2944..2970
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2974..3000
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3007..3963
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     terminator      3991..4243
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4276..4292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4276..4292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4289..4311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4300..4316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4324..4330,4331..4348,4349..4354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4369..4390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4426..4678)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4829..5854)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5906..6085)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6591..6615
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7136..7927
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8173..8761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8662..8681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8915..8932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8947..9087
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9013..9032)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9173..9195
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9431..9625
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9691..10764)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11193..11822)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgagaggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca ggcggggcgt aatctagagg atccggctta
     2101 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2161 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2221 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2281 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2341 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2401 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2461 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2521 tgatccccct ggccagtgca cgtctgctgt cagataaagt cccccgtgaa ctttacccgg
     2581 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2641 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2701 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2761 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     2821 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2881 ttgtacaaag tggttcgatc tagaggatcc atgtacccgt atgatgttcc ggattacgct
     2941 ggctatccct acgacgtgcc cgactatgcc gggtacccct atgacgtccc agactacgca
     3001 gctagcaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa tggcgagttc
     3061 catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat caacaagcac
     3121 attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa gggctactct
     3181 ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct ggacggcggg
     3241 agcgtggcag tcctgcctgt ggtagactcc accaatcagt acctgctgga tcgaatcggc
     3301 gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag aggaagcaga
     3361 gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt ttggcgcctg
     3421 aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg
     3481 ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct gtatctgcag
     3541 gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga tgctgcacag
     3601 atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat
     3661 cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct
     3721 acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca
     3781 tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt
     3841 ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg
     3901 gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa
     3961 aagtagagcg gcccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     4021 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     4081 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     4141 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     4201 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa
     4261 ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4321 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4381 actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct agtaacatag
     4441 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
     4501 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
     4561 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
     4621 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg
     4681 ggaaattcgg gggatcgatc ccgcgaactg tggacgagaa ctgtgccacc aagcgtaagg
     4741 ccgttctctc gcatttgcct tgctaggctc gcgcgagttg ctggctgagg cgttctcgaa
     4801 atcagctctt gttcggtcgg catctactct attcctttgc cctcggacga gtgctggggc
     4861 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     4921 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     4981 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     5041 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
     5101 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     5161 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     5221 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     5281 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     5341 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     5401 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     5461 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
     5521 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     5581 agatgcaata ggtcaggctc tcgctgaatg ccccaatgtc aagcacttcc ggaatcggga
     5641 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     5701 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     5761 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     5821 cgacagacgt cgcggtgagt tcaggctttt tcatatctta ttgccccccg ggatcagatc
     5881 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     5941 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     6001 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     6061 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     6121 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc
     6181 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     6241 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     6301 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     6361 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     6421 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc
     6481 gtgaagctga tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag
     6541 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     6601 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     6661 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     6721 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     6781 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     6841 ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     6901 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     6961 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     7021 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     7081 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
     7141 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     7201 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     7261 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     7321 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     7381 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     7441 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     7501 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     7561 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     7621 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     7681 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     7741 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     7801 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     7861 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     7921 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     7981 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     8041 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat
     8101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8281 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     8341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8941 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     9001 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     9061 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     9121 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     9181 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     9241 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     9301 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     9361 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     9421 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     9481 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     9541 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     9601 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     9661 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     9721 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     9781 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     9841 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     9901 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     9961 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
    10021 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
    10081 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
    10141 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
    10201 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
    10261 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
    10321 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
    10381 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    10441 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    10501 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    10561 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    10621 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    10681 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    10741 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
    10801 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    10861 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    10921 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    10981 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    11041 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    11101 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    11161 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    11221 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    11281 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    11341 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    11401 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    11461 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    11521 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    11581 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    11641 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    11701 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    11761 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    11821 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    11881 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    11941 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    12001 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    12061 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    12121 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    12181 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    12241 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    12301 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    12361 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    12421 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    12481 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    12541 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    12601 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    12661 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    12721 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    12781 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    12841 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    12901 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    12961 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    13021 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
